Browsing by Author "Azul, Ana Mano"
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- Abordagem conservadora na restauração de dentes endodonciados – caso clínicoPublication . Caetano, André; Antunes, Maria João; Azul, Ana Mano
- Analysis of laboratory adhesion studies in eroded enamel and dentin : a scoping reviewPublication . Costa, Madalena Belmar da; Delgado, António H. S.; Melo, Teresa Pinheiro de; Amorim, Tomás; Azul, Ana ManoAim: To summarize and report laboratory studies of adhesion in eroded substrates, which used bond strength as an outcome measure. To determine the strategies available to overcome bonding difficulties, the quality and consistency of the methodology and to find evidence gaps. Materials and Methods: The present review followed PRISMA-ScR guidelines. A search was conducted on PubMed/Medline, Scopus and EMBASE (Ovid) databases to identify published peer-reviewed papers (2010–2020). For final qualitative synthesis, 29 articles were selected which respected the inclusion criteria. Data charting was carried out, independently, by two reviewers and quality assessment of the articles was performed. Results: The primary studies included fall into four major categories: comparison of restorative materials and application modes, enzymatic inhibitors, surface pretreatments or remineralization strategies. Most studies found evaluated dentin (76%), while 17% evaluated enamel, and 7% evaluated both substrates. The majority of the studies reported an effective intervention (83%). Bond strength to eroded dentin is significantly reduced, while in enamel erosion is beneficial. The bond strength to eroded dentin is material-dependent and favored in systems containing 10-MDP. Great disparities among the erosion models used were found, with citric acid in different concentrations being the preferred method, although standardization is lacking. Conclusions: Adhesives containing 10-MDP show beneficial results in eroded dentin, and surface preparation methods should be considered. Studies which evaluated adhesion to eroded enamel/dentin show high heterogeneity in what concerns aims and methodology. Strategies that focus on remineralizing dentin and strategies to protect bond longevity in this substrate require further research.
- Antioxidant pre-treatments are able to reduce waiting time for restorative treatment after dental bleaching: a microtensile bond strength exploratory studyPublication . Lobo, Sofia; Santos, Inês Caetano; Delgado, António H. S.; Proença, Luís; Polido, Mário; Azul, Ana Mano; Mendes, José JoãoThe aim was to evaluate the effect of different antioxidant agents on the improvement of bond strength to enamel subjected to a whitening procedure. Samples were divided into six groups (n = 10): control; whitening immediately followed by restorative treatment (WHT); whitening and restoration after a 7-day period (WHT_7D); whitening and application of 10% sodium ascorbate (WHT_SA); application of 5% grape seed extract (WHT_GS); and application of 5% green tea (WHT_GT). All groups except for control fwere whitened (Opalescence PF Regular 16%) for 7 days (4 h/day). All samples were restored (Optibond™ FL, Kerr and Filtek™ Z250 composite, 3M ESPE). In antioxidant groups these were applied for 15 min and immediately restored. Specimens were sectioned in microspecimens (1.0 ± 0.2 mm2 area) for microtensile bond strength (μTBS) testing (0.5 mm/min) on a universal testing machine. ANOVA test with Brown-Forsythe correction and Games-Howell post-hoc test was used (significance level of 5%). Antioxidant groups presented significant higher mean μTBS values than group WHT (p < 0.001). The non-whitened control group was not significantly different to antioxidant experimental groups. Group WHT_7D exhibited a significantly higher mean μTBS value when compared to group WHT (p = 0.022). The different antioxidants significantly influenced microtensile bond strength of restored teeth after whitening.
