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Gelatin-based membrane for enhanced periodontal tissue regeneration
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Esta dissertação desenvolveu hidrogéis de gelatina-policaprolactona funcionalizados com nanopartículas inorgânicas para potenciar a regeneração periodontal. Foram sintetizadas nanopartículas de hidroxiapatite, fosfato tricálcico com substituições de ferro e carbonato de cálcio, cuja caracterização confirmou elevada cristalinidade e morfologias nanométricas distintas. Ensaios biológicos demonstraram boa citocompatibilidade para todas as partículas, evidenciando atividade osteogénica através do aumento da atividade de fosfatase alcalina em culturas de linha celular MG-63, células de osteossarcoma humano semelhantes a fibroblastos. A incorporação das nanopartículas nos hidrogéis originou estruturas com diferentes perfis de degradação e modificações nas propriedades reológicas. As análises de gelificação revelaram intervalos térmicos adequados para impressão 3D por extrusão, permitindo produzir membranas homogéneas e estáveis. Entre as composições estudadas, o hidrogel contendo carbonato de cálcio apresentou a maior estabilidade in vitro, menor taxa de degradação, mantendo uma boa imprimibilidade. Estes resultados demonstram o seu potencial como biomaterial personalizável para regeneração periodontal, promovendo condições favoráveis à formação de novo tecido ósseo.
This dissertation developed gelatin–polycaprolactone hydrogels functionalized with inorganic nanoparticles to enhance periodontal regeneration. Hydroxyapatite nanoparticles, ironsubstituted tricalcium phosphate, and calcium carbonate were synthesized, and their characterization confirmed high crystallinity and distinct nanoscale morphologies. Biological assays demonstrated good cytocompatibility for all particles, revealing osteogenic activity through increased alkaline phosphatase activity in MG-63 cultures, a human osteosarcoma cell line with fibroblast-like characteristics. Incorporating the nanoparticles into the hydrogels yielded structures with varying degradation profiles and alterations in rheological properties. Gelation analyses indicated thermal windows suitable for extrusion-based 3D printing, enabling the production of homogeneous and stable membranes. Among the compositions investigated, the hydrogel containing calcium carbonate showed the greatest in vitro stability and the lowest degradation rate while maintaining good printability. These results highlight its potential as a customizable biomaterial for periodontal regeneration, fostering conditions conducive to new bone tissue formation.
This dissertation developed gelatin–polycaprolactone hydrogels functionalized with inorganic nanoparticles to enhance periodontal regeneration. Hydroxyapatite nanoparticles, ironsubstituted tricalcium phosphate, and calcium carbonate were synthesized, and their characterization confirmed high crystallinity and distinct nanoscale morphologies. Biological assays demonstrated good cytocompatibility for all particles, revealing osteogenic activity through increased alkaline phosphatase activity in MG-63 cultures, a human osteosarcoma cell line with fibroblast-like characteristics. Incorporating the nanoparticles into the hydrogels yielded structures with varying degradation profiles and alterations in rheological properties. Gelation analyses indicated thermal windows suitable for extrusion-based 3D printing, enabling the production of homogeneous and stable membranes. Among the compositions investigated, the hydrogel containing calcium carbonate showed the greatest in vitro stability and the lowest degradation rate while maintaining good printability. These results highlight its potential as a customizable biomaterial for periodontal regeneration, fostering conditions conducive to new bone tissue formation.
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Keywords
Hidrogel Funcionalização Nanopartículas Impressão 3D Regeneração do tecido ósseo Aplicações periodontais Hydrogel Functionalization Nanoparticles 3D printing Bone tissue regeneration Periodontal applications