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Publicação
Mitochondrial DNA characterization of Brazilian immigrant Population living in Lisboa
Orientador(es)
Resumo(s)
Migration is one of the main factors for genetic variability within populations. Currently, the Portuguese population, and particularly the population from Lisboa, welcomes a
considerable number of immigrants. Brazilian immigrants are the main foreign community in Portugal, with about 184 000 individuals in 2020.
Mithocondrial DNA (mtDNA), due to its unique characteristics such as being exclusively maternal inheritance and suffering no recombination, which results in its slow evolution, is a useful genetic marker to study the evolution of populations.
In this study mtDNA sequencing analyzis of 64 Brazilian immigrants who currently live in Lisbon were carried out in order to assess the impact of this population on the Portuguese gene pool.
The mtDNA control region were amplified using two pairs of primers - L15971 / H016 and L16555 / H639. The amplified products were then sequenced using BigDye®Terminator
v.3.1 Cycle Sequence (AB) and detected in the SeqStudio™ Genetic Analyzer (AB). The results were analysed with the Sequencing Analysis v7. and SeqScape v4. (AB) softwares, where the obtained sequences were compared with the rCRS in order to obtain haplotypes that, with Phylotree, build 17, can be converted in haplogroups.
Descrição
Poster apresentado no III International Meeting of the Portuguese Society of Genetics, Évora, 27-28 june 2022
Palavras-chave
Mitochondrial DNA Brazilian Immigrant Population Lisbon