Publication
In vivo clonal analysis reveals random monoallelic expression in lymphocytes that traces back to hematopoietic stem cells
| datacite.subject.fos | Ciências Médicas | |
| datacite.subject.sdg | 03:Saúde de Qualidade | |
| dc.contributor.author | Kubasova, Nadiya | |
| dc.contributor.author | Alves-Pereira, Clara F. | |
| dc.contributor.author | Gupta, Saumya | |
| dc.contributor.author | Vinogradova, Svetlana | |
| dc.contributor.author | Gimelbrant, Alexander | |
| dc.contributor.author | Barreto, Vasco M. | |
| dc.date.accessioned | 2025-11-17T12:17:34Z | |
| dc.date.available | 2025-11-17T12:17:34Z | |
| dc.date.issued | 2022-08 | |
| dc.description.abstract | Evaluating the epigenetic landscape in the stem cell compartment at the single-cell level is essential to assess the cells’ heterogeneity and predict their fate. Here, using a genome-wide transcriptomics approach in vivo, we evaluated the allelic expression imbalance in the progeny of single hematopoietic cells (HSCs) as a read-out of epigenetic marking. After 4 months of extensive proliferation and differentiation, we found that X-chromosome inactivation (XCI) is tightly maintained in all single-HSC derived hematopoietic cells. In contrast, the vast majority of the autosomal genes did not show clonal patterns of random monoallelic expression (RME). However, a persistent allele-specific autosomal transcription in HSCs and their progeny was found in a rare number of cases, none of which has been previously reported. These data show that: 1) XCI and RME in the autosomal chromosomes are driven by different mechanisms; 2) the previously reported high frequency of genes under RME in clones expanded in vitro (up to 15%) is not found in clones undergoing multiple differentiation steps in vivo; 3) prior to differentiation, HSCs have stable patterns of autosomal RME. We propose that most RME patterns in autosomal chromosomes are erased and established de novo during cell lineage differentiation. | eng |
| dc.identifier.citation | Kubasova N, Alves-Pereira CF, Gupta S, Vinogradova S, Gimelbrant A and Barreto VM (2022) In Vivo Clonal Analysis Reveals Random Monoallelic Expression in Lymphocytes That Traces Back to Hematopoietic Stem Cells. Front. Cell Dev. Biol. 10:827774. doi: 10.3389/fcell.2022.827774 | |
| dc.identifier.doi | 10.3389/fcell.2022.827774 | |
| dc.identifier.issn | 2296-634X | |
| dc.identifier.uri | http://hdl.handle.net/10400.26/59812 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Frontiers Media | |
| dc.relation.hasversion | https://doi.org/10.3389/fcell.2022.827774 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | allele-specific expression | |
| dc.subject | random monoallelic expression (RME) | |
| dc.subject | allelic imbalance (AI) | |
| dc.subject | epigenetics | |
| dc.subject | clonal analysis | |
| dc.subject | hematopoietic stem cell (HSC) | |
| dc.subject | X-chromosome inactivation (XCI) | |
| dc.subject | RNA-seq | |
| dc.title | In vivo clonal analysis reveals random monoallelic expression in lymphocytes that traces back to hematopoietic stem cells | eng |
| dc.type | contribution to journal | |
| dspace.entity.type | Publication | |
| oaire.citation.startPage | 827774 | |
| oaire.citation.title | Frontiers in Cell and Developmental Biology | |
| oaire.citation.volume | 10 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |