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In vivo clonal analysis reveals random monoallelic expression in lymphocytes that traces back to hematopoietic stem cells
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Orientador(es)
Resumo(s)
Evaluating the epigenetic landscape in the stem cell compartment at the single-cell level is essential to assess the cells’ heterogeneity and predict their fate. Here, using a genome-wide transcriptomics approach in vivo, we evaluated the allelic expression imbalance in the progeny of single hematopoietic cells (HSCs) as a read-out of epigenetic marking. After 4 months of extensive proliferation and differentiation, we found that X-chromosome inactivation (XCI) is tightly maintained in all single-HSC derived hematopoietic cells. In contrast, the vast majority of the autosomal genes did not show clonal patterns of random monoallelic expression (RME). However, a persistent allele-specific autosomal transcription in HSCs and their progeny was found in a rare number of cases, none of which has been previously reported. These data show that: 1) XCI and RME in the autosomal chromosomes are driven by different mechanisms; 2) the previously reported high frequency of genes under RME in clones expanded in vitro (up to 15%) is not found in clones undergoing multiple differentiation steps in vivo; 3) prior to differentiation, HSCs have stable patterns of autosomal RME. We propose that most RME patterns in autosomal chromosomes are erased and established de novo during cell lineage differentiation.
Descrição
Palavras-chave
allele-specific expression random monoallelic expression (RME) allelic imbalance (AI) epigenetics clonal analysis hematopoietic stem cell (HSC) X-chromosome inactivation (XCI) RNA-seq
Contexto Educativo
Citação
Kubasova N, Alves-Pereira CF, Gupta S, Vinogradova S, Gimelbrant A and Barreto VM (2022) In Vivo Clonal Analysis Reveals Random Monoallelic Expression in Lymphocytes That Traces Back to Hematopoietic Stem Cells. Front. Cell Dev. Biol. 10:827774. doi: 10.3389/fcell.2022.827774
Editora
Frontiers Media