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Prevalência De Estirpes Multirresistentes De Escherichia Coli, Isoladas De Vitelos Com Diarreia Neonatal Oriundos Dos Departamentos Do Cantal, Haute-Loire, Loire E Puy-De-Dôme (França)
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Abstract(s)
Escherichia coli Enterotoxigénica (ETEC), apresenta uma elevada prevalência na Diarreia Neonatal
Bovina (DNB). Esta é uma doença mul=fatorial afetando principalmente os vitelos nas primeiras
semanas de vida, levando a elevadas perdas económicas. A par=r de dados clínicos cedidos pelos
Laboratórios de TERANA, França, foi construída uma base de dados, que permi=u analisar o perfil de
virulência e de susce=bilidade a an=microbianos, entre o período de 2016 a 2022 em 2367 isolados
de E. coli provenientes de amostras fecais de vitelos suspeitos de serem portadores de DNB. A
iden=ficação dos isolados foi realizada por API 20E e Maldi-ToF MS e a pesquisa de fatores de
virulência (FV) fimbriais e não fimbriais por método de aglu=nação em lâmina. Este estudo pretendeu
ainda verificar a possível relação entre a presença de FV e perfis de mul=rresistência (MDR) nos
isolados em estudo.
No total dos isolados observou-se que 59% (1388/2367) eram portadores de FV. CS31A foi o FV mais
prevalente (61%), seguido de F5 (20%), F17 (8%), F41 e F6 (2%). Rela=vamente ao perfil de resistência
a an=microbianos (AMR), observou-se uma elevada prevalência de isolados portadores de resistência
a aminoglicosídeos e aminopenicilinas (89%); tetraciclinas (80%); quinolonas (48%) e sulfonamidas
(42%). Em outras categorias destacou-se a resistência à cefuroxima (26%); flumequina (34%),
florfenicol (27%) e à colis=na 1% (p<0.05).
Verificou-se que 85% dos isolados (2010/2367) eram portadores de perfil de MDR, dos quais 61%
(1233/2010) transportavam FV. Observou-se ainda que o perfil MDR mais prevalente incluía
resistência simultânea a aminopenicilinas, aminoglicosídeos e tetraciclinas (43,34%), associado ao FV
CS31A (47%), seguido de aminoglicosídeos, tetraciclinas e sulfonamidas em isolados não portadores
de FV (43%) (p<0.05).
Perante os resultados é possível concluir que bovinos portadores de DNB por ETEC podem cons=tuir
um reservatório não só de E. coli portadora de FV mas também de perfil MDR associado ao FV não
fimbrial CS31A, ou não associado a qualquer FV. O facto de poderem ocorrer eventos de transferência
horizontal poderá contribuir para a disseminação de FV associados a perfil MDR para E. coli não
patogénica (NPEC) endógena da microbiota intes=nal, quer dos animais, quer do Homem,
cons=tuindo um risco de saúde pública e exigindo medidas de monitorização não só de es=rpes de
ETEC mas de NPEC .
Escherichia coli Enterotoxigenic (ETEC) presents a high prevalence in Neonatal Bovine Diarrhea (NBD). This is a mul=factorial disease primarily affec=ng calves in the first weeks of life, leading to significant economic losses. Using clinical data provided by TERANA Laboratories, France, a database was constructed to analyze the virulence profile and an=microbial suscep=bility between 2016 and 2022 in 2367 E. coli isolates from fecal samples of calves suspected of having NBD. The iden=fica=on of isolates was performed by API 20E and Maldi-ToF MS, and the search for fimbrial and non-fimbrial virulence factors (VF) was conducted using the slide agglu=na=on method. This study also aimed to verify the possible rela=onship between the presence of VF and mul=drug resistance (MDR) profiles in the studied isolates. Of the total isolates, 59% (1388/2367) were carriers of VF. CS31A was the most prevalent VF (61%), followed by F5 (20%), F17 (8%), F41, and F6 (2%). Regarding the an=microbial resistance (AMR) profile, a high prevalence of isolates carrying resistance to aminoglycosides and aminopenicillins (89%); tetracyclines (80%); quinolones (48%); and sulfonamides (42%) was observed. In other categories, resistance to cefuroxime (26%); flumequine (34%); florfenicol (27%); and colis=n (1%) was notable (p<0.05). It was found that 85% of the isolates (2010/2367) carried the MDR profile, of which 61% (1233/2010) carried FV. It was also observed that the most prevalent MDR profile included simultaneous resistance to aminopenicillins, aminoglycosides and tetracyclines (43.34%), associated with FV CS31A (47%), followed by aminoglycosides, tetracyclines and sulfonamides in isolates not carrying FV (43 %) (p<0.05). The results suggest that ca|le with NBD due to ETEC can serve as a reservoir not only of E. coli carrying VF but also of MDR profiles associated with the non-fimbrial VF CS31A or not associated with any VF. The possibility of horizontal gene transfer events could contribute to the dissemina=on of VF associated with MDR profiles to non-pathogenic E. coli (NPEC) endogenous to the intes=nal microbiota, both in animals and humans, posing a public health risk and necessita=ng monitoring measures for both ETEC and NPEC strains.
Escherichia coli Enterotoxigenic (ETEC) presents a high prevalence in Neonatal Bovine Diarrhea (NBD). This is a mul=factorial disease primarily affec=ng calves in the first weeks of life, leading to significant economic losses. Using clinical data provided by TERANA Laboratories, France, a database was constructed to analyze the virulence profile and an=microbial suscep=bility between 2016 and 2022 in 2367 E. coli isolates from fecal samples of calves suspected of having NBD. The iden=fica=on of isolates was performed by API 20E and Maldi-ToF MS, and the search for fimbrial and non-fimbrial virulence factors (VF) was conducted using the slide agglu=na=on method. This study also aimed to verify the possible rela=onship between the presence of VF and mul=drug resistance (MDR) profiles in the studied isolates. Of the total isolates, 59% (1388/2367) were carriers of VF. CS31A was the most prevalent VF (61%), followed by F5 (20%), F17 (8%), F41, and F6 (2%). Regarding the an=microbial resistance (AMR) profile, a high prevalence of isolates carrying resistance to aminoglycosides and aminopenicillins (89%); tetracyclines (80%); quinolones (48%); and sulfonamides (42%) was observed. In other categories, resistance to cefuroxime (26%); flumequine (34%); florfenicol (27%); and colis=n (1%) was notable (p<0.05). It was found that 85% of the isolates (2010/2367) carried the MDR profile, of which 61% (1233/2010) carried FV. It was also observed that the most prevalent MDR profile included simultaneous resistance to aminopenicillins, aminoglycosides and tetracyclines (43.34%), associated with FV CS31A (47%), followed by aminoglycosides, tetracyclines and sulfonamides in isolates not carrying FV (43 %) (p<0.05). The results suggest that ca|le with NBD due to ETEC can serve as a reservoir not only of E. coli carrying VF but also of MDR profiles associated with the non-fimbrial VF CS31A or not associated with any VF. The possibility of horizontal gene transfer events could contribute to the dissemina=on of VF associated with MDR profiles to non-pathogenic E. coli (NPEC) endogenous to the intes=nal microbiota, both in animals and humans, posing a public health risk and necessita=ng monitoring measures for both ETEC and NPEC strains.
Description
Keywords
Antimicrobianos Diarreia Neonatal Bovina Escherichia Coli Fatores De Virulência Multirresistência Antimicrobials Neonatal Bovine Diarrhea Escherichia Coli Virulence Factors Multidrug Resistance