Percorrer por autor "Marques, Fernanda"
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- Antitumoral and antimicrobial activities of block copolymer micelles containing gold bisdithiolate complexesPublication . Sousa, Andreia; Santos, Joana F.; Silva, Francisco; Sousa, Sílvia A.; Leitão, Jorge H.; Matos, António P.; Pinheiro, Teresa; Silva, Rafaela A. L.; Belo, Dulce; Almeida, Manuel; Marques, Fernanda; Fernandes, CéliaGold(III) bisdithiolate complexes have been reported as potential antimicrobial and antitumoral agents. The complex [Au(cdc)2]− (cdc=cyanodithioimido carbonate) displayed antimicrobial and outstanding antitumor activity against the ovarian cancer cells A2780 and A2780cisR, which are sensitive and resistant to cisplatin, respectively. However, poor water solubility may hamper its clinical use. Block copolymer micelles (BCMs) may solubilize hydrophobic drugs, improving their bioavailability and circulation time in blood. Aiming to provide water solubility, prolonged availability, and enhanced therapeutic indexes, BCMs loaded with [Au(cdc)2]− were synthesized and characterized. The BCM-[Au(cdc)2] micelles were prepared with a loading efficiency of 64.6% and a loading content of 35.3 mg [Au(cdc)2]−/gBCM. A hydrodynamic diameter of 77.31 ± 27.00 nm and a low polydispersity index of 0.18 indicated that the micelles were homogenous and good candidates for drug delivery. Cytotoxic activity studies against A2780/A2780cisR cells showed that BCM-[Au(cdc)2] maintained relevant cytotoxic activity comparable to the cytotoxicity observed for the same concentration of gold complexes. The Au uptake in A2780 cells, determined by PIXE, was ca. 17% higher for BCMs-[Au(cdc)2] compared to [Au(cdc)2]−. The BCMs-[Au(cdc)2] presented antimicrobial activity against S. aureus Newman and C. glabrata CBS138. These results evidenced the potential of BCM-[Au(cdc)2] for drug delivery and its promising anticancer and antimicrobial activities.
- Ausência do pretenso pai: qual a melhor alternativa numa investigação de paternidade?Publication . Dario, Ana Rita; Marques, Fernanda; Carvalho, Mónica; Amorim, António; Dourado, Catarina G.A maioria das perícias de investigação de parentesco biológico, realizadas pelo Serviço de Genética e Biologia Forense (SGBF), são requisitadas por ordem do Tribunal. A colheita de material biológico é realizada aos intervenientes que, habitualmente, consiste num trio constituído por pretenso pai, mãe e filho/a. No entanto, poderão existir alterações quanto ao número e tipo de intervenientes. Em situações em que, por algum motivo, o pretenso pai não está disponível, não são raras as vezes que o Tribunal questiona o SGBF, sobre quais os intervenientes, familiares do pretenso pai, poderão ser envolvidos na investigação, de modo a que os resultados estatísticos sejam mais robustos. Nestes casos, recorre-se frequentemente aos pretensos avós paternos, irmãos do pretenso pai ou filhos do pretenso pai. Assim, quanto maior o número de familiares diretos para o estudo genético, maior será a probabilidade de paternidade que pode ser obtida. O objetivo deste trabalho consiste na análise de uma perícia de investigação de parentesco biológico, com ausência de pretenso pai, mas com a presença de vários familiares deste, e como a utilização de diferentes combinações destes perfis genéticos nos cálculos estatísticos, conduzem ou não a diferenças significativas nos valores de Índice de Paternidade (IP) obtidos. As zaragatoas bucais, colhidas a cada interveniente, foram extraídas com Prep-n-Go Buffer (Applied Biosystems™) e com Chelex® 100 (Sigma-Aldrich®), amplificadas para STRs autossómicos com os kits GlobalFiler™ PCR Amplification Kit (Applied Biosystems™), PowerPlex® Fusion 6C System (Promega Corporation), e Investigator® HDplex Kit (Qiagen) e amplificadas para STRs do cromossoma Y com Yfiler™ Plus PCR Amplification Kit (Applied Biosystems™). A análise de fragmentos foi efetuada por eletroforese capilar no sequenciador automático 3500 Genetic Analyzer (Applied Biosystems™) e analisadas com o software GeneMapper® ID-X 1.4 (Applied Biosystems™). A análise estatística foi realizada utilizando o programa Familias 3. Foram obtidos resultados de IP bem distintos, consoante o tipo de relação biológica dos intervenientes, relativamente ao pretenso pai, utilizados nos cálculos estatísticos, realçando, uma vez mais, a preferência de determinados familiares em detrimento de outros.
