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Body fluid identification and donor association of mock case samples: Results of two EDNAP collaborative exercises

2024-09-11Other Open access
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dc.contributor.authorHänggi, Nadescha
dc.contributor.authorAmorim, António
dc.contributor.authorAfonso Costa, Heloísa
dc.contributor.authorD. Andersen, Jeppe
dc.contributor.authorMorling, Niels
dc.contributor.authorKampmann, Marie-Louise
dc.contributor.authorCourts, Cornelius
dc.contributor.authorGosch, Annica
dc.contributor.authorNeis, Maximilian
dc.contributor.authorSyndercombe Court, Denise
dc.contributor.authorGiangasparo, Federica
dc.contributor.authorElida Fonneløp, Ane
dc.contributor.authorJohannessen, Helen
dc.contributor.authorHadrys, Thorsten
dc.contributor.authorParson, Walther
dc.contributor.authorNiederstätter, Harald
dc.contributor.authorSidstedt, Maja
dc.contributor.authorSijen, Titia
dc.contributor.authorvan den Berge, Margreet
dc.contributor.authorHanson, Erin
dc.contributor.authorBallantyne, Jack
dc.contributor.authorHaas, Cordula
dc.date.accessioned2025-01-17T10:44:16Z
dc.date.available2025-01-17T10:44:16Z
dc.date.issued2024-09-11
dc.descriptionPoster apresentado no 30th Congress of the International Society for Forensic Genetics, 9 a 13 setembro de 2024, Santiago de Compostela, Espanhapt_PT
dc.description.abstractThe European DNA Profiling Group (EDNAP) has previously evaluated the performance, robustness, and reproducibility of various mRNA markers for identifying body fluids using capillary electrophoresis (CE) and massively parallel sequencing (MPS) methods. MPS of mRNA targets is used for body fluid identification and provides information on who contributed which body fluid to a binary mixture, thereby adding important contextual aspects to a crime scene investigation. The analysis of coding region SNPs (cSNPs) in body fluid-specific transcripts allows the association of an individual’s DNA cSNP profile with the body fluid-specific RNA cSNP genotype. The Zurich Institute of Forensic Medicine in Switzerland organized two consecutive collaborative exercises within the EDNAP group to evaluate the performance of two targeted mRNA sequencing assays: the BSS cSNP assay (for blood, saliva, and semen) and the 6F cSNP assay (for six fluids/tissues, including BSS and additionally vaginal secretion, menstrual blood, and skin). Each cSNP RNA assay was accompanied by a genomic DNA assay to genotype the cSNPs in the person(s) of interest. Eleven laboratories participated in one or both of the EDNAP collaborative exercises. In each exercise, 16 mock case samples were provided by the organizers, and the laboratories could analyze additional, self-prepared stains. Participants could use either the Ion S5 or the MiSeq sequencing platform. Here, we present the compiled results of the two collaborative exercises. We investigated DNA and RNA yields, STR profiles, and RNA profiles for body fluid identification and body fluid - donor association. While the mock case samples provided information on the performance of the assays, the self-prepared stains were a blind test for the organizers to identify the body fluids and the contributors.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/53881
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectBody fluid identificationpt_PT
dc.subjectmRNA profilingpt_PT
dc.subjectCoding region SNPs (cSNPs)pt_PT
dc.subjectMassively parallel sequencing (MPS)pt_PT
dc.titleBody fluid identification and donor association of mock case samples: Results of two EDNAP collaborative exercisespt_PT
dc.typeother
dspace.entity.typePublication
oaire.citation.conferencePlaceSantiago de Compostela, Espanhapt_PT
oaire.citation.title30th Congress of the International Society for Forensic Geneticspt_PT
person.familyNameAmorim
person.familyNameAfonso Costa
person.givenNameAntónio
person.givenNameHeloísa
person.identifier.orcid0000-0002-7506-4426
person.identifier.orcid0000-0002-7823-7687
person.identifier.ridA-4414-2013
person.identifier.ridJ-5725-2015
person.identifier.scopus-author-id35253426400
person.identifier.scopus-author-id24729171700
rcaap.rightsopenAccesspt_PT
rcaap.typeotherpt_PT
relation.isAuthorOfPublication71c1447c-fe17-438b-bba1-256e3abdb87a
relation.isAuthorOfPublicatione75e9d2d-2282-46d8-a2cb-37b7edbb1156
relation.isAuthorOfPublication.latestForDiscoverye75e9d2d-2282-46d8-a2cb-37b7edbb1156

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