A carregar...
Publicação
Capacidade de predição de periodontite de parâmetros químicos habitualmente avaliados na urina tipo II (SG, CET, ERY/Hb, BIL e Glu) : estudo piloto em amostra oral
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 1.97 MB | Adobe PDF |
Autores
Orientador(es)
Resumo(s)
Introdução: A periodontite é uma doença inflamatória crónica que afeta os tecidos de suporte dentário, sendo uma das condições orais mais prevalentes a nível global. O seu diagnóstico convencional requer avaliação clínica e radiográfica realizada por profissionais especializados, limitando a aplicabilidade em rastreios comunitários. A utilização de biomarcadores nos fluidos orais tem sido explorada como alternativa para o desenvolvimento de ferramentas de triagem rápidas e não invasivas.
Objetivo: Avaliar a capacidade preditiva de cinco parâmetros químicos de tiras reagentes multiparamétricas de urinálise (SG, CET, ERY/Hb, BIL e GLU) aplicados em amostras de bochecho com soro fisiológico para a identificação de periodontite.
Materiais e Métodos: Estudo observacional transversal piloto realizado na Clínica de Medicina Dentária do Instituto Universitário Egas Moniz, entre março e maio de 2026. Cada participante realizou um bochecho com 10 mL de soro fisiológico, analisado com tiras reagentes (Gima Urine Screen 10, Itália). A avaliação periodontal foi realizada por quatro examinadores calibrados através de exame periodontal completo. A associação com o estado periodontal foi avaliada por regressão logística multivariável e curva ROC.
Resultados: Dos 500 indivíduos recrutados, 438 constituíram a amostra final, 288 (65,7%) não apresentaram periodontite e 150 (34,3%) foram diagnosticados com periodontite. Observou-se associação significativa entre leucócitos orais e periodontite (OR=1.99; IC95%: 1.47–2.70; p<0.001) e entre eritrócitos/hemoglobina e periodontite (OR=1.61; IC95%: 1.03–2.50; p=0.035). Os restantes parâmetros não demonstraram associação significativa. O modelo apresentou uma AUC de 0.677.
Conclusão: Os leucócitos e eritrócitos/hemoglobina avaliados em amostras de bochecho demonstraram associação significativa com periodontite, refletindo a resposta inflamatória neutrofílica e a hemorragia gengival. Embora a capacidade discriminativa seja moderada, os resultados suportam a exploração destas tiras como componente de ferramentas de triagem point-of-care em estudos futuros.
Introduction: Periodontitis is a chronic inflammatory disease affecting tooth-supporting tissues and one of the most prevalent oral conditions worldwide. Its conventional diagnosis requires specialized clinical assessment, limiting applicability in community screening settings. The use of biomarkers in oral fluids has been explored as an alternative for developing rapid and non-invasive screening tools. Objective: To evaluate the predictive capacity of five chemical parameters of multiparametric urinalysis reagent strips (SG, KET, ERY/Hb, BIL and GLU) applied to saline mouth rinse samples for the identification of periodontitis. Materials and Methods: A cross-sectional observational pilot study was conducted at the Dental Clinic Instituto Universitário Egas Moniz between March and May 2026. Each participant performed a 30 second mouth rinse with 10 mL of saline solution, analysed using reagent strips (Gima Urine Screen 10, Italy). Periodontal assessment was performed by four calibrated examiners through full-mouth examination. The association whit periodontal status was evaluated using multivariable logistic regression and ROC curve analysis. Results: Of 500 recruited individuals, 438 compromised the final sample, 288 (65,7%) didn’t present periodontitis and 150 (34,3) were diagnosed with periodontitis. A significant association was observed between oral leukocytes and periodontitis (OR=1.99; 95%CI:1.47-2.79; p<0.001) and between erythrocytes/hemoglobin and periodontitis (OR=1.61<; 95%CI:1.03-2.50; p=0.035). The remaining parameters chowed no significant associations. The model yielded an AUC of 0.677. Conclusion: Leukocyte and erythrocyte/hemoglobin parameters assessed in saline mouth rinse samples demonstrated significant association with periodontitis, reflecting the neutrophic inflammatory response and gingival. Although discriminative capacity is moderate these findings support the exploration of reagent strips as a component of point-of-care screening tools in future studies.
Introduction: Periodontitis is a chronic inflammatory disease affecting tooth-supporting tissues and one of the most prevalent oral conditions worldwide. Its conventional diagnosis requires specialized clinical assessment, limiting applicability in community screening settings. The use of biomarkers in oral fluids has been explored as an alternative for developing rapid and non-invasive screening tools. Objective: To evaluate the predictive capacity of five chemical parameters of multiparametric urinalysis reagent strips (SG, KET, ERY/Hb, BIL and GLU) applied to saline mouth rinse samples for the identification of periodontitis. Materials and Methods: A cross-sectional observational pilot study was conducted at the Dental Clinic Instituto Universitário Egas Moniz between March and May 2026. Each participant performed a 30 second mouth rinse with 10 mL of saline solution, analysed using reagent strips (Gima Urine Screen 10, Italy). Periodontal assessment was performed by four calibrated examiners through full-mouth examination. The association whit periodontal status was evaluated using multivariable logistic regression and ROC curve analysis. Results: Of 500 recruited individuals, 438 compromised the final sample, 288 (65,7%) didn’t present periodontitis and 150 (34,3) were diagnosed with periodontitis. A significant association was observed between oral leukocytes and periodontitis (OR=1.99; 95%CI:1.47-2.79; p<0.001) and between erythrocytes/hemoglobin and periodontitis (OR=1.61<; 95%CI:1.03-2.50; p=0.035). The remaining parameters chowed no significant associations. The model yielded an AUC of 0.677. Conclusion: Leukocyte and erythrocyte/hemoglobin parameters assessed in saline mouth rinse samples demonstrated significant association with periodontitis, reflecting the neutrophic inflammatory response and gingival. Although discriminative capacity is moderate these findings support the exploration of reagent strips as a component of point-of-care screening tools in future studies.
Descrição
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Palavras-chave
Periodontite Biomarcadores Saliva Gima