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- Technical Note: “Mitochondrial and nuclear DNA approaches for reliable identification of Lucilia (Diptera, Calliphoridae) species of forensic interest from Southern Europe”Publication . GilArriortua, Maite; Saloña-Bordas, Marta I.; Cainé, Laura; Pinheiro, Fátima; M. de Pancorbo, Marian
- Genetic Data of Four X-Chromosomal STRs in a Population Sample of Santa Catarina, BrazilPublication . Cainé, Laura; Pontes, Lurdes; Abrantes, David; Lima, Gabriela; Pinheiro, FátimaA total of 184 healthy unrelated individuals (70 females and 114 males), autochthonous from Santa Catarina, Brazil.
- Genetic Data of 4 X-Chromosomal Short Tandem Repeats in a North of Portugal PopulationPublication . Cainé, Laura; Pontes, Lurdes; Abrantes, David; Lima, Gabriela; Pinheiro, FátimaOne hundred unrelated females and 100 unrelated males, autochthonous, healthy, from the North of Portugal.
- Genetic Data of Five STR Loci in a Population Sample of Santa Catarina, BrazilPublication . Pinheiro, Fátima; Lima, Gabriela; Abrantes, David; Pontes, Lurdes; Cainé, LauraIn routine forensic casework, it is important to establish a population database for further reliable statistical analysis.
- Allele Frequencies of 15 Loci Using AmpFℓSTR Identifiler Kit in a Northern Portuguese PopulationPublication . Abrantes, David; Lima, Gabriela; Cainé, Laura; Pontes, Lurdes; Pinheiro, Fátima250 unrelated and healthy individuals from Portugal.
- Analysis of 10 X-linked tetranucleotide markers in mixed and isolated populationsPublication . Zarrabeitia, María T.; Pinheiro, Fátima; de Pancorbo, Marian M.; Cainé, Laura; Cardoso, Sergio; Gusmão, Leonor; Riancho, José A.We have applied a recently described X-STRs decaplex to characterize four population groups of the Iberian Peninsula, including two well mixed populations and two relatively isolated ones from Northern Spain, in order to get a better insight about the characteristics of X-STRs in those population types between-population differences in allelic frequencies were relatively small. Nevertheless, Fst values were between 0.2 and 2.7%, figures higher than usually reported for autosomic STRs. This result suggests that when forensic cases originate from relatively isolated groups in western Europe, and a specific reference database is not available, it is probably safe to include a Fst-based correction in the calculations of matching or kinship probabilities.
- A GEP-ISFG collaborative study on the optimization of an X-STR decaplex: data on 15 Iberian and Latin American populationsPublication . Gusmão, Leonor; Sánchez-Diz, Paula; Alves, Cíntia; Gomes, Iva; Zarrabeitia, María Teresa; Abovich, Mariel; Atmetlla, Ivannia; Bobillo, Cecilia; Bravo, Luisa; Builes, Juan; Cainé, Laura; Calvo, Raquel; Carvalho, Elizeu; Carvalho, Mónica; Cicarelli, Regina; Catelli, Laura; Corach, Daniel; Espinoza, Marta; García, Óscar; Malaghini, Marcelo; Martins, Joyce; Pinheiro, Fátima; João Porto, Maria; Raimondi, Eduardo; Riancho, Jose Antonio; Rodríguez, Amelia; Rodríguez, Anayanci; Rodríguez Cardozo, Belén; Schneider, Vicente; Silva, Sandra; Tavares, Celso; Toscanini, Ulises; Vullo, Carlos; Whittle, Martin; Yurrebaso, Iñaki; Carracedo, Ángel; Amorim, AntónioIn a collaborative work carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG), a polymerase chain reaction multiplex was optimized in order to type ten X-chromosome short tandem repeats (STRs) in a single reaction, including: DXS8378, DXS9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7133, GATA172D05, GATA31E08, and DXS7423. Using this X-decaplex, each 17 of the participating laboratories typed a population sample of approximately 200 unrelated individuals (100 males and 100 females). In this work, we report the allele frequencies for the ten X-STRs in 15 samples from Argentina (Buenos Aires, Córdoba, Río Negro, Entre Ríos, and Misiones), Brazil (São Paulo, Rio de Janeiro, Paraná, and Mato Grosso do Sul), Colombia (Antioquia), Costa Rica, Portugal (Northern and Central regions), and Spain (Galicia and Cantabria). Gene diversities were calculated for the ten markers in each population and all values were above 56%. The average diversity per locus varied between 66%, for DXS7133, and 82%, for DXS6809. For this set of STRs, a high discrimination power was obtained in all populations, both in males (> or =1 in 5 x 10(5)) and females (> or =1 in 3 x 10(9)), as well as high mean exclusion chance in father/daughter duos (> or =99.953%) and in father/mother/daughter trios (> or =99.999%). Genetic distance analysis showed no significant differences between northern and central Portugal or between the two Spanish samples from Galicia and Cantabria. Inside Brazil, significant differences were found between Rio de Janeiro and the other three populations, as well as between São Paulo and Paraná. For the five Argentinean samples, significant distances were only observed when comparing Misiones with Entre Ríos and with Río Negro, the only two samples that do not differ significantly from Costa Rica. Antioquia differed from all other samples, except the one from Río Negro.
- Cytochrome b as a useful tool for the identification of blowflies of forensic interest (Diptera, Calliphoridae)Publication . GilArriortua, Maite; Salona Bordas, Marta I.; Cainé, Laura; Pinheiro, Fátima; de Pancorbo, Marian M.
- DNA typing of Diptera collected from human corpses in PortugalPublication . Cainé, Laura; Corte Real, Francisco; Saloña-Bordas, Marta I.; Martínez de Pancorbo, M.; Lima, Gabriela; Magalhães, Teresa; Pinheiro, FátimaMedico-legal entomology, one area in the broad field of entomology, is routinely used in forensic applications. Insects are often collected from a corpse during criminal information related to the body, but requires the fast and accurate identification of the species attracted to the remains. The local entomofauna in most cases is important for explaining entomological evidence. The survey of the local entomofauna has become a fundamental first step in forensic entomological studies, because different geographical distributions, seasonal and environmental factors may influence the decomposition process and the occurrence of different species on corpses. A morphological and DNA-based methods for species identification were used in this study. Thirty-two cases are reported from indoors and outdoors conditions. Specimens were collected from corpses during autopsy procedures in the National Institute of Legal Medicine, Portugal, and cases were summarized by sex, death local, month of discovery, probable cause of death, species found and number of analyzed specimens. Just eight species, mainly Calliphoridae together with one Sarcophagidae were reported from corpses. The DNA sequencing was performed to study the mitochondrial encoded subunit I of the cytochrome oxidase gene. The aim of this work was the beginning of a database of the cadaveric entomofauna in Portugal.
- Y-chromosomal STR haplotype diversity in males from Santa Catarina, BrazilPublication . Cainé, Laura; de Pancorbo, M. Martínez; Pinheiro, FátimaOne-hundred and nine unrelated and healthy males from Santa Catarina, Brazil were included in this study. Allele frequencies and gene diversities for the loci DYS456, DYS458 and DYS448 were calculated. A comparison between our population and others was performed.