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Quantificação das diferentes subpopulações de células B e plasmoblastos do sangue periférico de transplantados renais
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Abstract(s)
O transplante de rim é a opção terapêutica e de reabilitação preferencial para a maioria dos doentes com insuficiência renal crónica terminal. Uma das principais complicações da transplantação renal é a ocorrência de rejeição e/ou disfunção crónica do enxerto.
Se durante muitos anos o principal alvo para a indução de tolerância foram os linfócitos T, hoje em dia, reconhece-se que as células B desempenham um importante papel no sucesso da transplantação.
Foi objectivo deste trabalho quantificar por citometria de fluxo as diferentes subpopulações de células B e de plasmoblastos no sangue periférico de doentes transplantados renais, assim como a expressão de imunoglobulinas de superfície nas células B memória e plasmoblastos. Diferenças significativas no número de linfócitos B totais e suas subpopulações do sangue periférico foram encontradas, quer entre os distintos grupos de transplantados renais estudados, quer comparativamente ao grupo controlo. Tais alterações numéricas podem ser características de doentes transplantados renais, reflectindo a acção da terapêutica imunossupressora realizada, e reforçam o papel importante destas células na progressão da situação clínica face ao enxerto.
Organ transplantation is the main therapy for most patients with end stage renal disease with the particularity of induces a specific tolerance in the recipient. For many years, the predominant focus of research was to induce tolerance on T cells. Today we know that B cells play an important role in transplantation. In the present paper we evaluated by flow citometry the numbers of peripheral blood B cells and distinct B cell subsets, as well as the expression of surface immunoglobulins in memory B cells and plasmablasts from renal transplant patients. Generally, significant differences in the number of total B lymphocytes and their subpopulations were found either between the distinct groups of patients or the control group. These numeric alterations can be seen as a feature of renal transplant patients, reflecting the action of immunosuppressive therapy, and highlighting the relevant role of these cells in the clinical progression.
Organ transplantation is the main therapy for most patients with end stage renal disease with the particularity of induces a specific tolerance in the recipient. For many years, the predominant focus of research was to induce tolerance on T cells. Today we know that B cells play an important role in transplantation. In the present paper we evaluated by flow citometry the numbers of peripheral blood B cells and distinct B cell subsets, as well as the expression of surface immunoglobulins in memory B cells and plasmablasts from renal transplant patients. Generally, significant differences in the number of total B lymphocytes and their subpopulations were found either between the distinct groups of patients or the control group. These numeric alterations can be seen as a feature of renal transplant patients, reflecting the action of immunosuppressive therapy, and highlighting the relevant role of these cells in the clinical progression.
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Keywords
Transplante renal Linfócitos B Plasmoblastos Imunoglobulinas Kidney transplant B-Lymphocytes Plasmoblasts Immunoglobulins