INMLCF - Instituto Nacional de Medicina Legal e Ciências Forenses, IP
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O Instituto Nacional de Medicina Legal e Ciências Forenses, I. P., é um instituto público tutelado pelo Ministério da Justiça, tem a natureza de Laboratório do Estado e é considerado instituição nacional de referência na área da medicina legal e ciências forenses. Com sede em Coimbra, dispõe de 3 Delegações (Porto, Coimbra e Lisboa) e uma rede de gabinetes médico-legais e forenses com cobertura no território nacional.
O INMLCF tem como missão assegurar as perícias médico-legais e forenses, a coordenação científica da atividade na área da medicina legal e ciências forenses, promover a formação e investigação, superintender e orientar a atividade dos serviços médico-legais.
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- 1,4-Butanediol intoxication: a case reportPublication . Castro, André Lobo; Tarelho, Sónia; Dias, Ana Sofia; Melo, Paula; Teixeira, Helena; Franco, João MiguelGamma-hidroxibutyric acid (GHB) is an endogenous compound with known action at the neural level. Its psychoactive effects led to an illicit use context including recreational purposes, muscle building effects in bodybuilders and drugs-facilitated crimes, specifically, in sexual assaults. Besides the use of the main compound, there are precursors, like Gammabutyrolactone (GBL) and 1,4-butanediol (1,4-BD), usually non controlled substances, allowing a much easier way to obtain the target-compound. The authors present the first reported intoxication case in Portugal with 1,4-Butanediol, including the quantification of GHB and GHB-GLUC in serum, by GC-MS/MS TQD. A male, 25 years old, entered in coma at the hospital emergency room, with the suspicion of an intoxication due to an unknown liquid ingestion. The forensic toxicology lab was asked to collaborate in order to identify the possible substance(s) involved and provide adequate treatment to the patient. The suspicious liquid and a serum sample were sent by the hospital and analysed by GC-MS-single quadrupole and GC-MS/MS TQD, respectively. A methodology including protein precipitation and GC-MS/MS TQD analysis was used to detect and quantify GHB and GHB-GLUC in serum. Toxicological analysis revealed the presence of 1,4-Butanediol in the liquid and GHB [171 mg/L] and GHB-GLUC [13,7 mg/L] in serum.The victim reverted the coma state with no neurological sequelae. This was the first detected case in Portugal with 1,4-Butanediol, suggesting that it is important to be aware that consumers have different options to acess illicit compounds, such as GHB. On the other hand, GHB-GLUC was identified and quantified for the first time in a real case sample, due to intoxication. This case highlights the importance of analyzing all samples for active compounds, percursors and metabolites that can lead to the main intoxication origin.
- 16 Y-Specific STR Analysis in Human Remains IdentificationPublication . Vieira-Silva, Cláudia; Cruz, C.; Ribeiro, Teresa; Lucas, Isabel; Geada, H.; Espinheira, R.Forensic investigation often requires the use of degraded biological material, especially for determining the identity of human remains. Y-STRs, offer new perspectives for identification and kinship analysis especially on forensic deficiency cases 1 in complement of autosomal STRs. The non-recombining portion of the Y-chromosome is of value providing additional data in paternal lineage identification. The aim was the study of 16 Y-STR loci to perform human remains identification - the minimal Y-STR haplotype - DYS19, DYS390, DYS391, DYS392, DYS393, DYS19, DYS389I/II, DYS385 – and – GATA A .1(DYS460), GATA A 7.2 (DYS461), GATA C4, GATA H4, DYS437, DYS438, DYS439 loci, that are part of the Y-chromosome quality control Group of the Spanish and Portuguese Group (GEPY) of the ISFG. Y-STR typing in degraded biological material has major technical challenges since each sample has unique characteristics. The best results were obtained in autopsy bloodstains may be due to the fact that the autolysis mechanisms are still incipient. Otherwise blood kept at 4º C for more than a year didn’t provide the same good results. Liquid blood samples from deceased individuals may contain porfirinic compounds from hemoglobin, powerful PCR inhibitors difficult to eliminate9. In bone and teeth samples the source of inhibitors may be a high amount of DNA from other micro-organisms such as bacteria and fungi which invade bone9 and probably this kind of contaminants can explain some of the results obtained in bones and teeth samples submitted to prolonged humidity, temperature environmental conditions and also adverse soil characteristics. The problem with the studied samples seems not to be DNA quantity but the out-of-control presence of inhibitors that interfere not only with the extraction but also with the amplification process because most of the results were obtained with a dilution of DNA extraction and adding more Taq, sometimes 2 or 3 fold. With these samples, it seems that the success or failure depends on each sample itself and it should be treated individually. The number of distinct factors connected to each sample is difficult to calculate, so it is necessary to make a few attempts in order to have as many results as possible.
- 16º Congresso Nacional de Medicina Legal e Ciências Forenses : 1ª reunião da rede de serviços médico-legais e forenses de portuguesa : Programa científico, resumosPublication . Instituto Nacional de Medicina Legal e Ciências Forenses
- 17º Congresso Nacional de Medicina Legal e Ciências Forenses : 2ª reunião da rede de serviços médico-legais e forenses de portuguesa : Programa científico, resumosPublication . Instituto Nacional de Medicina Legal e Ciências Forenses
- 18º Congresso Nacional de Medicina Legal e Ciências Forenses: 3ª Reunião da Rede de Serviços Médico-Legais e Forenses de Língua Portuguesa: Programa Científico, ResumosPublication . Instituto Nacional de Medicina Legal e Ciências Forenses
- 19º Congresso Nacional de Medicina Legal e Ciências Forenses: 4ª Reunião da Rede de Serviços Médico-Legais e Forenses de Língua Portuguesa: Programa Científico, ResumosPublication . Instituto Nacional de Medicina Legal e Ciências Forenses
- 20º Congresso Nacional de Medicina Legal e Ciências Forenses: 5ª Reunião da Rede de Serviços Médico-Legais e Forenses de Língua Portuguesa: Programa Científico, ResumosPublication . Instituto Nacional de Medicina Legal e Ciências Forenses
- 21º Congresso Nacional de Medicina Legal e Ciências Forenses: : Programa Científico, ResumosPublication . Instituto Nacional de Medicina Legal e Ciências Forenses
- 22º Congresso Nacional de Medicina Legal e Ciências Forenses: : Programa Científico, ResumosPublication . Instituto Nacional de Medicina Legal e Ciências Forenses
- 5 Anos das agressões sexuais: análise da prevalência e fatores do riscoPublication . Smyk, B.; Ramalho, M.; Mendonça, Ricardo; Teixeira, Helena M.; Carreira, Carla