Publication
A Comparison Among Three Multiplex Y-STR Profiling Kits for Sexual Assault Cases
| dc.contributor.author | Ferreira-Silva, Benedita | |
| dc.contributor.author | Fonseca-Cardoso, Margarida | |
| dc.contributor.author | Porto, Maria João | |
| dc.contributor.author | Magalhães, Teresa | |
| dc.contributor.author | Cainé, Laura | |
| dc.date.accessioned | 2023-02-16T15:22:06Z | |
| dc.date.available | 2023-02-16T15:22:06Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | Biological evidence of sexual assault is one of the most difficult sample types to analyze in forensic laboratories. Y-STR markers are thus a valuable tool for analyzing these samples. The aim of this project was to compare three Y-STR commercial kits by analyzing their amplification performance on casework samples. Overall, 247 trace samples were analyzed with a Yfiler® Plus PCR Amplification Kit (Applied Biosystems), PowerPlex® Y23 (Promega® ) System and AmpFLSTR® Yfiler™ PCR Amplification Kit (Applied Biosystems). Comparing the amplification performance of the three kits, the first two were significantly more sensitive than the latter (p < 0.001). For samples, with a male DNA quantity less than 0.5 ng, the PowerPlex Y23® kit was the most sensitive and best performing kit, followed by the Yfiler® Plus kit (p = 0.009). In conclusion, the Yfiler® Plus and PowerPlex Y-23® kits are viable alternatives to older kits for samples with low amounts of male DNA. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Ferreira-Silva, B., Fonseca-Cardoso, M., Porto, M. J., Magalhães, T., & Cainé, L. (2018). A Comparison Among Three Multiplex Y-STR Profiling Kits for Sexual Assault Cases. Journal of forensic sciences, 63(6), 1836–1840. | pt_PT |
| dc.identifier.doi | 10.1111/1556-4029.13757 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.26/43880 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Wiley | pt_PT |
| dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/10.1111/1556-4029.13757 | pt_PT |
| dc.subject | DNA typing | pt_PT |
| dc.subject | Forensic genetics | pt_PT |
| dc.subject | Sexual assault | pt_PT |
| dc.title | A Comparison Among Three Multiplex Y-STR Profiling Kits for Sexual Assault Cases | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 1840 | pt_PT |
| oaire.citation.startPage | 1836 | pt_PT |
| oaire.citation.title | Journal of Forensic Sciences | pt_PT |
| oaire.citation.volume | 63 (6) | pt_PT |
| person.familyName | Cainé | |
| person.givenName | Laura | |
| person.identifier | AAR-9087-2020 | |
| person.identifier.ciencia-id | 021A-33DD-869D | |
| person.identifier.orcid | 0000-0002-6542-4256 | |
| person.identifier.scopus-author-id | 2311978 | |
| rcaap.rights | closedAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 1a1e1ffc-1439-48f2-a4c3-359c66db3ad1 | |
| relation.isAuthorOfPublication.latestForDiscovery | 1a1e1ffc-1439-48f2-a4c3-359c66db3ad1 |