- Dose rate effects on the selective radiosensitization of prostate cells by GRPR-targeted gold nanoparticlesPublication . Marques, Ana; Belchior, Ana; Silva, Francisco; Marques, Fernanda; Campello, Maria Paula Cabral; Pinheiro, Teresa; Santos, Pedro; Santos, Luis; Matos, António P. A.; Paulo, AntónioFor a while, gold nanoparticles (AuNPs) have been recognized as potential radiosensitizers in cancer radiation therapy, mainly due to their physical properties, making them appealing for medical applications. Nevertheless, the performance of AuNPs as radiosensitizers still raises important questions that need further investigation. Searching for selective prostate (PCa) radiosensitizing agents, we studied the radiosensitization capability of the target-specific AuNP-BBN in cancer versus non-cancerous prostate cells, including the evaluation of dose rate effects in comparison with non-targeted counterparts (AuNP-TDOTA). PCa cells were found to exhibit increased AuNP uptake when compared to non-tumoral ones, leading to a significant loss of cellular proliferation ability and complex DNA damage, evidenced by the occurrence of multiple micronucleus per binucleated cell, in the case of PC3 cells irradiated with 2 Gy of γ-rays, after incubation with AuNP-BBN. Remarkably, the treatment of the PC3 cells with AuNP-BBN led to a much stronger influence of the dose rate on the cellular survival upon γ-photon irradiation, as well as on their genomic instability. Overall, AuNP-BBN emerged in this study as a very promising nanotool for the efficient and selective radiosensitization of human prostate cancer PC3 cells, therefore deserving further preclinical evaluation in adequate animal models for prostate cancer radiotherapy.
- Gold(III) bisdithiolate complexes: molecular conductors that also exhibit anticancer and antimicrobial activitiesPublication . Marques, Fernanda; Sousa, Sílvia A.; Leitão, Jorge H.; Morais, Tânia S.; Le Gal, Yann; Lorcy, Dominique
- Modification of ZnO nanoparticles with silanes enables their application as anticancer agentsPublication . Marques, Fernanda; Tǎbǎcarub, Aurel; Bușilǎc, Mariana; Pinheiro, Teresa; Matos, António P. A.
- The Mössbauer effect using 57Fe-ferrabisdicarbollide ([o-57FESAN]−) : a glance into the potential of a low-dose approach for glioblastoma radiotherapyPublication . Buades, Ana B.; Pereira, Laura C. J.; Vieira, Bruno J. C.; Cerdeira, Ana C.; Waerenborgh, João C.; Pinheiro, Teresa; Matos, António P. A.; Pinto, Catarina G.; Guerreiro, Joana F.; Mendes, Filipa; Valic, Srecko; Teixidor, Francesc; Viñas, Clara; Marques, FernandaAlthough a variety of cancers are initially susceptible to chemotherapy, they eventually develop multi-drug resistance. To overcome this situation, more effective and selective treatments are necessary using anti-tumour agents that act in two or more ways and offer greater therapeutic benefits over single-mechanism entities. In this study, we report on treating cancer with Na[3,3′-57Fe(1,2-C2B9H11)2], which offers the possibility of dual action (radiation–drug combinations) to improve the clinical benefits and reduce healthy tissue toxicity. An approach to evaluating the potential of [o-57FESAN]− to treat glioblastoma using the Mössbauer effect is presented. As the therapeutic outcomes rely on the amount and distribution of [o-57FESAN]− inside the cells, several studies, using magnetization, Mössbauer spectroscopy and nuclear microscopy techniques, were performed to ascertain the uptake of [o-57FESAN]− in U87 glioblastoma cells. [o-57FESAN]− was found to be within the cells; 29% of its uptake was in the nuclear fraction, which is a particularly desirable target, because the nucleus is the cell's control centre where DNA and the transcription machinery reside. Irradiation studies with 2D and 3D cellular models of U87 cells showed that the growth inhibition effect observed was more pronounced when [o-57FESAN]− was used in combination with the Mössbauer effect in low total dose regimens, suggesting that this procedure either alone or as adjuvant may be useful for glioblastoma treatment.
