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Berenguer, Pedro Henrique Fernandes da Silva
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- Registo Oncológico na Região, 2018Publication . Camacho, Carolina; Queiroz Nunes, Carolina; Fraga, Cláudia; Santos, Filipa; Camacho, José; Barradas, Nuno; Serrão, Patrícia; Ornelas, Paula; Berenguer, Pedro; Rubina, Lara; Teixeira, Rubina; Câmara, Sara; Muller, SaraIntrodução : Em 2018, foram identificados na RAM 1410 novos casos de doenças oncológicas. Destes, 1159 corresponderam a casos de tumores malignos que foram incluídos na análise estatística principal. A exclusão de carcinomas da pele não melanoma (tipo basocelular, espinocelular, não especificados e outros) realiza-se de modo a ser possível a comparação com outros dados estatísticos nacionais e internacionais. A taxa de incidência bruta de cancro na RAM foi de 455,2/100.000 habitantes. Os cancros mais frequentes na RAM em 2018 foram o cancro da mama, da próstata, do cólon e do recto, da cabeça e pescoço (cavidade oral, faringe, vias aéreas superiores) e do pulmão.Objetivos e Metodologia O presente relatório constitui o segundo volume do Registo Oncológico da RAM, e contém informação sobre a incidência de cancro em 2018, na Região Autónoma da Madeira. Pretende -se que a publicação de dados do registo oncológico tenha uma periodicidade anual. Objetivos: 1. Recolher dos dados inseridos na plataforma do RON (Query), referente a novos casos de Tumores Malignos na RAM no ano de 2018, de forma a estabelecer parâmetros estatísticos legíveis e de fácil interpretação. 2. Aferir dados demográficos da população alvo, caracterização clínica e biológica dos Tumores Malignos, taxas de incidência e dados de sobrevivência populacional. 3. Divulgação dos resultados obtidos a todos os profissionais de saúde da RAM e público em geral, em formato digital ou em livro para consulta. Métodos: Os dados utilizados foram recolhidos através de Query na plataforma nacional do RON, e inclui todos os novos casos diagnosticados na RAM de 01-01-2018 a 31-12-2018. A classificação topográfica e morfológica dos tumores baseou-se na terceira edição da International Classification of Diseases for Oncology (ICD-O-3). As tabelas e gráficos estão organizados segundo a nomenclatura da International Classification of Diseases, 10a edição, 1990 (ICD-10). Foram excluídos da análise principal os carcinomas basocelulares (M8090/3-M8110/3), espinocelulares (M8051/3-M8084/3, M8120/3- M8131/3) e não especificados (M8000/3 e M8010/3) da pele, para permitir a comparação dos dados de incidência com outros registos de cancro internacionais que não recolhem sistematicamente este tipo de dados. Os tumores in situ (M----/2) e MGUS (Gamapatia Monoclonal de Significado Incerto) também foram excluídos da análise principal. Na avaliação de frequências estatísticas é considerada a mediana em detrimento da média por representar uma medida estatística mais robusta com ponto de rutura de 50%, não sendo tão distorcida por valores extremos (ex. idade mediana, tempo de espera mediano, etc.). São apresentadas as taxas de incidência de cancro brutas, especificas para a idade, segundo o sexo, e padronizadas (população Europeia e Mundial). Para o cálculo foi utilizada a estimativa oficial da população residente, fornecidas pelo Direção Regional de Estatística da Madeira. Os resultados foram expressos como taxa anual por 100000 pessoas-ano. Para permitir a comparação de taxas entre populações com diferentes estruturas etárias, as taxas foram padronizadas pelo método direto, com recurso à população padrão mundial e à população padrão europeia.
- Exploring the potential of a MEPS/UHPLC-based methodology on the Exploring the potential of a MEPS/UHPLC-based methodology on thePublication . Berenguer, Pedro; Camacho, Irene C; Câmara, José S.; Câmara, Rita; Oliveira, SusanaAsthma is a heterogeneous disease characterized by chronic inflammation and long term irreversible remodeling of the airways. The enzymatic peroxidation of the arachidonic acid is part of the pathophysiology of this disease and leads to the formation of powerful inflammatory mediators, characteristic of asthma. The present work aimed to develop an easy-to-use ultra-high pressure liquid chromatography (UHPLC)-based strategy in order to characterize lipid peroxidation biomarkers: leukotrienes E4 (LTE4) and B4 (LTB4) and 11β-prostaglandin F2α (11βPGF2α), eicosanoids present in the urine of asthmatic patients and healthy individuals (control group). A semi-automatic eVol®-microextraction by packed sorbent (MEPS) format was developed in order to isolate the target analytes. The method was fully validated under optimal extraction (R-AX sorbent, 3 conditioning-equilibration cycles with 250 μL of ACN-H2O at 0.1% FA, 10 extract-discard cycles of 250 μL of sample at a pH of 5.1, elution with 2 times 50 μL of MeOH and concentration of the eluate until half of its volume) and chromatographic conditions (14-min analysis at a flow rate of 300 μL min-1 in an UHPLCPDA equipped with a BEH C18 column). Our results indicated good recoveries (>95%) in addition to excellent extraction efficiency (>95%) at three concentration levels (low, mid and high) with precision (RSDs) less than 11%. The lack-of-fit, goodness-of-fit and Mandel’s fitting tests, revealed good linearity within the concentration range. Good selectivity and sensitivity were achieved with limits of detection ranging from 0.04 ng mL-1 for LTB4 to 1.12 ng mL-1 for 11βPGF2α, and limits of quantification from 0.10 ng mL- 1 for the LTB4 to 2.11 ng mL-1 for 11βPGF2α. The developed method was successfully applied to the urine of asthmatic patients and healthy individuals. On average, the urine of asthmatic patients present significantly higher concentrations of 11βPGF2α (112.96 ng mL-1 vs 62.56 ng mL-1 in control group), LTE4 (1.27 ng mL-1 vs 0.89 ng mL-1 in control group) and LTB4 (1.39 ng mL-1 vs 0.76 ng mL- 1 in control group). These results suggest the potential of the target eicosanoids and the developed method on asthma diagnosis and on the follow-up of the therapeutic response.
