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Desproteinização da dentina e o seu efeito nas propriedades à superfície : resistência adesiva à microtração
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Abstract(s)
Objetivos: Avaliar e comparar a resistência adesiva à microtração (μTBS) de duas resinas auto-adesivas flowable, no imediato, variando o tipo de tratamento de desproteinização da superfície dentinária.
Materiais e métodos: Foram utilizados 30 molares humanos definitivos, hígidos, cuja dentina coronária média foi exposta com o auxílio de um micrótomo. Estes dentes foram divididos aleatoriamente em 3 grupos (n=10) consoante o agente desproteinizante aplicado na dentina e, de seguida, sub-divididos por duas resinas auto-adesivas (n=5), Constic (DMG, Alemanha) e VertiseTM Flow (Kerr, EUA), originando 6 grupos: Cons_H2O; VerF_ H2O; Cons_NaOCl; VerF_NaOCl; Cons_HOCl; VerF_HOCl. Os tratamentos da superfície dentinária consistiram na aplicação de água (H2O), hipoclorito de sódio (NaOCl) ou ácido hipocloroso + cloreto de estrôncio (HOCl + SrCl2). Sobre a dentina tratada foi realizado o procedimento restaurador, seguindo as instruções do fabricante. Após 24 h em água destilada a 37oC, os dentes foram seccionados em palitos (1,2 ± 0,2 mm) e posteriormente foram testados numa máquina de testes universal (1 kN; 0,5 mm/min). As superfícies de fratura foram observadas num estereomiscroscópio e o tipo de falha foi registado. Os dados foram analisados estatisticamente através de testes ANOVA two-way e Tukey’s HSD post-hoc (α = 0,05).
Resultados: A análise da ANOVA two-way revelou que tanto o tipo de resina (F=8,1; p=0,0088) quanto o tratamento da superfície (F=5,7; p=0,0084) influenciaram significativamente a resistência adesiva à microtração (μTBS), sendo que o tratamento de superfície teve um impacto ligeiramente maior. O teste post-hoc de Tukey revelou apenas uma diferença estatisticamente significativa entre o grupo Cons_H2O e VerF_HOCl (p=0,000787), destacando a eficácia da combinação Vertise Flow e HOCl + SrCl2 na adesão dentinária. A resina Vertise Flow apresentou, de maneira geral, valores médios de resistência adesiva superiores aos da resina Constic.
Conclusão: A desproteinização com estratégias alternativas ao hipoclorito de sódio, como o HOCl + SrCl2 pode ser uma abordagem promissora na melhoria da resistência adesiva de resinas auto-adesivas. Contudo, será preciso testar diferentes formulações de resinas auto-adesivas, e mais estratégias análogas.
Objectives: To evaluate and compare the microtensile bond strength (μTBS) of two flowable self-adhesive resin composites, at 24 h, upon variation of the type of dentin surface deproteinization treatment. Materials and Methods: Thirty sound, permanent human molars were used, with the middle coronal dentin exposed using a microtome. These teeth were randomly divided into 3 groups (n=10) according to the deproteinizing agent applied to the dentin, and then further subdivided into two self-adhesive resin composites (n=5), Constic (DMG, Germany) and VertiseTM Flow (Kerr, USA), resulting in 6 groups: Cons_H2O; VerF_H2O; Cons_NaOCl; VerF_NaOCl; Cons_HOCl; VerF_HOCl. Dentin surface treatments consist of applying water (H2O), sodium hypochlorite (NaOCl) or hypochlorous acid + strontium chloride (HOCl + SrCl2). The restorative procedure was carried out on the treated dentin, following the manufacturer's instructions. After 24 hours in distilled water at 37°C, the teeth were sectioned into beams (1.2 ± 0.2 mm) and subsequently tested in a universal testing machine (1 kN; 0.5 mm/min). Fracture surfaces were observed under a stereomicroscope and the type of failure was noted. Data were statistically analysed using two-way ANOVA and Tukey’s HSD post-hoc tests (α = 0.05). Results: Two-way ANOVA analysis revealed that both the type of resin (F=8.1; p=0.0088) and the surface treatment (F = 5.7; p=0.0084) significantly influenced the adhesive strength to microtensile (μTBS), with surface treatment having a slightly greater impact. Tukey's post-hoc test revealed only one statistically significant difference, between Cons_H2O and VerF_HOCl (p=0.000787), highlighting the effectiveness of the Vertise Flow and HOCl + SrCl2 combination in dentin adhesion. Vertise Flow resin generally presented higher average adhesive strength values than Constic resin. Conclusions: Deproteinization with alternative strategies to sodium hypochlorite, such as HOCl + SrCl2, may be a promising approach to improve the adhesive strength of self-adhesive resins. However, it will be necessary to test different formulations of self-adhesive resins, and more similar strategies.
Objectives: To evaluate and compare the microtensile bond strength (μTBS) of two flowable self-adhesive resin composites, at 24 h, upon variation of the type of dentin surface deproteinization treatment. Materials and Methods: Thirty sound, permanent human molars were used, with the middle coronal dentin exposed using a microtome. These teeth were randomly divided into 3 groups (n=10) according to the deproteinizing agent applied to the dentin, and then further subdivided into two self-adhesive resin composites (n=5), Constic (DMG, Germany) and VertiseTM Flow (Kerr, USA), resulting in 6 groups: Cons_H2O; VerF_H2O; Cons_NaOCl; VerF_NaOCl; Cons_HOCl; VerF_HOCl. Dentin surface treatments consist of applying water (H2O), sodium hypochlorite (NaOCl) or hypochlorous acid + strontium chloride (HOCl + SrCl2). The restorative procedure was carried out on the treated dentin, following the manufacturer's instructions. After 24 hours in distilled water at 37°C, the teeth were sectioned into beams (1.2 ± 0.2 mm) and subsequently tested in a universal testing machine (1 kN; 0.5 mm/min). Fracture surfaces were observed under a stereomicroscope and the type of failure was noted. Data were statistically analysed using two-way ANOVA and Tukey’s HSD post-hoc tests (α = 0.05). Results: Two-way ANOVA analysis revealed that both the type of resin (F=8.1; p=0.0088) and the surface treatment (F = 5.7; p=0.0084) significantly influenced the adhesive strength to microtensile (μTBS), with surface treatment having a slightly greater impact. Tukey's post-hoc test revealed only one statistically significant difference, between Cons_H2O and VerF_HOCl (p=0.000787), highlighting the effectiveness of the Vertise Flow and HOCl + SrCl2 combination in dentin adhesion. Vertise Flow resin generally presented higher average adhesive strength values than Constic resin. Conclusions: Deproteinization with alternative strategies to sodium hypochlorite, such as HOCl + SrCl2, may be a promising approach to improve the adhesive strength of self-adhesive resins. However, it will be necessary to test different formulations of self-adhesive resins, and more similar strategies.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Adesão Camada híbrida Dentina Desproteinização