- Oxigenoterapia de alto fluxo por traqueostomia no desmame ventilatório : scoping reviewPublication . Neiva, Sandrine; Maia, Diana; Pacheco, Artur; Marques, Fernanda; Ribeiro, Olga; Oliveira, João Pedrontrodução: A ventilação mecânica invasiva prolongada acarreta custos e tem complicaçõesassociadas. Um desmame ventilatório precoce com sucesso, assume cada vez mais importância,pretendendo-se neste estudo descrever as evidências acerca da utilização da Oxigenoterapia de AltoFluxo por Traqueostomia neste processo.Metodologia: Scoping Review segundo a metodologia do Joanna Briggs Institute®, com pesquisaefetuada nas bases de dados: CINHAL, PubMed e LILACS.Resultados: Incluíram-se quatro estudos: um estudo de caso e três estudos controlados randomizados.Relativamente aos protocolos utilizados na implementação da oxigenoterapia de alto fluxo portraqueostomia, eles variaram entre diferentes fluxos, tempo de utilização e combinação com outrosmodos ventilatórios. Os principais parâmetros monitorizados foram: frequência respiratória, FiO2,SpO2, PaO2, PaCO2 e a relação PaO2/FiO2.Discussão: A utilização de oxigenoterapia de alto fluxo por traqueostomia apresenta vantagens face àutilização de outros modos ventilatórios durante o processo de desmame ventilatório. Esta estratégiarepercute-se na melhoria dos parâmetros monitorizados e na diminuição do tempo de desmame.Conclusão: As principais contribuições da oxigenoterapia de alto fluxo por traqueostomia no desmameventilatório são: melhoria da oxigenação, diminuição do esforço respiratório, aumento do volumecorrente, diminuição do tempo de desmame e melhoria da eficácia da limpeza das vias aéreas.
- Teaching and learning clinical reasoning in nursing education: A student training coursePublication . Leal, Paula; Poeira, Ana; Mendes, Diana Arvelos; Batalha, Nara; Franco, Hugo; Nunes, Lucília; Marques, Fernanda; Paden, Ljubisa; Stefaniak, Malgorzata; Pérez-Perdomo, Ana; Bangels,Lore; Lemmens, Kathleen; Amaral, GuidaClinical reasoning is an essential component of nursing. It has emerged as a concept that integrates the core competencies of quality and safety education for nurses. In cooperation with five European partners, Instituto Politécnico de Setúbal (IPS) realized the “Clinical Reasoning in Nursing and Midwifery Education and Practice” project as part of the Erasmus+ project. As a partner, our team designed a multiplier event—the student training course. The aim of this report is to describe the construction and development of this clinical reasoning training course for nursing students. We outline the pedagogical approach of an undergraduate training course on clinical reasoning in 2023, which we separated into four stages: (i) welcoming, (ii) knowledge exploration, (iii) pedagogical learning, and (iv) sharing experience. This paper presents the learning outcomes of the collaborative reflection on and integration of the clinical reasoning concept among nursing students. This educational experience fostered reflection and discussion within the teaching team of the nursing department regarding the concept, models, and teaching/learning methods for clinical reasoning, with the explicit inclusion of clinical reasoning content in the nursing curriculum. We highlight the importance of implementing long-term pedagogical strategies in nursing education.