Loading...
Publication
Mineralização da dentina profunda na presença de agentes de reticulação : um estudo ex vivo
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 15.35 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Objetivos: Avaliar o efeito dos agentes de reticulação na mineralização ex vivo de dentina profunda, por análise da variação da microdureza de Vickers a fragmentos de dentes tratados de acordo com um protocolo anteriormente desenvolvido (Kakavas, 2020).
Materiais e métodos: 50 fragmentos de dentina profunda foram divididos, aleatoriamente, por 5 grupos experimentais (n=10): Grupo Dp0 — dentina profunda sã; Grupo Dp1 — dentina profunda tratada com HCl (10 mM), 60 s; Grupo Dp2 — dentina profunda tratada com HCl e posteriormente com uma solução de cálcio (15,8 mM) em tampão de neutralização pH 7,4 contendo fosfatos (12,6 mM), 72 h a 21 °C; Grupo Dp3
— dentina profunda tratada com HCl, com solução de glutaraldeído (5%) durante 1 h a 37 °C e posteriormente com uma solução de cálcio em tampão de neutralização pH 7,4 contendo fosfatos; Grupo Dp4 — dentina profunda tratada com HCl, com solução de genipina (0,5%) durante 1 h a 37 °C e posteriormente com uma solução de cálcio em tampão de neutralização pH 7,4 contendo fosfatos. Após o tempo de tratamento avaliou-se a microdureza de Vickers dos espécimes e os resultados obtidos foram estatisticamente analisados aplicando o teste ANOVA a um fator.
Resultados: A média dos valores médios obtidos para a microdureza de Vickers para a dentina profunda de cada grupo foram: grupo Dp0 — 66,5±5,2 HV; grupo Dp1 — 61,9±6,8 HV; grupo Dp2 — 77,7±8,4 HV; grupo Dp3 — 76,5±8,8 HV; grupo Dp4 — 66,4±8,0 HV.
Conclusão: A utilização de uma solução de mineralizante, induziu um aumento na microdureza de Vickers da dentina profunda. No entanto, o glutaraldeído não apresentou qualquer efeito favorecedor da mineralização e o consequente aumento da microdureza. Por outro lado, a adição de genipina parece ter inibido o processo de
mineralização da dentina profunda, provocando uma diminuição da microdureza.
Objective: to evaluate the effect of crosslinking agents on the ex vivo mineralization of deep dentin, by measuring the Vickers microhardness, based on previously obtained results (Kakavas, 2020). Materials and methods: 50 deep dentin fragments were randomly divided into 5 experimental groups (n=10): Group Dp0 — sound deep dentin; Group Dp1 — deep dentin treated with HCl (10 mM), 60 sec; Group Dp2 — deep dentin treated with HCl and subsequently with a calcium solution (15,8 mM) in a pH 7,4 neutralization buffer containing phosphates (12,6 mM), 72 h at 21 °C; Group Dp3 — deep dentin treated with HCl, with a glutaraldehyde solution (5%), for 1 h at 37 °C and later with a calcium solution in a pH 7,4 neutralization buffer containing phosphates; Group Dp4 — deep dentin treated with HCl, with a genipin solution (0,5%), for 1 h at 37 °C and then with a calcium solution in a pH 7,4 neutralization buffer containing phosphates. After the treatment time, the Vickers microhardness of the specimens was evaluated, and the results obtained were statistically related by applying the one-way ANOVA test. Results: The mean of the mean values obtained for the Vickers microhardness for the deep dentin of each group were: Group Dp0 — 66,5±5,2 HV; Group Dp1 — 61,9±6,8 HV; Group Dp2 — 77,7±8,4 HV; Group Dp3 — 76,5±8,8 HV; Group Dp4 — 66,4±8,0 HV. Conclusion: The use of a mineralizing solution induced an increase in the Vickers microhardness of the deep dentin. However, glutaraldehyde did not have any effect that favors mineralization and the consequent increase in microhardness. On the other hand, the addition of genipin seems to have inhibited the mineralization process of deep dentin, causing a decrease in microhardness.
Objective: to evaluate the effect of crosslinking agents on the ex vivo mineralization of deep dentin, by measuring the Vickers microhardness, based on previously obtained results (Kakavas, 2020). Materials and methods: 50 deep dentin fragments were randomly divided into 5 experimental groups (n=10): Group Dp0 — sound deep dentin; Group Dp1 — deep dentin treated with HCl (10 mM), 60 sec; Group Dp2 — deep dentin treated with HCl and subsequently with a calcium solution (15,8 mM) in a pH 7,4 neutralization buffer containing phosphates (12,6 mM), 72 h at 21 °C; Group Dp3 — deep dentin treated with HCl, with a glutaraldehyde solution (5%), for 1 h at 37 °C and later with a calcium solution in a pH 7,4 neutralization buffer containing phosphates; Group Dp4 — deep dentin treated with HCl, with a genipin solution (0,5%), for 1 h at 37 °C and then with a calcium solution in a pH 7,4 neutralization buffer containing phosphates. After the treatment time, the Vickers microhardness of the specimens was evaluated, and the results obtained were statistically related by applying the one-way ANOVA test. Results: The mean of the mean values obtained for the Vickers microhardness for the deep dentin of each group were: Group Dp0 — 66,5±5,2 HV; Group Dp1 — 61,9±6,8 HV; Group Dp2 — 77,7±8,4 HV; Group Dp3 — 76,5±8,8 HV; Group Dp4 — 66,4±8,0 HV. Conclusion: The use of a mineralizing solution induced an increase in the Vickers microhardness of the deep dentin. However, glutaraldehyde did not have any effect that favors mineralization and the consequent increase in microhardness. On the other hand, the addition of genipin seems to have inhibited the mineralization process of deep dentin, causing a decrease in microhardness.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Dentina profunda Genipina Glutaraldeído Remineralização