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Análise da mineralização dentinária in vitro
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Advisor(s)
Abstract(s)
Objetivo: Otimizar o processo de formação in vitro de hidroxiapatite na presença de colagénio, a partir dos resultados anteriormente obtidos (Araújo, 2019).
Materiais e Métodos: Foram considerados os grupos de estudo de Araújo, 2019, e utilizados os materiais e métodos por este estudados e descritos. As amostras foram recobertas com ouro-paládio, observadas em SEM e sujeitas a análise EDS.
Resultados: Os valores de Ca/P encontrados foram superiores ao valor da hidroxiapatite pura, 1,67, no entanto as imagens de SEM foram consistentes com observações de hidroxiapatite realizadas por outros autores. Com base nestes resultados surgiu a hipótese de o mineral obtido não ser hidroxiapatite pura, mas um conjunto de formas de apatite. Para testar esta teoria, foi construído um modelo que procura estimar o balanço das percentagens dos compostos moleculares identificados, respeitando a composição elementar das amostras.
Conclusão: Este trabalho mostrou que é possível que o mineral formado in vitro não seja apenas hidroxiapatite. No entanto, tratando-se de resultados preliminares, para que esta hipótese seja cientificamente provada, é necessário analisar o mineral obtido por técnicas mais sensíveis.
Objectives: To optimize the in vitro process of hydroxyapatite formation in the presence of collagen, based on the results previously obtained (Araújo, 2019). Materials and methods: The study groups of Araújo, 2019, were considered, as well as, the materials and methods studied and described by him. The samples were covered with gold palladium, observed in SEM and subjected to EDS analysis. Results: The Ca/P values found were higher than the value of pure hydroxyapatite, 1.67, however the SEM images were consistent with observations of hydroxyapatite performed by other authors. Based on these results the hypothesis that the mineral obtained was not pure hydroxyapatite, but a set of forms of apatite, was studied. To test this theory, a model was constructed to estimate the balance of the percentages of the molecular compounds identified, respecting the elemental composition of the samples. Conclusions: This work showed that it is possible that the mineral formed in vitro is not only hydroxyapatite. However, since these are preliminary results, for this hypothesis to be scientifically proven, it is necessary to analyse the mineral obtained by more sensitive techniques.
Objectives: To optimize the in vitro process of hydroxyapatite formation in the presence of collagen, based on the results previously obtained (Araújo, 2019). Materials and methods: The study groups of Araújo, 2019, were considered, as well as, the materials and methods studied and described by him. The samples were covered with gold palladium, observed in SEM and subjected to EDS analysis. Results: The Ca/P values found were higher than the value of pure hydroxyapatite, 1.67, however the SEM images were consistent with observations of hydroxyapatite performed by other authors. Based on these results the hypothesis that the mineral obtained was not pure hydroxyapatite, but a set of forms of apatite, was studied. To test this theory, a model was constructed to estimate the balance of the percentages of the molecular compounds identified, respecting the elemental composition of the samples. Conclusions: This work showed that it is possible that the mineral formed in vitro is not only hydroxyapatite. However, since these are preliminary results, for this hypothesis to be scientifically proven, it is necessary to analyse the mineral obtained by more sensitive techniques.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Colagénio Hidroxiapatite Mineralização