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Níveis de nitrito na saliva e a sua correlação com a severidade da doença periodontal e os valores de tensão arterial
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Abstract(s)
Objetivo: Estudar a correlação entre a concentração de nitritos (NO2-) na saliva, a severidade da doença periodontal (DP) e os valores de pressão arterial por comparação de dois grupos de estudo constituídos por pacientes com e sem DP.
Materiais e Métodos: Foram selecionados 45 pacientes da Clínica Dentária Egas Moniz, que cumpriam cumulativamente os critérios de inclusão e exclusão propostos, aos quais foram aplicados consentimentos informados e questionários, foi medida a pressão arterial (PA) e recolhidas amostras de 2mL de saliva não estimulada. As amostras foram posteriormente analisadas com recurso ao biossensor NO2Probe, obtendo-se assim a concentração de nitrito para cada indivíduo. Numa amostra mais restrita, foi ainda avaliada a resposta de brancos não enzimáticos. Após análise dos dados obtidos, foram ainda aplicados dois critérios de exclusão analíticos adicionais, o que reduziu a amostra a 34 participantes. Os resultados finais foram analisados estatisticamente. Adicionalmente, testou-se um protocolo de quantificação de nitratos na saliva com utilização do NO2Probe.
Resultados: Comparando os valores de concentração de nitrito em saliva de indivíduos diagnosticados com e sem DP, não foi possível estabelecer diferenças estatisticamente significativas. Embora estes resultados sejam ainda preliminares, isto pode ser indicativo de ausência de correlação entre os níveis de nitrito na saliva e a DP. Na análise comparativa dos valores de concentração de nitrito com os valores de PA, não foi possível estabelecer qualquer tendência entre os dois. No entanto, ao associarmos os valores de tensão com a presença ou ausência de DP, os pacientes comprometidos apresentaram níveis de tensão arterial mais elevados, apesar de os valores obtidos não serem estatisticamente diferentes.
Conclusão: No futuro deve-se ponderar correlacionar os valores de concentrações de nitrito com outros fatores, tais como a dieta, os valores de nitrito circulante na corrente sanguínea, a microflora e os valores de nitrato.
Objective: To study the correlation between the concentration of nitrite in the saliva, the severity of periodontal disease and the blood pressure values by comparing two study groups made up of patients with and without periodontal disease. Materials and Methods: Forty-five patients from the Egas Moniz Dental Clinic who met the proposed inclusion and exclusion criteria were selected and given informed consent and questionnaires, their blood pressure was measured and 2mL samples of unstimulated saliva were taken. The samples were then analysed using the NO2Probe biosensor, thus obtaining the nitrite concentration for each individual. In a more restricted sample, the response of non-enzymatic blanks was also evaluated. After analysing the data obtained, two additional analytical exclusion criteria were applied, which reduced the sample to 34 participants. The final results were analysed statistically. In addition, a protocol for quantifying nitrates in saliva was tested using the NO2Probe. Results: Comparing the nitrite concentration values in the saliva of individuals diagnosed with and without periodontal disease, it was not possible to establish statistically significant differences. Although these results are still preliminary, this may indicate that there is no correlation between nitrite levels in saliva and periodontal disease. When comparing nitrite concentration values with blood pressure values, it was not possible to establish any trend between the two. However, when we associated blood pressure values with the presence or absence of periodontal disease, the compromised patients had higher blood pressure levels, although the values obtained were not statistically different. Conclusion: In the future, consideration should be given to correlating nitrite concentration values with other factors, such as diet, circulating nitrite values in the bloodstream, microflora and nitrate values.
Objective: To study the correlation between the concentration of nitrite in the saliva, the severity of periodontal disease and the blood pressure values by comparing two study groups made up of patients with and without periodontal disease. Materials and Methods: Forty-five patients from the Egas Moniz Dental Clinic who met the proposed inclusion and exclusion criteria were selected and given informed consent and questionnaires, their blood pressure was measured and 2mL samples of unstimulated saliva were taken. The samples were then analysed using the NO2Probe biosensor, thus obtaining the nitrite concentration for each individual. In a more restricted sample, the response of non-enzymatic blanks was also evaluated. After analysing the data obtained, two additional analytical exclusion criteria were applied, which reduced the sample to 34 participants. The final results were analysed statistically. In addition, a protocol for quantifying nitrates in saliva was tested using the NO2Probe. Results: Comparing the nitrite concentration values in the saliva of individuals diagnosed with and without periodontal disease, it was not possible to establish statistically significant differences. Although these results are still preliminary, this may indicate that there is no correlation between nitrite levels in saliva and periodontal disease. When comparing nitrite concentration values with blood pressure values, it was not possible to establish any trend between the two. However, when we associated blood pressure values with the presence or absence of periodontal disease, the compromised patients had higher blood pressure levels, although the values obtained were not statistically different. Conclusion: In the future, consideration should be given to correlating nitrite concentration values with other factors, such as diet, circulating nitrite values in the bloodstream, microflora and nitrate values.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Doença periodontal Nitrito Hipertensão arterial Saliva