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High concentrations of nordiazepam may impair the detection of benzoylecgonine by GC-MS with derivatization

2024-09Other Embargoed access
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dc.contributor.authorCosta, Suzel
dc.contributor.authorRosendo, Luana M.
dc.contributor.authorBarroso, Mário
dc.contributor.authorGallardo, Eugenia
dc.date.accessioned2025-01-30T16:27:47Z
dc.date.embargo2026-09
dc.date.issued2024-09
dc.descriptionPoster apresentado nas IV Jornadas Ibéricas de Toxicologia, 26-27 setembro de 2024, Covilhã, Portugalpt_PT
dc.description.abstractIntroduction: Forensic toxicology plays a pivotal role in legal investigations, particularly in detecting substances in biological samples. However, the simultaneous presence of multiple substances can pose analytical challenges. For instance, nordiazepam, a major metabolite of some benzodiazepines, can interfere with the detection of benzoylecgonine, a primary metabolite of cocaine, when using gas chromatography-mass spectrometry (GC-MS) with N- methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) derivatization. This interference could result in false negatives for benzoylecgonine detection. Aims: This study aims to assess whether N-methyl-N-(tert-butyldimethylsilyl)-trifluoroacetamide (MBDSTFA) derivatization can reduce interference from nordiazepam, thereby improving the detection and quantification of benzoylecgonine in biological samples. Methods: Solid phase extraction (SPE) was carried out on post-mortem blood samples using Oasis® MCX columns. Microwave derivatization with MSTFA or MBDSTFA followed after the extracts were evaporated. Results and discussion: Two derivatization reagents, MSTFA and MBDSTFA, were compared using GC-MS to evaluate their effectiveness. For this assessment, both the limit of detection (LOD) and limit of quantification (LOQ) for benzoylecgonine in this chromatographic method were 10 and 25 ng/mL, respectively. MBDSTFA derivatization significantly reduced analytical interference between nordiazepam and benzoylecgonine. In contrast, MSTFA derivatization resulted in substantial interference at high concentrations of nordiazepam, potentially leading to false negatives for cocaine detection. These findings highlight the superior performance of MBDSTFA in complex toxicological analyses. Conclusion: MBDSTFA derivatization is more effective than MSTFA for minimizing interference in the detection of benzoylecgonine in the presence of nordiazepam. This method enhances the specificity of forensic toxicological analyses and helps prevent false negatives for detecting cocaine consumption through its principal metabolite, thereby supporting more reliable legal and clinical decisions.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/54091
dc.language.isoporpt_PT
dc.peerreviewedyespt_PT
dc.relationHealth Sciences Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGC-MSpt_PT
dc.subjectPitfallspt_PT
dc.subjectbenzoylecgoninept_PT
dc.subjectNordiazepampt_PT
dc.subjectderivatizationpt_PT
dc.titleHigh concentrations of nordiazepam may impair the detection of benzoylecgonine by GC-MS with derivatizationpt_PT
dc.typeother
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.citation.conferencePlaceCovilhã, Portugalpt_PT
oaire.citation.titleIV Jornadas Ibéricas de Toxicologiapt_PT
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programático
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCosta
person.familyNameda Silva Rosendo
person.familyNameBarroso
person.familyNameGallardo
person.givenNameSuzel
person.givenNameLuana May
person.givenNameMário
person.givenNameEugenia
person.identifier.ciencia-idF611-6D58-E4C2
person.identifier.ciencia-id4E12-377B-7569
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.ciencia-idF617-5CA5-EF61
person.identifier.orcid0009-0002-1268-8052
person.identifier.orcid0000-0002-0461-8540
person.identifier.orcid0000-0001-8848-2734
person.identifier.orcid0000-0002-1802-8998
person.identifier.scopus-author-id35329243100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typeotherpt_PT
relation.isAuthorOfPublication8592c807-a8a5-4408-90d5-b32ceaf6b363
relation.isAuthorOfPublication52a4b3fa-fd99-413d-baab-ab4119bd46e9
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