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Estudo in vitro do efeito do ozono na resistência adesiva à dentina por microtração
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Abstract(s)
Objetivos: Avaliação da resistência adesiva na dentina, por microtração (μTBS), após aplicação de diversas formas de ozono.
Materiais e Métodos: 20 molares humanos hígidos foram distribuídos aleatoriamente por 4 grupos (n=5): (1) CTR (controlo); (2) O3_GAS (ozono gasoso); (3) O3_AG (água ozonizada); (4) O3_AG+GAS (água ozonizada + gás). Inicialmente, foram realizadas cavidades classe I em todas as amostras, a fim de expor a dentina para a aplicação do ozono. Posteriormente, as amostras foram expostas a diversas formas de ozono, conforme
o grupo e restauradas recorrendo ao sistema adesivo OptibondTM FL (Kerr Corporation, Orange, CA, USA) e resina composta Filtek Z250 (3M ESPE, Seefeld, Germany) com incrementos de 2 mm, perfazendo uma altura de 3 mm acima da superfície oclusal. Após o armazenamento em água destilada durante 24h a 37ºC, as amostras foram seccionadas em palitos de 1 mm2 para serem testadas através do teste de microtração. Para análise estatística foram utilizados os testes ANOVA one-way, Tukey HSD e Games-Howell (nível de significância de 5%, SPSS v.28.0).
Resultados: Após análise estatística verificaram-se diferenças estatisticamente significativas entre o grupo CTR e os grupos de ozono (O3_GAS, O3_AG, O3_AG+GAS) (p<0,001). Contudo, entre os diferentes grupos de ozono não foram verificadas diferenças significativas (p>0,05).
Conclusão: Independentemente da forma de ozono aplicada (gás ou água), o ozono interfere na resistência adesiva à dentina por microtração (μTBS).
Objective: Evaluation of dentin bonding strength by microtensile bond strength (μTBS) after application of different forms of ozone. Materials & Methods: 20 healthy human molars were randomly assigned to 4 groups (n=5): (1) CTR (control); (2) O3_GAS (ozone gas); (3) O3_AG (ozonated water); (4) O3_AG+GAS (ozonated water + ozone gas). Initially, class I cavities were made in all the samples to expose the dentin for the application of ozone. Subsequently, the samples were treated with different forms of ozone, depending on the group, and restored using the OptibondTM FL adhesive system (Kerr Corporation, Orange, CA, USA) and Filtek Z250 composite resin (3M ESPE, Seefeld, Germany) in 2 mm increments, making a height of 3 mm above the occlusal surface. After storage in distilled water for 24 hours at 37ºC, the samples were sectioned into 1 mm2 sticks to be tested using the microtensile test. The ANOVA one-way, Tukey HSD and Games-Howell tests (5% significance level, SPSS v.28.0) were used for statistical analysis. Results: Statistical analysis showed statistically significant differences between the CTR group and the ozone groups (O3_GAS, O3_AG, O3_AG+GAS) (p<0.001). However, there were no significant differences between the different ozone groups (p>0.05). Conclusion: Regardless of the form of ozone applied (gas or water), ozone reduces the dentin microtensile bond strength.
Objective: Evaluation of dentin bonding strength by microtensile bond strength (μTBS) after application of different forms of ozone. Materials & Methods: 20 healthy human molars were randomly assigned to 4 groups (n=5): (1) CTR (control); (2) O3_GAS (ozone gas); (3) O3_AG (ozonated water); (4) O3_AG+GAS (ozonated water + ozone gas). Initially, class I cavities were made in all the samples to expose the dentin for the application of ozone. Subsequently, the samples were treated with different forms of ozone, depending on the group, and restored using the OptibondTM FL adhesive system (Kerr Corporation, Orange, CA, USA) and Filtek Z250 composite resin (3M ESPE, Seefeld, Germany) in 2 mm increments, making a height of 3 mm above the occlusal surface. After storage in distilled water for 24 hours at 37ºC, the samples were sectioned into 1 mm2 sticks to be tested using the microtensile test. The ANOVA one-way, Tukey HSD and Games-Howell tests (5% significance level, SPSS v.28.0) were used for statistical analysis. Results: Statistical analysis showed statistically significant differences between the CTR group and the ozone groups (O3_GAS, O3_AG, O3_AG+GAS) (p<0.001). However, there were no significant differences between the different ozone groups (p>0.05). Conclusion: Regardless of the form of ozone applied (gas or water), ozone reduces the dentin microtensile bond strength.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Ozono Resistência adesiva Dentina Adesão