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Genomic epidemiological analysis of Klebsiella pneumoniae from Portuguese hospitals reveals insights into circulating antimicrobial resistance

2022-08Contribution to journal Open access
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datacite.subject.fosCiências Médicas
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorSpadar, Anton
dc.contributor.authorPhelan, Jody
dc.contributor.authorElias, Rita
dc.contributor.authorModesto, Ana
dc.contributor.authorCaneiras, Cátia
dc.contributor.authorMarques, Cátia
dc.contributor.authorLito, Luís
dc.contributor.authorPinto, Margarida
dc.contributor.authorCavaco-Silva, Patrícia
dc.contributor.authorFerreira, Helena
dc.contributor.authorPomba, Constança
dc.contributor.authorSilva, Gabriela J. Da
dc.contributor.authorSaavedra, Maria José
dc.contributor.authorMelo-Cristino, José
dc.contributor.authorDuarte, Aida
dc.contributor.authorCampino, Susana
dc.contributor.authorPerdigão, João
dc.contributor.authorClark, Taane G.
dc.date.accessioned2025-11-13T10:11:58Z
dc.date.available2025-11-13T10:11:58Z
dc.date.issued2022-08
dc.description.abstractKlebsiella pneumoniae (Kp) bacteria are an increasing threat to public health and represent one of the most concerning pathogens involved in life-threatening infections and antimicrobial resistance (AMR). To understand the epidemiology of AMR of Kp in Portugal, we analysed whole genome sequencing, susceptibility testing and other meta data on 509 isolates collected nationwide from 16 hospitals and environmental settings between years 1980 and 2019. Predominant sequence types (STs) included ST15 (n = 161, 32%), ST147 (n = 36, 7%), ST14 (n = 26, 5%) or ST13 (n = 26, 5%), while 31% of isolates belonged to STs with fewer than 10 isolates. AMR testing revealed widespread resistance to aminoglycosides, fluoroquinolones, cephalosporins and carbapenems. The most common carbapenemase gene was blaKPC-3. Whilst the distribution of AMR linked plasmids appears uncorrelated with ST, their frequency has changed over time. Before year 2010, the dominant plasmid group was associated with the extended spectrum beta-lactamase gene blaCTX-M-15, but this group appears to have been displaced by another carrying the blaKPC-3 gene. Co-carriage of blaCTX-M and blaKPC-3 was uncommon. Our results from the largest genomics study of Kp in Portugal highlight the active transmission of strains with AMR genes and provide a baseline set of variants for future resistance monitoring and epidemiological studies.eng
dc.identifier.citationSpadar, A., Phelan, J., Elias, R. et al. Genomic epidemiological analysis of Klebsiella pneumoniae from Portuguese hospitals reveals insights into circulating antimicrobial resistance. Sci Rep 12, 13791 (2022). https://doi.org/10.1038/s41598-022-17996-1
dc.identifier.doi10.1038/s41598-022-17996-1
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.26/59709
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Nature
dc.relation.hasversionhttps://doi.org/10.1038/s41598-022-17996-1
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAntibiotics
dc.subjectAntimicrobial resistance
dc.subjectBacterial genomics
dc.titleGenomic epidemiological analysis of Klebsiella pneumoniae from Portuguese hospitals reveals insights into circulating antimicrobial resistanceeng
dc.typecontribution to journal
dspace.entity.typePublication
oaire.citation.startPage13791
oaire.citation.titleScientific Reports
oaire.citation.volume12
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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