Percorrer por autor "Igrejas, Gilberto"
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- Study of InDel genetic markers with forensic and ancestry informative interest in PALOP's immigrant populations in LisboaPublication . Inácio, Ana; Costa, Heloísa Afonso; Silva, Cláudia Vieira da; Ribeiro, Teresa; Porto, Maria João; Santos, Jorge Costa; Igrejas, Gilberto; Amorim, AntónioThe migratory phenomenon in Portugal has become one of the main factors for the genetic variability. In the last few years, a new class of autosomal insertion/deletion markers—InDel—has attracted interest in forensic genetics. Since there is no data for InDel markers of Portuguese-speaking African countries (PALOP) immigrants living in Lisboa, our aim is the characterization of those groups of individuals by typing them with at least 30 InDel markers and to compare different groups of individuals/populations. We studied 454 bloodstain samples belonging to immigrant individuals from Angola, Guinea-Bissau, and Mozambique. DNA extraction was performed with the Chelex® 100 method. After extraction, all samples were typed with the Investigator® DIPplex method. Through the obtained results, allelic frequencies show that all markers are at Hardy-Weinberg equilibrium, and we can confirm that those populations show significant genetic distances between themselves, between them, and the host Lisboa population. Because of this, they introduce genetic variability in Lisboa population.
- Study of Indel genetic markers with forensic interest and ancestry informative power in PALOP’s immigrant populations in Lisboa - preliminary resultsPublication . Inácio, Ana; Afonso Costa, Heloísa; Vieira da Silva, Cláudia; Ribeiro, Teresa; Porto, Maria João; Costa Santos, Jorge; Igrejas, Gilberto; Amorim, AntónioThe increasing number of immigrants in Portugal is an unavoidable reality. According to Portugal Contemporary Base - PORDATA -, by the end of 2013, the foreign population living in Portugal was about 398.268, i.e., about 4% of the total population. The total number of immigrants from Portuguese-speaking African countries (PALOP) was about 100.800, from which 20.000 were from Angola, 42.000 from Cabo Verde, 176.600 from Guiné-Bissau, 2.850 from Moçambique and 10.170 from São Tomé e Príncipe. From those 100.800, about 75.000 are part of Lisboa population. The migratory phenomenon in Portugal can be one of the main factors for the genetic variability. In the last years, a new class of autosomal insertion/deletion markers - InDel -, has gained interest in forensic genetics. They are characterized by the presence or absence of a specific sequence of nucleotides. Significative differences in allele frequencies of InDel markers, between different groups or populations, can be used as evolutionary and ancestry indicators. Since there is few data for InDel markers of PALOP immigrants living in Lisboa, our aim is to characterize those groups of individuals by typing them with, at least, 30 markers and compare different groups of individuals/populations.
- Study of y-SNPs genetic markers with forensic interest and ancestry informative power in Palop’s immigrant populations in LisboaPublication . Inácio, Ana; Afonso Costa, Heloísa; da Silva, Cláudia; Ribeiro, Teresa; Porto, Maria João; Santos, Jorge; Igrejas, Gilberto; Amorim, AntónioSince the early 70's, the flow of immigrants from African countries to Portugal has increased. According to Portugal Contemporary Base - PORDATA -, by the end of 2013, the total number of immigrants from PALOP (Portuguese-speaking African countries) in Portugal was about 100 000, and from those, 75 000 are part of Lisboa population. The number of immigrants in Portugal is an unavoidable reality and the migratory phenomenon in this country and, particularly in Lisboa, can become one of the main factors for the genetic variability The single nucleotide polymorphisms (SNP) typically involve substitution of a nucleotide in DNA sequence, resulting an exchange on the sequence. SNP in forensic genetics are mainly used in samples where DNA is degraded, since only a small target DNA region is necessary because the size of the amplified product is under 100 bp. Recent advances in the SNP markers, show us the growing interest of Forensic Genetics in their use. Markers located on the Y chromosome have special interest and application in origin and evolution population studies. Each chromosome has the story of millions of years of evolution and Y chromosome is no exception, telling the story of a male lineage. Having diverged from the same ancestral that the Xchromosome, the Y chromosome passed from generation to generation without change, except for the occurrence of mutations. Y-SNPs are single nucleotide polymorphisms, with ancestry and population applications, and also with forensic application. Since there is no data for Y-SNPs markers of PALOP immigrants living in Lisboa, our aim is the characterization of those groups of individuals by typing them with a panel of Y-SNPs proposed by Rosser and collaborators in 2000, with 9 YSNPs markers, and compare different groups of individuals/populations.