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Advisor(s)
Abstract(s)
Cabo Verde is an archipelago located on the West African coast. It was discovered in 1460 by Portuguese explorers and it was colonized by them and by Africans. Culturally, Cabo Verde is characterized by an admixture of European and African origin elements, being one of the countries with the highest number of emigrants in Portugal.
PowerPlex® Fusion System kit (Promega Corporation) consists of a multiplex that amplifies 24 loci (D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, VWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA, D22S1045, DYS391 and Amelogenin) in a single PCR reaction. These genetic markers include all Short Tandem Repeats (STRs) encompassed in the Combined DNA Index System (CODIS) and in the European Standard Set (ESS).
In order to implement or modify technologies for forensic DNA analysis there are two types of validation required: developmental and internal. The developmental validation studies accuracy, reproducibility and precision of the procedure by the manufacturer, and it should precede the use of this novel methodology for forensic DNA analysis. Internal validation is performed by each laboratory of forensic DNA analysis to demonstrate that established method and procedure perform as expected in the laboratory.
Parameters such as final volume of PCR reaction, DNA concentration range, stutter peaks ratio and heterozygote peak height ratios, were determined. Beyond the method implementation, one of the aims of this work is sample costs-saving by the laboratory maintaining the quality of the obtained results.
Description
Poster apresentado no 26th Congress of the International Society for Forensic Genetics, Kraków, Polan(2015)
Keywords
Powerplex® Fusion System Cabo Verde immigrant population Lisboa