A carregar...
Publicação
Resistências aos Antihelmínticos em Parasitas Gastrointestinais de Ovinos em Allier (região Auvergne, França)
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 11.28 MB | Adobe PDF |
Autores
Orientador(es)
Resumo(s)
O parasitismo gastrointestinal é uma causa importante de doença e quebras de produção de pequenos
ruminantes, causando perdas económicas significativas através da redução do bem-estar animal,
saúde e desempenho produtivo. O uso indiscriminado de anti-helmínticos conduziu à resistência
generalizada aos anti-helmínticos (RA), um problema crescente na produção pecuária mundial. Apesar
das suspeitas de redução da eficácia contra nemátodes gastrointestinais (NGI), os casos de RA
permanecem subestimados devido à falta de consciência dos produtores e veterinários, assim como
devido aos poucos estudos científicos nesta área. Neste sentido, o presente estudo tem como objetivo
caracterizar as espécies de parasitas presentes na região Allier, França, e monitorizar a eficácia dos
tratamentos anti-helmínticos contra NGI, sensibilizando simultaneamente produtores e veterinários
sobre a RA para promover o uso racional de anti-helmínticos. O estudo foi conduzido em três
explorações ovinas (A1, A2, A3) em Allier, região Auvergne, França. Um total de 129 amostras fecais
foi recolhido em ovinos adultos naturalmente infetados antes do tratamento (D0), seguido de 99
amostras recolhidas aproximadamente 14 dias após o tratamento (D14). A contagem de ovos por
grama de fezes (OPG) foi realizada usando a técnica de McMaster. Na exploração A1 foi administrada
moxidectina (0,2 mg/kg), na exploração A2 ivermectina (0,2 mg/kg), e na A3 os animais foram tratados
com uma combinação de mebendazol e closantel (15 mg/kg e 10 mg/kg, respetivamente). O teste de
redução da contagem de ovos fecais (TRCOF) foi analisado usando a plataforma ShinyEggCounts
seguindo as diretrizes da World Association for the Advancement of Veterinary Parasitology (WAAVP).
A análise parasitológica revelou que os ovinos estavam predominantemente infetados com parasitas
da família Trichostrongylidae (84%), seguidos por Eimeria spp. (47%), Dicrocoelium spp. (35%), e
Paramphistomum spp. (33%). Os resultados do TRCOF demonstraram resistência confirmada nas
explorações A1 (moxidectina: 80% de redução, IC 95%: 0,65-0,90) e A3 (mebendazol + closantel: 53%
de redução), enquanto a exploração A2 mostrou resistência potencial à ivermectina (97% de redução,
IC 95%: 0,87-1,00). A análise da população total revelou uma redução da contagem de ovos fecais de
88% (IC 95%: 0,78-0,95), indicando resistência generalizada aos anti-helmínticos na região estudada.
A implementação de análises coprológicas de rotina e estratégias de desparasitação seletiva é
essencial para o uso racional de anti-helmínticos e prevenção de resistências. Os resultados enfatizam
a necessidade urgente de uma mudança de paradigma nas estratégias de controlo parasitário para
abordar a crescente ameaça da resistência aos anti-helmínticos na produção pecuária.
