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Estudo sobre as características estruturais e biológicas do esmalte, onde foram induzidas lesões artificiais de cárie dentária
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Abstract(s)
Objetivos: Qualificar a superfície de esmalte, histologicamente, quando aplicados agentes remineralizadores e uma resina infiltrante de esmalte, comparando a eficácia remineralizadora em lesões de white-spot artificias da Caseína Fosfopeptídica- Fosfato de Cálcio Amorfo (CPP-ACP) e da resina infiltrante de esmalte (ICON®).
Materiais e Métodos: 13 espécimes de esmalte humano dividiram-se em 5 grupos.
Simularam-se lesões de cárie artificiais recorrendo à solução tampão de ácido lático, pH 5.0, durante 6 dias, em cada grupo. Adicionalmente ao: Grupo A (n=3) - aplicou-se, uma vez por dia, durante 4 semanas, CPP-ACP, alternando esta aplicação com a imersão das amostras em saliva artificial a 37ºC; Grupo B (n=3) - aplicou-se uma vez por dia, durante 4 semanas, CPP-ACP, sendo esta aplicação alternada com a imersão das amostras em saliva artificial. Posteriormente, aplicou-se resina infiltrante, ICON®; Grupo C (n=3) - as
amostras mantiveram-se em saliva artificial, durante 4 semanas, aplicando-se depois resina infiltrante, ICON®; Grupo D+ (n=2) - as amostras mantiveram-se imersas em saliva artificial, durante 4 semanas; Grupo D- (n=2) - as amostras mantiveram-se imersas em água desionizada, durante 4 semanas. Os espécimes foram submetidos a três radiovisiografias: antes/depois da indução de cárie e depois da remineralização. Analisou-se
a superfície do esmalte ao microscópio ótico, e fez-se uma análise descritiva e qualitativa das amostras.
Resultados: Os agentes utilizados nos grupos A, B e C apresentaram potencial para remineralizar as WSL. No grupo A e B os prismas de esmalte apresentaram-se homogéneos. No grupo C e D constatou-se uma heterogeneidade nos prismas do esmalte. Não se observaram diferenças significativas nas radiovisiografias.
Conclusões: Os grupos A e B, tratados com CPP-ACP, parecem apresentar maior homogeneidade na superfície do esmalte, no entanto, ao ser uma análise qualitativa não podemos aferir qual o melhor agente remineralizador. Conclui-se que o RVG não é útil para a deteção de achados radiotransparentes no esmalte, induzidos desta forma.
Objectives: To histologically, qualify the enamel surface when applying remineralizing agents and an infiltrating resin, comparing the remineralizing efficacy in artificial white-spot lesions of Phosphopeptide Casein-Amorphous Calcium Phosphate (CPP- ACP) and an infiltrating enamel resin (ICON®). Materials and Methods: 13 human enamel specimens were divided into 5 groups. Artificial caries lesions were simulated with lactic acid buffer solution, pH 5.0, for 6 days. Moreover in: Group A (n=3): CPP-ACP was applied once a day for 4 weeks, alternating this application with immersion of samples in artificial saliva at 37ºC. Group B (n=3): CPP-ACP was applied once a day for 4 weeks, this application was alternated with the immersion of samples in artificial saliva. Subsequently, infiltrating resin, ICON®, was applied. Group C (n=3): the samples were kept in artificial saliva for 4 weeks, after which time, infiltrating resin, ICON®, was applied. Group D+ (n=2): the samples were kept immersed in artificial saliva for 4 weeks. Group D- (n=2): the samples were kept immersed in deionized water for 4 weeks. All specimens from each group were submitted to three radiovisiographies: before and after caries induction and after remineralization. The enamel surface was analyzed under an optical microscope, where a qualitative and descriptive analysis of all samples was made. Results: All agents used in groups A, B and C showed the potential to remineralize WSL. In group A and B, the enamel prisms were homogeneous. In group C and D, enamel prisms were heterogeneous. No significant differences were observed in the radiovisiographs. Conclusions: Groups A and B, treated with CPP-ACP, seem to present greater homogeneity on the enamel surface, however, as it is a qualitative analysis, we cannot assess which is the best remineralizing agent. It is concluded that RVG is not useful for detecting radiolucent findings in enamel, treated this way.
Objectives: To histologically, qualify the enamel surface when applying remineralizing agents and an infiltrating resin, comparing the remineralizing efficacy in artificial white-spot lesions of Phosphopeptide Casein-Amorphous Calcium Phosphate (CPP- ACP) and an infiltrating enamel resin (ICON®). Materials and Methods: 13 human enamel specimens were divided into 5 groups. Artificial caries lesions were simulated with lactic acid buffer solution, pH 5.0, for 6 days. Moreover in: Group A (n=3): CPP-ACP was applied once a day for 4 weeks, alternating this application with immersion of samples in artificial saliva at 37ºC. Group B (n=3): CPP-ACP was applied once a day for 4 weeks, this application was alternated with the immersion of samples in artificial saliva. Subsequently, infiltrating resin, ICON®, was applied. Group C (n=3): the samples were kept in artificial saliva for 4 weeks, after which time, infiltrating resin, ICON®, was applied. Group D+ (n=2): the samples were kept immersed in artificial saliva for 4 weeks. Group D- (n=2): the samples were kept immersed in deionized water for 4 weeks. All specimens from each group were submitted to three radiovisiographies: before and after caries induction and after remineralization. The enamel surface was analyzed under an optical microscope, where a qualitative and descriptive analysis of all samples was made. Results: All agents used in groups A, B and C showed the potential to remineralize WSL. In group A and B, the enamel prisms were homogeneous. In group C and D, enamel prisms were heterogeneous. No significant differences were observed in the radiovisiographs. Conclusions: Groups A and B, treated with CPP-ACP, seem to present greater homogeneity on the enamel surface, however, as it is a qualitative analysis, we cannot assess which is the best remineralizing agent. It is concluded that RVG is not useful for detecting radiolucent findings in enamel, treated this way.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Resina infiltrante Fosfato de cálcio Remineralização Microscopia ótica