- Collagen-depletion strategies in dentin as alternatives to the hybrid layer concept and their effect on bond strength : a systematic reviewPublication . Delgado, António H. S.; Costa, Madalena Belmar da; Polido, Mário Cruz; Azul, Ana Mano; Sauro, SalvatoreStrategies aiming to improve the longevity of resin–dentin adhesive interface developed so far have only been able to retard the problem. Different approaches are thus needed. The objective of this review was to determine whether the use of collagen-depletion strategies after acid-etching procedures may improve the bond strength of resin-based materials to dentin. A systematic review was planned following 2021 PRISMA statement guidelines, with a search strategy performed in five electronic databases: PubMed/Medline, Scopus, EMBASE, SciELO and IADR Abstract Archive (last search: 17/01/2022). Inclusion criteria encompassed studies which evaluated a collagen-depletion strategy in acid-etched human dentin and tensile/shear bond strength tests. Risk of bias assessment was carried out by two reviewers, working independently on an adapted five-domain risk of bias (RoB) checklist for laboratory studies. Results were synthesized qualitatively, as a meta-analysis was not possible due to limited number of studies and their RoB. A total of eight studies were eligible for inclusion in the systematic review after inclusion/exclusion criteria application. Out of these, two evaluated the effect of using NaOCl followed by an antioxidant, and the remaining six evaluated different enzymatic treatments (bromelain, chondroitinase ABC, papain, and trypsin). None of the studies reported a decrease of bond strength when a collagen-depletion strategy was used, in comparison to traditional hybrid layers (control). All enzymatic treatment studies which respected the inclusion criteria improved the bond strength to dentin. Some specific collagen-depletion strategies seem to play a favorable role in improving immediate bond strengths to dentin. Further research with sound methodology is required to consolidate these findings, since limitations in RoB and a low number of studies were found. The assessment of further proteolytic agents and long-term outcomes is also required.
- Comparative assessment of different pre-treatment bonding strategies to improve the adhesion of self-adhesive composites to dentinPublication . Inglês, Magali; Cruz, Joana Vasconcelos e; Azul, Ana Mano; Polido, Mário; Delgado, António H. S.The aim of this study is to compare the adhesive interface formed in dentin, using self-adhesive composites applied with different bonding strategies, by testing the microtensile bond strength (μTBS) and ultramorphology through the use of light microscopy. Permanent, sound human molars were randomly allocated to six experimental groups. The groups included a negative control group, where only etching was performed via EtchOnly; a positive control group where an adhesive was applied, OptiBondFL (OBFL); and an experimental group where a primer was applied using a co-curing strategy together with a composite (Primer_CoCuring). The samples were sectioned into microspecimens for μTBS (n = 8) and into 1-mm thick slabs for light microscopy using Masson’s trichrome staining protocol (n = 3). The statistical analysis included a two-way ANOVA for μTBS data and Tukey’s HSD was used as a post-hoc test (significance level of 5%; SPSS v. 26.0). The results of the μTBS revealed that the self-adhesive composite (F = 6.0, p < 0.018) and the bonding strategy (F = 444.1, p < 0.001) significantly affected the bond strength to dentin. However, their interactions were not significant (F = 1.2, p = 0.29). Etching dentin with no additional treatment revealed the lowest μTBS (VF_EtchOnly = 2.4 ± 0.8 MPa; CC_EtchOnly = 2.0 ± 0.4 MPa), which was significantly different from using a primer (VF_CoCu = 8.8 ± 0.8 MPa; CC_CoCu = 6.3 ± 1.0 MPa) or using the full adhesive (VF_OptiBondFL = 22.4 ± 0.3 MPa; CC_OptibondFL = 21.2 ± 0.4 MPa). Microscopy images revealed that the experimental Primer_CoCuring was the only group with no collagen fibers exposed to the dentin–composite interface. Overall, the use of a primer, within the limitations of this study, increased the bonding of the self-adhesive composite and provided sufficient infiltration of the collagen based on light-microscopic imaging.
- Diagnóstico e tratamento de fracturas verticais: a propósito de um caso clínicoPublication . Alves, Gustavo; Laranjo, João; Melancia, Tanya; Azul, Ana Mano; Mendes, José João
- Diferenças na microdureza de resinas compostas de núcleo e convencionaisPublication . Silva, Susana Matias Ferreira da; Moura, Pedro Melo e; Polido, Mário Cruz; Azul, Ana Mano
- Efeito da variação do protocolo adesivo na reparação de uma nanocerâmica/resinaPublication . Madeira, Joana Maria de Oliveira; Monteiro, Paulo; Mendes, José João; Polido, Mário; Azul, Ana Mano
- Estilo de vidaPublication . Azul, Ana Mano
- Formação & (cons)ciênciaPublication . Azul, Ana Mano