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Impacto local e sistémico da indução de periodontite em modelos animais : revisão sistemática e meta-análise
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Abstract(s)
Contexto: A periodontite é uma doença inflamatória crónica dos tecidos de suporte do dente, iniciada por uma disbiose da microbiota oral num hospedeiro suscetível. Diversos modelos de periodontite têm sido utilizados nas últimas décadas, embora permaneça incerteza sobre quais os mais adequados para a indução da periodontite. O nosso objetivo foi resumir e comparar a perda óssea alveolar de diferentes protocolos de periodontite induzida em modelos animais e as suas respetivas repercussões locais e sistémicas.
Materiais e Métodos: PubMed, Embase e Web of Science foram pesquisadas até abril de 2023. Modelos animais saudáveis com indução de periodontite que relatasse perda óssea alveolar com um grupo de controlo foram elegíveis. Os estudos foram agrupados de acordo com o método de indução. O risco de enviesamento foi avaliado através do Systematic Review Center for Laboratory animal Experimentation (SYRCLE). As
estimativas dos subgrupos foram efetuadas através de uma meta-análise de rácio de médias (ROM).
Resultados: Após seleção de 16311 artigos, foram incluídos 450. Todos os estudos utilizaram uma ou mais técnicas diferentes para induzir periodontite e compararam-nas com um grupo de controlo. Globalmente, o modelo de periodontite induzida por ligadura (n=290; effect=2.39; 95% CI: 2.21-2.58; I2=99.4%) e a combinação de ligadura e inoculação periodontal com lipopolissacáridos (LPS) bacterianos (n=10; effect=2.46;
95% CI: 2.11-2.86; I2=99. 5%) foram os métodos com maior perda de osso alveolar, seguidos pela indução de LPS (n=38; effect=2.01; 95% CI: 1.67-2.42; I2=99.6%), indução por ligadura com inoculação oral de bactérias associadas à periodontite (n=34; effect=2.11; 95% CI: 1.79-2.49; I2=99.5%), inoculação bacteriana (n=57; effect=1.60; 95% CI: 1.36-1.89; I2=99.0%) e gavagem oral (n=30; effect=1.28; 95% CI: 1.20-1.37; I2=97.5%).
Conclusões: No geral, os modelos de periodontite induzida por ligadura e a combinação de ligadura com LPS foram os modelos que melhor conseguiram mimetizar a periodontite e mostrar maior perda de osso alveolar.
Background: Periodontitis is a chronic inflammatory disease of the supporting tissues of the teeth, initiated by dysbiosis of the oral microbiota in a susceptible host. A variety of periodontitis induced models have been established over the past few decades, although there is much uncertainty about which models are suitable for the induction of periodontitis. Therefore, we aimed to summarise and compare the alveolar bone loss (ABL) of different protocols of periodontitis induction in animal models and their respective local and systemic effects. Material & Methods: PubMed, Embase and Web of Science were searched up to April 2023. Systemically healthy animal models of experimentally induced periodontitis with a control group reporting ABL were eligible for inclusion in this systematic review. Studies were subgrouped according to the disease induction method. Risk of bias (ROB) was appraised using the Systematic Review Center for Laboratory animal Experimentation (SYRCLE). Pooled estimates were made using a through ratio of means (ROM) random-effects meta-analysis. Results: After screening 16311 articles, 450 were included. All studies performed one or more different techniques to induce periodontitis and compared them with a control group. Overall, the ligature-induced periodontitis model (n=290; effect=2.39; 95% CI: 2.21-2.58; I2=99.4%) and the combination of ligature and periodontal inoculation with bacterial lipopolysaccharides (LPS) (n=10; effect=2.46; 95% CI: 2.11-2.86; I2=99.5%) were the methods with the highest ABL, followed by the LPS induction (n=38; effect=2.01; 95% CI: 1.67-2.42; I2=99.6%), the ligature-induction with oral inoculation of periodontitis-associated bacteria (n=34; effect=2.11; 95% CI: 1.79-2.49; I2=99.5%), the bacterial inoculation (n=57; effect=1.60; 95% CI: 1.36-1.89; I2=99.0%) and oral gavage (n=30; effect=1.28; 95% CI=1.20-1.37; I2=97.5%). Conclusions: Overall, ligature-induced periodontitis models and the combination of ligature with LPS were the models that best performed in mimicking periodontitis and with higher ABL.
Background: Periodontitis is a chronic inflammatory disease of the supporting tissues of the teeth, initiated by dysbiosis of the oral microbiota in a susceptible host. A variety of periodontitis induced models have been established over the past few decades, although there is much uncertainty about which models are suitable for the induction of periodontitis. Therefore, we aimed to summarise and compare the alveolar bone loss (ABL) of different protocols of periodontitis induction in animal models and their respective local and systemic effects. Material & Methods: PubMed, Embase and Web of Science were searched up to April 2023. Systemically healthy animal models of experimentally induced periodontitis with a control group reporting ABL were eligible for inclusion in this systematic review. Studies were subgrouped according to the disease induction method. Risk of bias (ROB) was appraised using the Systematic Review Center for Laboratory animal Experimentation (SYRCLE). Pooled estimates were made using a through ratio of means (ROM) random-effects meta-analysis. Results: After screening 16311 articles, 450 were included. All studies performed one or more different techniques to induce periodontitis and compared them with a control group. Overall, the ligature-induced periodontitis model (n=290; effect=2.39; 95% CI: 2.21-2.58; I2=99.4%) and the combination of ligature and periodontal inoculation with bacterial lipopolysaccharides (LPS) (n=10; effect=2.46; 95% CI: 2.11-2.86; I2=99.5%) were the methods with the highest ABL, followed by the LPS induction (n=38; effect=2.01; 95% CI: 1.67-2.42; I2=99.6%), the ligature-induction with oral inoculation of periodontitis-associated bacteria (n=34; effect=2.11; 95% CI: 1.79-2.49; I2=99.5%), the bacterial inoculation (n=57; effect=1.60; 95% CI: 1.36-1.89; I2=99.0%) and oral gavage (n=30; effect=1.28; 95% CI=1.20-1.37; I2=97.5%). Conclusions: Overall, ligature-induced periodontitis models and the combination of ligature with LPS were the models that best performed in mimicking periodontitis and with higher ABL.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Indução periodontal Modelo animal Perda óssea alveolar Revisão sistemática