- P- CANCER AMONG ELDERLY:A 10-YEAR CHARACTERIZATION OF CASES FROM THE MADEIRA ARCHIPELAGO (PORTUGAL) CANCER REGISTRY.Publication . Berenguer, Pedro; Muller, Sara; Fraga, Cláudia; Serrão, Patrícia; Teixeira, Rubina; Camacho, José; Camacho, CarolinaThe evolution of science and improvement of medicine led to the extension of life expectancy resulting in a higher number of elder people among population.Consequently, life expectancy also increases diseases, such as cancer, one of the main causes of morbidity and mortality worldwide.In Portugal, cancer is the second leading cause of mortality, accounting for about 1/4 of deaths. With this work, it is intended to characterize cancer among elderly population, from Autonomous Region of Madeira (RAM), Portugal. The 3 most frequent cancer from each gender were addressed. etc.
- Determination of potential childhood asthma biomarkers using a powerful methodology based on microextraction by packed sorbent combined with ultra-high pressure liquid chromatography. Eicosanoids as case studyPublication . Berenguer, Pedro; Camacho, Irene C.; Câmara, Rita; Oliveira, Susana; Câmara, José S.Leukotrienes and prostaglandins are arachidonic acid bioactive derived eicosanoids and key mediators of bronchial inflammation and response modulation in the airways contributing to the pathophysiology of asthma. An easy-to-use ultra-high pressure liquid chromatography (UHPLC)-based strategy was developed to characterize biomarkers of lipid peroxidation: leukotrienes E (LTE4) and B4 (LTB4) and 11β-prostaglandin F2α (11βPGF2α), present in urine of asthmatic patients (N = 27) and healthy individuals (N = 17). A semi-automatic eVol®-microextraction by packed sorbent (MEPS) was used to isolate the target analytes. Several experimental parameters with influence on the extraction efficiency and on the chromatographic resolution, were evaluated and optimized. The method was fully validated under optimal extraction (R-AX sorbent, 3 conditioning-equilibration cycles with 250 μL of ACN-water at 0.1% FA, 10 extract-discard cycles of 250 μL of sample at a pH of 5.1, elution with 2 times 50 μL of MeOH and concentration of the eluate until half of its volume) and chromatographic conditions (14-min analysis at a flow rate of 300 μL min-1 in an UHPLC-PDA equipped with a BEH C18 column), according to IUPAC guidelines. The findings indicated good recoveries (>95%) in addition to excellent extraction efficiency (>95%) at three concentration levels (low mid and high) with precision (RSDs) less than 11%. The lack-of-fit test, goodness-of-fit test and Mandel's fitting test, revealed good linearity within the concentration range. Good selectivity and sensitivity were achieved with a limits of detection ranging from 0.04 μg L-1 for LTB4 to 1.12 μg L-1 for 11βPGF2α, and limits of quantification from 0.10 μg L-1 for the LTB4 to 2.11 μg L-1 for 11βPGF2α. The successful application of the fully validated method shows that, on average, the asthmatic patients had significantly higher concentrations of 11βPGF2α (112.96 μg L-1vs 62.56 μg L-1 in normal controls), LTE4 (1.27 μg L-1vs 0.89 μg L-1 in normal controls), and LTB4 (1.39 μg L-1vs 0.76 μg L-1 in normal controls). The results suggest the potential of the target eicosanoids on asthma diagnosis, however, a larger and more extensive study will be necessary to confirm the data obtained and to guarantee a greater robustness to the approach.
- P- Neoadjuvant Chemoradiotherapy as an Improved Prognostic Over rectal Cancer Patients . A 2-Decade study and its caracterizationPublication . Müller, Sara; Berenguer, Pedro; Fraga, Cláudia; Serrão, Patrícia; Rodrigues, Mariana; Teixeira, Rubina; Camacho, José; Camacho, Carolina