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Alterações físico-químicas dos cones de guta-percha após o protocolo de desinfeção rápida e sua relevância clínica
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Abstract(s)
Objetivo: O presente estudo visa avaliar e comparar a composição química de 5 marcas de cones de guta-percha padronizados nº25 (Cerkamed, ProTaper Gold, Reciproc, Autofit e Zarc) e as possíveis alterações em cada marca após o protocolo de desinfeção rápida. Materiais e Métodos: Análise da composição química: dissolução dos cones de guta-percha em clorofórmio e posterior centrifugação, ocorrendo a formação de duas fases deste material, fase inorgânica e orgânica. Análise WDXRF: termoplastificação dos cones, compressão a 10t e análise em vácuo. Análise da radiopacidade: análise digital da transição entre o terço apical e o terço médio, com o software Gimp 2.0 e um stepwedge. Análise da atividade antimicrobiana: inoculação em meio de agar de Muller Hinton, de Enterococcus feacalis, e de Staphylococcus aureus colocação dos cones de guta-percha de cada uma das marcas no agar e observação da possibilidade de formação de halos. Os cones de guta-percha foram divididos em 3 grupos: Grupo C sem tratamento, Grupo S imersão (NaClO 5,25%) e Grupo A imersão (NaClO 5,25%) e lavagem com álcool (96%). Resultados: Análise da composição química: fração orgânica apresentou percentagens entre 13,4-21,3%, guta-percha, 11,5-18,8%, ceras e resinas, 1,2-2,5% e fração inorgânica, 77,3-85,6%. Análise WDXRF: os óxidos identificados foram: ZnO, BaSO4, Na2O, TiO2, MgO, SiO2. Análise da radiopacidade: diferentes níveis de radiopacidade entre marcas: Cerkamed mais baixa e Zarc mais elevada, sendo que Autofit e ProTaper Gold revelam radiopacidades semelhantes. Não foram registadas propriedades antimicrobianas. Conclusões: As análises de composição química, da técnica WDXRF e da radiopacidade demonstram que existem diferenças significativas entre as 5 marcas antes do protocolo de desinfeção rápida e que Zn poderá ter influência na radiopacidade. Nenhuma marca de cones de guta-percha revelou possuir atividade antimicrobiana. O protocolo de desinfeção rápida não provoca nenhum tipo de alteração relativamente às características investigadas.
Objective: The present study aims to evaluate and compare the chemical composition of 5 brands of gutta-percha points, n25, (Cerkamed, ProTaper Gold, Reciproc, Autofit e Zarc) and the possible changes after a quick disinfection protocol. Materials and Methods: Chemical composition analysis: gutta-percha points were dissolved in chloroform and centrifuged, leading to the formation of two phases, an inorganic and organic phase. WDXRF analysis: thermo-plastification of the material was performed, followed by compression at 10 t and analysis was performed in vacuum. Radiopacity analysis: Digital radiographs of the transition between the medial and apex thirds were obtained and analysed with the software Gimp 2.0 and stepwedge. Antimicrobial activity analysis: Inoculation of Enterococcus feacalis and Staphylococcus aureus in Muller Hinton agar medium, followed by placement of the gutta-percha points of each brand in the agar. These were observed for halo formation. The points were divided into 3 groups: Group C no treatment, Group S immersion (5,25% NaClO) and Group A immersion (5,25% NaClO) followed by rinsing in alcohol (96%). Results: Chemical composition analysis show: the organic phase was 13,4-21,3%, gutta-percha 11,5-18,8%, resin and waxes 1,3-2,5% and the inorganic phase 77,3-85,6%. WDXRF analysis, identified several oxides like: ZnO, BaSO4, Na2O, TiO2, MgO, SiO2. Radiopacity analysis shows, different levels of radiopacity: Cerkamed exhibiting the lowest, Zarc the highest, while Autofit and ProTaper Gold showed similar radiopacity. Antimicrobial activity analysis demonstrated no antimicrobial activity. Conclusion: The chemical composition analysis, WDXRF and radiopacity analysis showed that there are statistically significant differences between the 5 brands investigated here, before they were submitted to a quick disinfection protocol and that Zn can influence the radiopacity. It should be noted that none of the investigated brands possessed antimicrobial activity. Employment of a quick disinfection protocol doesn’t cause any kind of change related to the characteristics investigated.
Objective: The present study aims to evaluate and compare the chemical composition of 5 brands of gutta-percha points, n25, (Cerkamed, ProTaper Gold, Reciproc, Autofit e Zarc) and the possible changes after a quick disinfection protocol. Materials and Methods: Chemical composition analysis: gutta-percha points were dissolved in chloroform and centrifuged, leading to the formation of two phases, an inorganic and organic phase. WDXRF analysis: thermo-plastification of the material was performed, followed by compression at 10 t and analysis was performed in vacuum. Radiopacity analysis: Digital radiographs of the transition between the medial and apex thirds were obtained and analysed with the software Gimp 2.0 and stepwedge. Antimicrobial activity analysis: Inoculation of Enterococcus feacalis and Staphylococcus aureus in Muller Hinton agar medium, followed by placement of the gutta-percha points of each brand in the agar. These were observed for halo formation. The points were divided into 3 groups: Group C no treatment, Group S immersion (5,25% NaClO) and Group A immersion (5,25% NaClO) followed by rinsing in alcohol (96%). Results: Chemical composition analysis show: the organic phase was 13,4-21,3%, gutta-percha 11,5-18,8%, resin and waxes 1,3-2,5% and the inorganic phase 77,3-85,6%. WDXRF analysis, identified several oxides like: ZnO, BaSO4, Na2O, TiO2, MgO, SiO2. Radiopacity analysis shows, different levels of radiopacity: Cerkamed exhibiting the lowest, Zarc the highest, while Autofit and ProTaper Gold showed similar radiopacity. Antimicrobial activity analysis demonstrated no antimicrobial activity. Conclusion: The chemical composition analysis, WDXRF and radiopacity analysis showed that there are statistically significant differences between the 5 brands investigated here, before they were submitted to a quick disinfection protocol and that Zn can influence the radiopacity. It should be noted that none of the investigated brands possessed antimicrobial activity. Employment of a quick disinfection protocol doesn’t cause any kind of change related to the characteristics investigated.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Guta-percha Hipoclorito de sódio Composição química