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Avaliação da nanoinfiltração em dentina de um sistema adesivo universal experimental sem Bis-GMA
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Advisor(s)
Abstract(s)
Objetivos: Avaliar a nanoinfiltração na dentina de dois sistemas adesivos universais
experimentais, e de dois adesivos universais comerciais, nas vertentes Etch-and-Rinse e
Self-Etch.
Materiais e Métodos: Foram utilizados 24 dentes molares hígidos divididos
aleatoriamente em 8 grupos (n=3): Em 4 grupos foram utilizados dois sistemas adesivos
comerciais – FUT (Futurabond M+TM) e SBU (ScotchbondTM) e os restantes 4 grupos em dois sistemas adesivos experimentais – EM1 (com Bis-GMA) e EM2 (sem Bis-GMA),
nas vertentes Etch-and-Rinse e Self-etch. A superfície da dentina foi exposta, os sistemas adesivos foram aplicados segundo as instruções do fabricante e foram construídos blocos de resina composta de 6-8mm de altura. As amostras foram cortadas em fatias de 0,7mm de espessura num micrótomo de tecido duros sendo para cada grupo selecionadas as 3 fatias centrais. Todos os espécimes foram incubados numa estufa a 37ºC durante três meses em água destilada. Findo este período, as amostras foram preparadas para a observação da nanoinfiltração por SEM-EDS sendo desidratadas e fixadas com nitrato de prata. A análise estatística da nanoinfiltração foi efetuada em SPSS através de modelos lineares mistos.
Resultados: Existem diferenças significativas na nanoinfiltração entre os adesivos
estudados e em função do protocolo aplicado (Etch-and-Rinse e Self- Etch). O EM1
apresenta menor nanoinfiltração quando comparado com FUT (p<0,001) e SBU
(p=0,002) sob qualquer protocolo. O EM2 apresenta menor nanoinfiltração quando
comparado com FUT e SBU sobre o protocolo Etch-and-Rinse (p <0,001). O EM1
apresenta menor nanoinfiltração quando comparado com EM2 apenas sob protocolo Self- Etch (p<0,001).
Conclusões: Os sistemas adesivos experimentais em estudo revelaram resultados
promissores comparativamente com os sistemas adesivos comerciais estudados. Após 3
meses de envelhecimento, o EM1 revelou a menor taxa de nanoinfiltração em ambos os
protocolos (Etch-and-Rinse e Self-Etch) e sem diferenças para o EM2 apenas quando
aplicado com o protocolo Etch-and-Rinse.
Objectives: To evaluate the dentin nanoleakage of two experimental universal adhesive systems and two commercial universal adhesives in the Etch-and-Rinse and Self-Etch protocol. Materials and Methods: 24 healthy molar teeth were randomly divided into 8 groups (n = 3): 4 groups were used two commercial adhesive systems - FUT (Futurabond M + TM) and SBU (ScotchbondTM) and the remaining 4 groups were used two experimental adhesive systems - EM1 (with Bis-GMA) and EM2 (without Bis-GMA), in the Etch-and- Rinse and Self-Etch strands. The dentin surface was exposed, the adhesive systems were applied according to the manufacturer's instructions and blocks of 6-8mm height composite resin were built. The samples were cut into 0.7mm thick slices in a hard tissue microtome and the 3 central slices were selected for each group. All specimens were incubated in an oven at 37ºC for three months in distilled water. At the end of this period, the samples were prepared for the observation of nanoleakage by SEM-EDS and were dehydrated and fixed with silver nitrate. The statistical analysis of nanoleakage was carried out in SPSS using mixed linear models. Results: There are significant differences in nanoleakage between the studied adhesives according to the applied protocol (Etch-and-Rinse and Self-Etch). EM1 has less nanoleakage when compared to FUT (p <0.001) and SBU (p = 0.002) under any protocol. EM2 shows less nanoleakage when compared to FUT and SBU on the Etch-and-Rinse protocol (p <0.001). EM1 presents less nanoleakage when compared with EM2 only under the Self-Etch protocol (p <0.001). Conclusions: The experimental adhesive systems under study showed promising results compared to the commercial adhesive systems studied. After 3 months of aging, EM1 revealed the lowest rate of nanoleakage in both protocols (Etch-and-Rinse and Self-Etch) and with no differences for EM2 only when applied with the Etch-and-Rinse protocol.
Objectives: To evaluate the dentin nanoleakage of two experimental universal adhesive systems and two commercial universal adhesives in the Etch-and-Rinse and Self-Etch protocol. Materials and Methods: 24 healthy molar teeth were randomly divided into 8 groups (n = 3): 4 groups were used two commercial adhesive systems - FUT (Futurabond M + TM) and SBU (ScotchbondTM) and the remaining 4 groups were used two experimental adhesive systems - EM1 (with Bis-GMA) and EM2 (without Bis-GMA), in the Etch-and- Rinse and Self-Etch strands. The dentin surface was exposed, the adhesive systems were applied according to the manufacturer's instructions and blocks of 6-8mm height composite resin were built. The samples were cut into 0.7mm thick slices in a hard tissue microtome and the 3 central slices were selected for each group. All specimens were incubated in an oven at 37ºC for three months in distilled water. At the end of this period, the samples were prepared for the observation of nanoleakage by SEM-EDS and were dehydrated and fixed with silver nitrate. The statistical analysis of nanoleakage was carried out in SPSS using mixed linear models. Results: There are significant differences in nanoleakage between the studied adhesives according to the applied protocol (Etch-and-Rinse and Self-Etch). EM1 has less nanoleakage when compared to FUT (p <0.001) and SBU (p = 0.002) under any protocol. EM2 shows less nanoleakage when compared to FUT and SBU on the Etch-and-Rinse protocol (p <0.001). EM1 presents less nanoleakage when compared with EM2 only under the Self-Etch protocol (p <0.001). Conclusions: The experimental adhesive systems under study showed promising results compared to the commercial adhesive systems studied. After 3 months of aging, EM1 revealed the lowest rate of nanoleakage in both protocols (Etch-and-Rinse and Self-Etch) and with no differences for EM2 only when applied with the Etch-and-Rinse protocol.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Etch-and-Rinse Nanoinfiltração Self-Etch Sistemas adesivos universais