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A Burkholderia sacchari cell factory: production of poly-3-hydroxybutyrate, xylitol and xylonic acid from xylose-rich sugar mixtures

2017-01Journal article Restricted access
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dc.contributor.authorRaposo, Rodrigo S.
dc.contributor.authorAlmeida, M. Catarina M. D. de
dc.contributor.authorFonseca, M. Manuela da
dc.contributor.authorCesário, M. Teresa
dc.date.accessioned2019-12-09T16:05:38Z
dc.date.available2019-12-09T16:05:38Z
dc.date.issued2017-01
dc.description.abstract"Efficient production of poly-3-hydroxybutyrate (P(3HB)) based on glucose-xylose mixtures simulating different types of lignocellulosic hydrolysate (LCH) was addressed using Burkholderia sacchari, a wild strain capable of metabolizing both sugars and producing P(3HB). Carbon catabolite repression was avoided by maintaining glucose concentration below 10g/L. Xylose concentrations above 30g/L were inhibitory for growth and production. In fed-batch cultivations, pulse size and feed addition rate were controlled in order to reach high productivities and efficient sugar consumptions. High xylose uptake and P(3HB) productivity were attained with glucose-rich mixtures (glucose/xylose ratio in the feed=1.5w/w) using high feeding rates, while with xylose-richer feeds (glucose/xylose=0.8w/w), a lower feeding rate is a robust strategy to avoid xylose build-up in the medium. Xylitol production was observed with xylose concentrations in the medium above 30-40g/L. With sugar mixtures featuring even lower glucose/xylose ratios, i.e. xylose-richer feeds (glucose/xylose=0.5), xylonic acid (a second byproduct) was produced. This is the first report of the ability of Burkholderia sacchari to produce both xylitol and xylonic acid."pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationR.S. Raposo et al. /New Biotechnology, 2017 Jan 25;34:12-22. doi: 10.1016/j.nbt.2016.10.001pt_PT
dc.identifier.doi10.1016/j.nbt.2016.10.001pt_PT
dc.identifier.issn1871-6784
dc.identifier.urihttp://hdl.handle.net/10400.26/30413
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPRODUCTION OF POLY 3-HYDROXYBUTYRATE-CO- 4-HYDROXYBUTYRATE P 3HB-CO-4HB FROM LIGNOCELLULOSIC AGRO-INDUSTRIAL WASTES
dc.relationPRODUÇÃO DE POLIHIDROXIALCANOATOS UTILIZANDO SUBSTRATOS RESIDUAIS
dc.relation.publisherversionhttps://doi.org/10.1016/j.nbt.2016.10.001pt_PT
dc.subjectGlucose/xylose mixturespt_PT
dc.subjectBurkholderia sacchaript_PT
dc.subjectXylose consumptionpt_PT
dc.subjectoly-3-hydroxybutyratept_PT
dc.subjectXylitolpt_PT
dc.subjectXylonic acidpt_PT
dc.titleA Burkholderia sacchari cell factory: production of poly-3-hydroxybutyrate, xylitol and xylonic acid from xylose-rich sugar mixturespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePRODUCTION OF POLY 3-HYDROXYBUTYRATE-CO- 4-HYDROXYBUTYRATE P 3HB-CO-4HB FROM LIGNOCELLULOSIC AGRO-INDUSTRIAL WASTES
oaire.awardTitlePRODUÇÃO DE POLIHIDROXIALCANOATOS UTILIZANDO SUBSTRATOS RESIDUAIS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04565%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00100%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F68587%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F26678%2F2006/PT
oaire.citation.endPage22pt_PT
oaire.citation.startPage12pt_PT
oaire.citation.titleNew Biotechnologypt_PT
oaire.citation.volume34pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctPoítica de copyright do editorpt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication05007b22-49a3-4d43-b16d-8ad72ab05be7
relation.isProjectOfPublication23148a10-aa36-4930-a672-b67409a04c57
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