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Usefulness of Powerplex® Fusion System as typing methodology of Codis and ESS genetic markers in a immigrant population from Cabo Verde in Lisboa

2015Other Open access
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datacite.subject.fosCiências Médicas::Outras Ciências Médicas
dc.contributor.authorResende, Ana
dc.contributor.authorAmorim, António
dc.contributor.authorVieira da Silva, Cláudia
dc.contributor.authorRibeiro, Teresa
dc.contributor.authorMaria João Porto
dc.contributor.authorCosta Santos, Jorge
dc.contributor.authorAfonso Costa, Heloísa
dc.date.accessioned2025-10-06T21:23:13Z
dc.date.available2025-10-06T21:23:13Z
dc.date.issued2015
dc.descriptionPoster apresentado no 26th Congress of the International Society for Forensic Genetics, Kraków, Polan(2015)
dc.description.abstractCabo Verde is an archipelago located on the West African coast. It was discovered in 1460 by Portuguese explorers and it was colonized by them and by Africans. Culturally, Cabo Verde is characterized by an admixture of European and African origin elements, being one of the countries with the highest number of emigrants in Portugal. PowerPlex® Fusion System kit (Promega Corporation) consists of a multiplex that amplifies 24 loci (D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, VWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA, D22S1045, DYS391 and Amelogenin) in a single PCR reaction. These genetic markers include all Short Tandem Repeats (STRs) encompassed in the Combined DNA Index System (CODIS) and in the European Standard Set (ESS). In order to implement or modify technologies for forensic DNA analysis there are two types of validation required: developmental and internal. The developmental validation studies accuracy, reproducibility and precision of the procedure by the manufacturer, and it should precede the use of this novel methodology for forensic DNA analysis. Internal validation is performed by each laboratory of forensic DNA analysis to demonstrate that established method and procedure perform as expected in the laboratory. Parameters such as final volume of PCR reaction, DNA concentration range, stutter peaks ratio and heterozygote peak height ratios, were determined. Beyond the method implementation, one of the aims of this work is sample costs-saving by the laboratory maintaining the quality of the obtained results.por
dc.identifier.urihttp://hdl.handle.net/10400.26/58918
dc.language.isoeng
dc.peerreviewedyes
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPowerplex® Fusion System
dc.subjectCabo Verde immigrant population
dc.subjectLisboa
dc.titleUsefulness of Powerplex® Fusion System as typing methodology of Codis and ESS genetic markers in a immigrant population from Cabo Verde in Lisboaeng
dc.typeother
dspace.entity.typePublication
oaire.citation.title26th Congress of the International Society for Forensic Genetics
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAmorim
person.familyNameda Silva
person.familyNameRibeiro
person.familyNameSantos
person.familyNameAfonso Costa
person.givenNameAntónio
person.givenNameCláudia
person.givenNameTeresa
person.givenNameJorge
person.givenNameHeloísa
person.identifier.ciencia-idD218-0D74-678B
person.identifier.orcid0000-0002-7506-4426
person.identifier.orcid0000-0003-0373-7457
person.identifier.orcid0000-0002-2903-0006
person.identifier.orcid0000-0002-8925-3068
person.identifier.orcid0000-0002-7823-7687
person.identifier.ridA-4414-2013
person.identifier.ridJ-5725-2015
person.identifier.scopus-author-id35253426400
person.identifier.scopus-author-id9839086200
person.identifier.scopus-author-id7402389332
person.identifier.scopus-author-id24729171700
relation.isAuthorOfPublication71c1447c-fe17-438b-bba1-256e3abdb87a
relation.isAuthorOfPublication0513acfa-9182-491c-b57f-f14aa1cdd2e2
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relation.isAuthorOfPublicatione75e9d2d-2282-46d8-a2cb-37b7edbb1156
relation.isAuthorOfPublication.latestForDiscovery71c1447c-fe17-438b-bba1-256e3abdb87a

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