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Publication
Diagnóstico laboratorial da infeção por SARS-CoV-2
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| 2.54 MB | Adobe PDF |
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Advisor(s)
Abstract(s)
A doença infeciosa respiratória COVID-19 causada pelo novo vírus SARS-CoV-2, tornou-se, a nível internacional uma emergência de saúde pública. A pandemia decorrente da elevada transmissibilidade e a rápida disseminação do vírus causou uma crise humanitária com impactos sociais e económicos em todo o mundo. Inicialmente os sintomas são febre, tosse, fadiga e congestão nasal, mas podem progredir rapidamente para estados de saúde mais críticos tais como a síndrome respiratória aguda severa e a morte. Para além da diversidade de sintomas relatados por pacientes infetados, existem indivíduos que não apresentam qualquer sintomatologia. Desta forma torna-se fulcral, a deteção precoce da infeção viral ativa para o controlo desta pandemia. Para responder a esta necessidade, foram desenvolvidos diversos testes de diagnóstico laboratorial, com diferentes características. Em Portugal, a deteção da infeção aguda por SARS-COV-2 é efetuada com recurso aos testes de diagnóstico laboratorial, RT-PCR, RT-LAMP, TMAAptimaTM e o teste rápido de antigénio.
Esta monografia tem como objetivo sintetizar a informação existente em relação aos vários testes laboratoriais, de forma a esclarecer a adequabilidade de utilização de cada um deles e permitir a aplicação do método mais adequado às diferentes situações no terreno.
The infectious respiratory disease, COVID-19, caused by the new SARS-CoV-2, has become an international public health emergency. The pandemic resulting from the high transmissibility and rapid spread of the virus caused a humanitarian crisis with social and economic impacts around the world. Initially, the symptoms are similar to those of a common flu such as fever, cough, fatigue and nasal congestion, but they can quickly progress to more serious health conditions such as severe acute respiratory syndrome and even death. In addition to the diversity of symptoms reported by infected patients, there are individuals who do not present any symptoms at all. Thus, the early detection of active viral infection becomes crucial for the control of this pandemic. In order to respond to this need, several laboratory diagnostic tests were developed, with different characteristics. In Portugal, the detection of acute SARS-COV-2 infection is performed using laboratory diagnostic tests, RT-PCR, RT-LAMP, TMA-AptimaTM and the rapid antigen test. This monograph aims to synthesize the existing information in relation to the various laboratory tests, in order to clarify the suitability of using each one of them and allow the application of the most appropriate method to different situations in the field.
The infectious respiratory disease, COVID-19, caused by the new SARS-CoV-2, has become an international public health emergency. The pandemic resulting from the high transmissibility and rapid spread of the virus caused a humanitarian crisis with social and economic impacts around the world. Initially, the symptoms are similar to those of a common flu such as fever, cough, fatigue and nasal congestion, but they can quickly progress to more serious health conditions such as severe acute respiratory syndrome and even death. In addition to the diversity of symptoms reported by infected patients, there are individuals who do not present any symptoms at all. Thus, the early detection of active viral infection becomes crucial for the control of this pandemic. In order to respond to this need, several laboratory diagnostic tests were developed, with different characteristics. In Portugal, the detection of acute SARS-COV-2 infection is performed using laboratory diagnostic tests, RT-PCR, RT-LAMP, TMA-AptimaTM and the rapid antigen test. This monograph aims to synthesize the existing information in relation to the various laboratory tests, in order to clarify the suitability of using each one of them and allow the application of the most appropriate method to different situations in the field.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
SARS-CoV-2 RT-PCR RT-LAMP TMA- AptimaTM TrAg