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Study of gastrointestinal parasites in the iberian wolf (Canis lupus signatus)

authorProfile.affiliation
dc.contributor.advisorDuarte, Sofia Alexandra Giestas Cancela
dc.contributor.advisorMateus, Teresa Susana Letra
dc.contributor.authorPereira, Ana LuĆ­sa de Oliveira
dc.date.accessioned2016-12-27T11:14:11Z
dc.date.available2018-06-30T00:30:10Z
dc.date.issued2015-06-30
dc.description.abstractIberian wolf (Canis lupus signatus), is the largest carnivore in Iberian Peninsula and is threatened with extinction. The aim of this study was to contribute to the knowledge of helminthic and protozoa fauna, in the wolf populations from the North of Spain, with the perspective to evaluate the health and surveillance of the diseases of these packs. One hundred and seventy seven samples of faeces of Canis lupus signatus, were collected from the environment in the North of Spain, between 2013 and 2014 and submitted to coprological methods. Helminth eggs were detected on 101 (57.0 %), and simple infections were more frequent with a prevalence of 53.4 % comparing with multiple infections. In the overall samples, eggs from Capillaria spp. (41.5 %) were the most frequently detected, followed by Toxocara spp. (40.5 %), Ancylostomatidae (29.7 %), Taeniidae (26.7 %) and Trichuris spp. (25.7 %). Results are discussed and compared with earlier published studies, especially to check the present status of helminthic infections in the wolf populations in that region. Additionally a subsample was analysed using a immunofluorescent assay (IFA) to identify the presence of Giardia spp. and Cryptosporidium spp. Fifty faecal samples were analysed, of which ten (20 %) were positive. Simple infections were more frequent, with seven (14 %) samples positive for Giardia spp. and two (4 %) positive for Cryptosporidium spp. Multiple infections with both protozoan species were found in just one (2 %) sample. Faecal samples, even frozen, revealed to be a valuable tool, to detect and identify helminthic and protozoa infections from wolves in the scope of surveillance programs.pt_PT
dc.description.abstractO Lobo IbĆ©rico (Canis lupus signatus) Ć© o maior carnĆ­voro da PenĆ­nsula IbĆ©rica e encontra-se ameaƧado de extinção. O presente estudo, tem como objetivo, contribuir para o estudo e conhecimento da fauna helmĆ­ntica e protozoĆ”ria (nomeadamente Giardia spp. e Criptosporidium spp.) nas populaƧƵes de lobos do Norte de Espanha, com a perspetiva de avaliar o estado sanitĆ”rio destas populaƧƵes. Cento e setenta e sete amostras fecais de Canis lupus signatus foram recolhidas do meio ambiente entre os anos de 2013 e 2014 e foram analisadas usando mĆ©todos de coprologia. Foi detetada a presenƧa de ovos de helmintes em 101 (57,0 %), e foi mais frequente a presenƧa de infeƧƵes simples (53,4 %) comparativamente com infeƧƵes mĆŗltiplas. No total de animais infetados, incluindo infeƧƵes mĆŗltiplas, os ovos de Capillaria spp. (41,5 %) foram os mais frequentes, seguidos por Toxocara spp. (40,5 %), Ancylostomatidae (29,7 %), Taeniidae (26,7 %) e Trichuris spp. (25, 7%). Os resultados foram comparados e discutidos com estudos anteriores. Adicionalmente uma subamostra foi analisada para determinar a presenƧa de Giardia spp. e Cryptosporidium spp., usando um teste comercial de imunofluorescĆŖncia (IFA). Cinquenta amostras foram analisadas e dez (20 %) foram positivas ao teste. Foi mais frequente a presenƧa de infeƧƵes simples causadas por Giardia spp., sete (10 %) e duas (4 %) positivas para Cryptosporidium spp. e infeƧƵes mĆŗltiplas em apenas umas das amostras (2 %). A utilização de amostras fecais congeladas, revelou-se uma ferramenta Ćŗtil, para deteção e identificação de infeƧƵes por helmintes gastrointestinais e protozoĆ”rios, em lobos selvagens ā€œin- vivoā€ e em risco de extinção, no Ć¢mbito da vigilĆ¢ncia epidemiológica.pt_PT
dc.identifier.tid201108569
dc.identifier.urihttp://hdl.handle.net/10400.26/17127
dc.language.isoengpt_PT
dc.subjectCryptosporidiumpt_PT
dc.subjectGiardiapt_PT
dc.subjectHelminthspt_PT
dc.subjectParasitespt_PT
dc.subjectSpainpt_PT
dc.subjectWolfpt_PT
dc.subjectZoonosespt_PT
dc.subjectEspanhapt_PT
dc.subjectHelmintespt_PT
dc.subjectLobopt_PT
dc.subjectParasitaspt_PT
dc.titleStudy of gastrointestinal parasites in the iberian wolf (Canis lupus signatus)pt_PT
dc.typemaster thesis
dspace.entity.typePublication
rcaap.rightsopenAccesspt_PT
rcaap.typemasterThesispt_PT

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