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Avaliação in vitro da microinfiltração de adesivo experimental
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Abstract(s)
Objetivo: O presente estudo visa, numa amostra de 20 dentes humanos, o comportamento de um adesivo em relação à microinfiltração entre o adesivo e o esmalte, comparando diferentes protocolos de aplicação e tempos de armazenamento.
Materiais e Métodos: Numa amostra de 20 molares hígidos, com tecidos periodontais remanescentes, removidos por curetas Gracey (ASA Dental™), foram divididos aleatoriamente em 4 grupos de 5 dentes e conservados numa solução de timol a 4ºC. Para garantir a integridade das amostras, todas as superfícies radiculares receberam uma camada protetora de verniz. Foram selecionados 2 grupos, onde o esmalte foi
condicionado com ácido poliacrílico a 20% (3M ESPE) durante 1 minuto previamente à aplicação do adesivo, os grupos 1A e 3 A. Outros 2 grupos o adesivo foi aplicado diretamente sobre o esmalte intacto, os grupos 2 e 4. Posteriormente, todos os grupos foram colocados em água destilada e armazenados numa estufa de incubação a 37ºC (Memmert INE400, Memmert, Germany), sendo retirados em diferentes alturas. Após 24
horas, os grupos 1A e 2, foram imersos em fucsina básica e, recolocados na estufa durante 24 horas. Já, os grupos 3A e 4, permaneceram em água destilada e em estufa por 30 dias antes de serem submetidos ao mesmo processo de coloração. Todos os dentes foram lavados com água para que pudessem ser removidos excessos de fucsina e secos para incorporação em resina acrílica (ClaroCit Kit™, Struers). Os dentes foram seccionados no sentido vestíbulo-lingual através de um micrótomo de tecidos duros, em cortes com
espessura de 1 mm que apresentassem em simultâneo a presença de adesivo e esmalte. A análise da microinfiltração, foi realizada por observação dos cortes através de um esteromicroscópio (SMZ800N, Nikon, Japão) com ampliação de 10x, que classificou os padrões de infiltração em 3 níveis distintos.
Resultados: O grupo que apresentou maior microinfiltração foi o grupo 2, contrariamente, o grupo 3A foi o que apresentou menores níveis de microinfiltração.
Conclusão: A 1 mês, com condicionamento ácido prévio foram apresentados os melhores resultados. Confirmando as hipóteses alternativas e rejeitando as hipóteses nulas.
Objective: The present study aims, in a sample of 20 human teeth, the behavior of an adhesive in relation to microleakage between the adhesive and the enamel, comparing different application protocols and storage times. Materials and Methods: A sample of 20 healthy molars with no remaining periodontal tissue, removed with Gracey curettes (ASA Dental™), were randomly divided into 4 groups of 5 teeth and stored in a thymol solution at 4ºC. To ensure the integrity of the samples, all root surfaces were coated with a protective layer of varnish. Two groups were selected where the enamel would be conditioned with 20% polyacrylic acid (3M ESPE) for 1 minute prior to adhesive application, groups 1A and 3A, as well as two groups where the adhesive would be applied directly to the intact enamel, groups 2 and 4. The acidconditioned groups were rinsed with water and dried with a jet of air after the conditioning time. Subsequently, all groups were placed in distilled water and stored in an incubation oven at 37°C (Memmert INE400, Memmert, Germany), being removed at different times. After 24 hours, groups 1A and 2 were immersed in basic fuchsin and returned to the oven for 24 hours. Groups 3A and 4 remained in distilled water and in the oven for 30 days before undergoing the same staining process. All teeth were washed with water to remove excess fuchsin and dried for incorporation into acrylic resin. The teeth were sectioned in the vestibular-lingual direction using a hard tissue microtome, in 1 mm thick sections that simultaneously presented the presence of adhesive and enamel. Microleakage analysis was performed by observing the sections through a stereomicroscope (SMZ800N, Nikon, Japan) at 10x magnification, which classified the leakage patterns into three distinct levels. Results: Group 2 showed the highest microleakage, while group 3A showed the lowest levels of microleakage. Conclusion: At 1 month, the best results were obtained with prior acid conditioning. This confirmed the alternative hypotheses and rejected the null hypotheses.
Objective: The present study aims, in a sample of 20 human teeth, the behavior of an adhesive in relation to microleakage between the adhesive and the enamel, comparing different application protocols and storage times. Materials and Methods: A sample of 20 healthy molars with no remaining periodontal tissue, removed with Gracey curettes (ASA Dental™), were randomly divided into 4 groups of 5 teeth and stored in a thymol solution at 4ºC. To ensure the integrity of the samples, all root surfaces were coated with a protective layer of varnish. Two groups were selected where the enamel would be conditioned with 20% polyacrylic acid (3M ESPE) for 1 minute prior to adhesive application, groups 1A and 3A, as well as two groups where the adhesive would be applied directly to the intact enamel, groups 2 and 4. The acidconditioned groups were rinsed with water and dried with a jet of air after the conditioning time. Subsequently, all groups were placed in distilled water and stored in an incubation oven at 37°C (Memmert INE400, Memmert, Germany), being removed at different times. After 24 hours, groups 1A and 2 were immersed in basic fuchsin and returned to the oven for 24 hours. Groups 3A and 4 remained in distilled water and in the oven for 30 days before undergoing the same staining process. All teeth were washed with water to remove excess fuchsin and dried for incorporation into acrylic resin. The teeth were sectioned in the vestibular-lingual direction using a hard tissue microtome, in 1 mm thick sections that simultaneously presented the presence of adhesive and enamel. Microleakage analysis was performed by observing the sections through a stereomicroscope (SMZ800N, Nikon, Japan) at 10x magnification, which classified the leakage patterns into three distinct levels. Results: Group 2 showed the highest microleakage, while group 3A showed the lowest levels of microleakage. Conclusion: At 1 month, the best results were obtained with prior acid conditioning. This confirmed the alternative hypotheses and rejected the null hypotheses.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Adesivo Esmalte Microinfiltração Selante