A Comparison Among Three Multiplex Y-STR Profiling Kits for Sexual Assault Cases

Ferreira-Silva, Benedita; Fonseca-Cardoso, Margarida; Porto, Maria João; Magalhães, Teresa; Cainé, Laura

http://hdl.handle.net/10400.26/43880

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Authors

Ferreira-Silva, Benedita

Fonseca-Cardoso, Margarida

Porto, Maria João

Magalhães, Teresa

Cainé, Laura

Abstract(s)

Biological evidence of sexual assault is one of the most difficult sample types to analyze in forensic laboratories. Y-STR markers are thus a valuable tool for analyzing these samples. The aim of this project was to compare three Y-STR commercial kits by analyzing their amplification performance on casework samples. Overall, 247 trace samples were analyzed with a Yfiler® Plus PCR Amplification Kit (Applied Biosystems), PowerPlex® Y23 (Promega® ) System and AmpFLSTR® Yfiler™ PCR Amplification Kit (Applied Biosystems). Comparing the amplification performance of the three kits, the first two were significantly more sensitive than the latter (p < 0.001). For samples, with a male DNA quantity less than 0.5 ng, the PowerPlex Y23® kit was the most sensitive and best performing kit, followed by the Yfiler® Plus kit (p = 0.009). In conclusion, the Yfiler® Plus and PowerPlex Y-23® kits are viable alternatives to older kits for samples with low amounts of male DNA.

Keywords

DNA typing Forensic genetics Sexual assault

URI

http://hdl.handle.net/10400.26/43880

Citation

Ferreira-Silva, B., Fonseca-Cardoso, M., Porto, M. J., Magalhães, T., & Cainé, L. (2018). A Comparison Among Three Multiplex Y-STR Profiling Kits for Sexual Assault Cases. Journal of forensic sciences, 63(6), 1836–1840.