Percorrer por autor "Fantatto, Rafaela"
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- First insights into macromolecular components analyses of an insect meal using hyperspectral imagingPublication . Silva, Flávia Matias Oliveira da; Fidalgo, Liliana G.; Inácio, Rita S.; Fantatto, Rafaela; Carvalho, Maria J.; Murta, Daniel; Pereira, Nuno S. A.The non-invasive nutritional analysis of feed through images captured by hyperspectral cameras represents an innovative and promising approach in the field of biotechnology. With this technology, it is possible to capture images at multiple wavelengths and identify unique spectral patterns associated with different molecular components, such as total fat and moisture. The technique is particularly valuable in biological environments, enabling a more detailed and comprehensive analysis of the presence and distribution of nutrients. The objective of this work was to perform a nutritional pre-characterization of Hermetia illucens (Black Soldier Fly-BSF) larvae meal, using hyperspectral images in the range of 400–1000 nm, with a spectral resolution of 7 nm and a spatial sampling of 512 pixels, and correlate them to traditional chemical analysis methods. The results were also compared to wheat flour samples. The chemical results of BSF meal indicated 7.2% ± 0.05% (w/w) and 28.15% ± 0.15% (w/w) in moisture and total fat content, respectively. When compared to wheat flour, BSF meal contains less total fat and this difference was clearly identified in the spectra derived from the hyperspectral images, which underscores the efficiency and utility of multispectral cameras to conduct real-time and non-destructive analyses. Furthermore, this analysis can be extended to other components (e.g., protein content), increasing the potential of the technique for feed characterization.
- From waste to resource : performance of Black Soldier fly larvae reared on restaurant food waste at an industrial scalePublication . Oliveira, Joana; Ligeiro, Carolina; Fantatto, Rafaela; Souza, Clarice Silva e; Machado, Maria Ana; Guilgur, Leonardo Gaston; Trindade, Alexandre; Assunção, Ricardo; Murta, DanielFood waste poses significant environmental, economic and public health challenges. The black soldier fly (Hermetia illucens) larvae (BSFL) represent a promising solution for organic waste valorisation, converting substrates into protein-rich biomass for animal feed and organic fertiliser. However, the use of food waste as an insect substrate remains prohibited in the European Union due to regulatory and safety concerns. This study evaluated the suitability of heterogeneous food waste for BSFL rearing under industrial conditions by comparing larval performance on a standard Gainesville diet (control) and a blend derived from local restaurant waste (test). The 14-day bioconversion assay assessed bioconversion rate (BCR), feed conversion ratio (FCR), survival rate, average growth rate, and nutritional composition. Compared with the control, the test group showed significantly improved (p < 0.001) BCR (18.34% vs. 11.02%), FCR (5.48 vs. 9.09 kg/kg), survival (69.29% vs. 51.30%), and growth (8.38 vs. 6.59 mg/day). Larvae reared on food waste also exhibited significantly higher protein (19.70% vs. 16.80%), fat (13.70% vs. 7.20%), ash (6.97% vs. 3.51%), carbohydrates (7.00% vs. 3.60%), and fibre (5.20% vs. 2.90%). Overall, heterogeneous food waste is a suitable substrate for BSFL, supporting agrifood sustainability; however, future research should focus on standardisation of these substrates.
- Olive pomace and melon bio-byproducts from the agribusiness : a promising combination for the sustainable production of animal protein from BSF larvaePublication . Ligeiro, Carolina; Souza, Clarice Silva e; Fantatto, Rafaela; Murta, DanielThe black soldier fly (Hermetia illucens) offers a sustainable solution for converting agro-industrial bio-byproducts into high-quality animal protein. This study evaluated two abundant residues from the Santarém region of Portugal, melons and olive pomace, as substrates for larval rearing. Two experimental diets were formulated using these byproducts and compared to a standard Gainesville-based control diet, with all diets adjusted for moisture and protein content. Larval performance was assessed through metrics including individual weight, biomass, feed conversion ratio (FCR) and bioconversion rate. Diets 1 (melon-based) and 2 (melon + 55.2% olive pomace) yielded larval biomass of 1.82 ± 0.17 kg and 1.80 ± 0.11 kg per box, respectively, compared to the control (1.60 ± 0.03 kg). The FCRs were lower in the experimental diets (7.47 ± 0.75 and 7.53 ± 0.49) than in the control (8.42 ± 0.16), and the bioconversion rates were higher (13.48% and 13.31% vs. 11.88%). These results suggest that combining melon and olive pomace improves larval development and conversion efficiency, compared to the literature. This study demonstrates the potential of using local bio-byproducts to enhance insect-based protein production while reducing waste and supporting circular economy practices.