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LC-MS/MS-MS3 for determination and quantification of ∆9-tetrahydrocannabinol and metabolites in blood samples

2022-09Conference object Open access
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dc.contributor.authorProença, Paula
dc.contributor.authorMartinho, Beatriz
dc.contributor.authorTeixeira, Helena
dc.contributor.authorMonteiro, Carla
dc.contributor.authorSimões, Susana
dc.contributor.authorFranco, João
dc.contributor.authorCorte Real, F.
dc.date.accessioned2022-09-14T16:21:02Z
dc.date.available2023-08-01T00:30:24Z
dc.date.issued2022-09
dc.descriptionPoster apresentado no 59th meeting of TIAFT & 30th meeting of SFTA, Versailles (France), 5-8 Setembro 2022pt_PT
dc.description.abstractDue to the high prevalence of cannabinoids in forensic toxicology, it is crucial to have an efficient method that allows the use of a small sample amount and that requires a minimal sample preparation, for the determination and quantification of low concentrations. A simple, highly selective and high throughput liquid chromatography tandem mass spectrometry method (LC-MS/MS-MS3) was developed for the determination and quantification of ∆9-tetrahydrocannabinol (THC), 11-hydroxy-∆9-THC (THC-OH) and 11-nor-∆9-THC-9-carboxylic acid (THC-COOH) in blood samples. Chromatographic analysis was preceded by a protein precipitation of 0.1 mL of blood samples with acetonitrile, then THC, THC-OH, THC-COOH and deuterated internal standards were separated on an Acquity UPLC® HSS T3 (100 mm x 2.1 mm i.d., 1.8 mm) reversed-phase column using a gradient elution with 2 mM aqueous ammonium formate (0.1% formic acid) and methanol, at a flow rate of 0.4 mL/min, and with a run time of 10 min. For MS/MS-MS3 analysis, a SCIEX QTRAP® 6500+ linear ion trap triple quadrupole mass spectrometer was used via electrospray ionization (ESI), operated in multiple reaction mode (MRM) and linear ion trap mode (MS3). The method was validated in accordance with international accepted criteria and guidelines. The method was selective and linear between 0.5-100 ng/mL (r2>0.995). The lower limits of quantification (LLOQ) corresponded to the lowest concentrations used for the calibration curves. The coefficients of variation obtained for accuracy and precision were less than 15%. The mean recoveries were between 88.0-101.4% for the studied concentration levels (1 ng/mL, 5 ng/mL and 50 ng/mL). No significant interfering compounds, matrix effects or carryover were observed. The validated method provides a sensitive, efficient and robust procedure for the quantitation of cannabinoids in blood using LC–MS/MS-MS3 and a sample volume of 0.1 mL. This work is also a proof of concept for using LC-MS3 technique to determine drugs in biological samples.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/41816
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectCannabinoidspt_PT
dc.subjectLC-MS/MS-MS3pt_PT
dc.subjectWhole bloodpt_PT
dc.subjectProtein precipitationpt_PT
dc.titleLC-MS/MS-MS3 for determination and quantification of ∆9-tetrahydrocannabinol and metabolites in blood samplespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceVersailles, Françapt_PT
oaire.citation.title59th meeting of TIAFT & 30th meeting of SFTApt_PT
person.familyNameProença
person.familyNameTeixeira
person.familyNameMonteiro
person.familyNameSimões
person.familyNameCorte Real Gonçalves
person.givenNamePaula
person.givenNameHelena M.
person.givenNameCarla
person.givenNameSusana
person.givenNameFrancisco
person.identifier.ciencia-id4214-85EB-E58C
person.identifier.ciencia-id0B18-918A-80ED
person.identifier.orcid0000-0002-1642-636X
person.identifier.orcid0000-0001-7570-5521
person.identifier.orcid0000-0001-6665-315X
person.identifier.orcid0000-0002-9457-1546
person.identifier.orcid0000-0003-1495-9362
person.identifier.scopus-author-id7003981256
person.identifier.scopus-author-id6603582554
rcaap.embargofctPossível publicaçãopt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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