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Studying genetic damage and repair using 3D breast cancer cell models
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Resumo(s)
O cancro da mama continua a ser uma das principais causas de morte entre mulheres em todo o mundo. O objetivo desde estudo foi avaliar os efeitos do peróxido de hidrogénio (H2O2) e da doxorrubicina (DOX) em três linhas celulares mamárias – MCF-10A
(controlo), MCF-7 e MDA-MB-231 – cultivadas em duas e três dimensões (esferóides). A lesão no DNA foi avaliado através do ensaio Cometa e foi também realizada uma análise morfológica. Ambos os agentes induziram lesão significativa no DNA, com maior
sensibilidade observada nas culturas 2D. Os esferóides apresentaram maior resistência, destacando a importância da dimensionalidade na resposta a fármacos e ao stress oxidativo na investigação do cancro da mama.
Breast cancer (BC) remains one of the leading causes of death among women worldwide. The aim of this study was to evaluate the effects of hydrogen peroxide (H2O2) and doxorubicin (DOX) in three BC cell lines – MCF-10A (control), MCF-7 and MDA-MB 231 – both in two-dimensional (2D) and three-dimensional (3D) cultures (spheroids). DNA damage was assessed using the Comet Assay and a morphological analysis was also performed. Both agents induced significant DNA damage, with higher sensitivity observed in 2D cultures. The 3D spheroids presented increased resistance, highlighting the importance of dimensionality, in both drug response and oxidative stress in BC research.
Breast cancer (BC) remains one of the leading causes of death among women worldwide. The aim of this study was to evaluate the effects of hydrogen peroxide (H2O2) and doxorubicin (DOX) in three BC cell lines – MCF-10A (control), MCF-7 and MDA-MB 231 – both in two-dimensional (2D) and three-dimensional (3D) cultures (spheroids). DNA damage was assessed using the Comet Assay and a morphological analysis was also performed. Both agents induced significant DNA damage, with higher sensitivity observed in 2D cultures. The 3D spheroids presented increased resistance, highlighting the importance of dimensionality, in both drug response and oxidative stress in BC research.
Descrição
Palavras-chave
Cancro da mama Peróxido de Hidrogénio Doxorrubicina Esferóides Dano no DNA Ensaio Cometa Breast cancer Hydrogen Peroxide Doxorubicin Spheroids DNA damage Comet Assay