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Estudo do efeito da hidroxiapatite modificada com zinco por análise da microdureza de superfície do esmalte
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Advisor(s)
Abstract(s)
Objetivo: Avaliar a microdureza de superfície do esmalte, pela incorporação do catião zinco na hidroxiapatite.
Materiais e métodos: 40 espécimes foram obtidos a partir de 10 molares humanos e divididos aleatoriamente em quatro grupos (A, B, C e D). Grupo A – esmalte são. Três destes grupos (B, C e D) foram expostos à desmineralização através da solução tampão de ácido láctico a pH 5 durante 6 dias, seguidos de imersão em água destilada durante 2h, seguida da aplicação de verniz de fluoreto de sódio a 5%. Posteriormente, os grupos C e D foram tratados com soluções de saliva artificial diferentes, durante 6 dias e com renovação diária: C, imersão em saliva artificial com cálcio; D, imersão em saliva artificial com zinco. Todos os grupos foram submetidos à análise de microdureza Vickers. Após a recolha dos dados, sucedeu-se a análise estatística por aplicação do teste ANOVA One-way (intervalo de confiança de 95%), seguido de comparação múltipla pelo teste de Bonferroni (software SPSS, versão 24). Resultados: O teste ANOVA One-way e de Bonferroni garantiram que existem diferenças significativas entre os valores médios dos grupos de estudo (p<0,001 e p<0,05, respetivamente). A - 518,620 ± 4,4726; B - 320,200 ± 5,7240; C - 500,120 ± 4,1986; D - 409,920 ± 4,6878
Conclusões: A modificação na HA com a incorporação de zinco levou a um aumento da microdureza de superfície sugestivo de remineralização. Contudo, estes os valores obtidos pelo zinco foram inferiores aos do cálcio.
Objectives: Evaluate the enamel surface microhardness by incorporating the zinc cation in hydroxyapatite. Materials and methods: 40 specimens were obtained from 10 human molars and randomly divided in 4 groups (A, B, C e D). Group A – sound enamel. 3 of these groups (B, C and D) were subjected to lactic acid buffer at pH 5 for 6 days, followed by immersion in distilled water for 2h and then application of 5% sodium fluoride varnish. Subsequently, groups C and D were treated with different artificial saliva solutions, for 6 days with daily renewal: C, immersion in artificial saliva with calcium; D, immersion in artificial saliva with zinc. Group A was not subjected to demineralization neither to treatment. All groups were submitted to Vickers microhardness test. After data collection, statistical analysis was performed by applying a one-way ANOVA test (95% confidence interval), followed by multiple comparison by Bonferroni test (SPSS software, version 24). Results: ANOVA test and Bonferroni test guaranteed that there are significant differences between the mean values of the study groups (p<0,001 and p<0,05, respectively). A - 518,620 ± 4,4726; B - 320,200 ± 5,7240; C - 500,120 ± 4,1986; D - 409,920 ± 4,6878 Conclusions: The modification of hydroxyapatite, with the incorporation of zinc, led to an increase of surface microhardness suggestive of remineralization. However, these values obtained by zinc were lower than calcium.
Objectives: Evaluate the enamel surface microhardness by incorporating the zinc cation in hydroxyapatite. Materials and methods: 40 specimens were obtained from 10 human molars and randomly divided in 4 groups (A, B, C e D). Group A – sound enamel. 3 of these groups (B, C and D) were subjected to lactic acid buffer at pH 5 for 6 days, followed by immersion in distilled water for 2h and then application of 5% sodium fluoride varnish. Subsequently, groups C and D were treated with different artificial saliva solutions, for 6 days with daily renewal: C, immersion in artificial saliva with calcium; D, immersion in artificial saliva with zinc. Group A was not subjected to demineralization neither to treatment. All groups were submitted to Vickers microhardness test. After data collection, statistical analysis was performed by applying a one-way ANOVA test (95% confidence interval), followed by multiple comparison by Bonferroni test (SPSS software, version 24). Results: ANOVA test and Bonferroni test guaranteed that there are significant differences between the mean values of the study groups (p<0,001 and p<0,05, respectively). A - 518,620 ± 4,4726; B - 320,200 ± 5,7240; C - 500,120 ± 4,1986; D - 409,920 ± 4,6878 Conclusions: The modification of hydroxyapatite, with the incorporation of zinc, led to an increase of surface microhardness suggestive of remineralization. However, these values obtained by zinc were lower than calcium.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Zinco Hidroxiapatite Remineralização Microdureza