Gastrointestinal parasitism represents a major concern in small ruminant production, causing significant economic losses through reduced animal welfare, health, and productive performance. The indiscriminate use of anthelmintics has led to widespread anthelmintic resistance (AR), a growing problem in livestock production worldwide. Despite suspected efficacy reductions against gastrointestinal nematodes (GIN), AR cases remain underestimated due to lack of awareness among producers and veterinarians, and insufficient research publication. This study aimed to characterize parasite species present in Allier, France, and monitor the efficacy of anthelmintic treatments against GIN, while raising awareness about AR among producers and veterinarians to promote rational anthelmintic use. The study was conducted on three sheep farms (A1, A2, A3) in Allier, Auvergne region, France. A total of 129 fecal samples were collected from naturally infected adult sheep before treatment (D0), followed by 99 samples collected approximately 14 days post-treatment (D14). Egg counts per gram of feces (EPG) were performed using the McMaster technique. In farm A1 was administered moxidectin (0.2 mg/kg), inn farm A2 ivermectin (0.2 mg/kg), and animals from farm A3 were treated with a combination of mebendazole and closantel (15 mg/kg and 10 mg/kg, respectively). Fecal egg count reduction test (FECRT) was analyzed using the ShinyEggCounts platform following World Association for the Advancement of Veterinary Parasitology (WAAVP) guidelines. Parasitological analysis revealed that sheep were predominantly infected with parasites of Trichostrongylidae family (84%), followed by Eimeria spp. (47%), Dicrocoelium spp. (35%), and Paramphistomum spp. (33%). FECRT results demonstrated confirmed resistance in farms A1 (moxidectin: 80% reduction, 95% CI: 0.65-0.90) and A3 (mebendazole + closantel: 53% reduction), while farm A2 showed potential resistance to ivermectin (97% reduction, 95% CI: 0.87-1.00). Overall population analysis revealed an 88% fecal egg count reduction (95% CI: 0.78-0.95), indicating widespread anthelmintic resistance in the studied region. Implementation of routine coprological analyses and selective deworming strategies is essential for rational anthelmintic use and resistance prevention. The findings emphasize the urgent need for a paradigm shift in parasite control strategies to address the growing threat of anthelmintic resistance in livestock production.
Gastrointestinal parasitism represents a major concern in small ruminant production, causing significant economic losses through reduced animal welfare, health, and productive performance. The indiscriminate use of anthelmintics has led to widespread anthelmintic resistance (AR), a growing problem in livestock production worldwide. Despite suspected efficacy reductions against gastrointestinal nematodes (GIN), AR cases remain underestimated due to lack of awareness among producers and veterinarians, and insufficient research publication. This study aimed to characterize parasite species present in Allier, France, and monitor the efficacy of anthelmintic treatments against GIN, while raising awareness about AR among producers and veterinarians to promote rational anthelmintic use. The study was conducted on three sheep farms (A1, A2, A3) in Allier, Auvergne region, France. A total of 129 fecal samples were collected from naturally infected adult sheep before treatment (D0), followed by 99 samples collected approximately 14 days post-treatment (D14). Egg counts per gram of feces (EPG) were performed using the McMaster technique. In farm A1 was administered moxidectin (0.2 mg/kg), inn farm A2 ivermectin (0.2 mg/kg), and animals from farm A3 were treated with a combination of mebendazole and closantel (15 mg/kg and 10 mg/kg, respectively). Fecal egg count reduction test (FECRT) was analyzed using the ShinyEggCounts platform following World Association for the Advancement of Veterinary Parasitology (WAAVP) guidelines. Parasitological analysis revealed that sheep were predominantly infected with parasites of Trichostrongylidae family (84%), followed by Eimeria spp. (47%), Dicrocoelium spp. (35%), and Paramphistomum spp. (33%). FECRT results demonstrated confirmed resistance in farms A1 (moxidectin: 80% reduction, 95% CI: 0.65-0.90) and A3 (mebendazole + closantel: 53% reduction), while farm A2 showed potential resistance to ivermectin (97% reduction, 95% CI: 0.87-1.00). Overall population analysis revealed an 88% fecal egg count reduction (95% CI: 0.78-0.95), indicating widespread anthelmintic resistance in the studied region. Implementation of routine coprological analyses and selective deworming strategies is essential for rational anthelmintic use and resistance prevention. The findings emphasize the urgent need for a paradigm shift in parasite control strategies to address the growing threat of anthelmintic resistance in livestock production.
Descrição
Palavras-chave
Ovinos Nemátodes gastrointestinais Resistência aos anti-helmínticos Teste de redução da contagem de ovos fecais Anthelmintic resistance Fecal egg count reduction test Gastrointestinal nematodes Sheep