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Avaliação in vitro da microinfiltração de adesivo experimental
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Advisor(s)
Abstract(s)
Objetivo: Avaliar a microinfiltração de um adesivo em fase experimental, T1, aplicado como um selante, em dentes humanos.
Materiais e métodos: 20 terceiros molares humanos hígidos e sem contactos oclusais foram usados. Todos os dentes foram desinfetados com peróxido de hidrogénio, curetados os tecidos periodontais aderentes e armazenados numa solução de timol a 4ºC. Foram divididos aleatoriamente em 4 grupos de 5 dentes cada. As raízes de todos os dentes foram seladas com verniz. Nos grupos I e III não foi feito condicionamento ácido e o adesivo T1 foi aplicado diretamente nas fissuras. Nos grupos IIA e IVA foi feito condicionamento ácido com ácido ortofosfórico a 37% (3M ESPE) durante 30 segundos, seguido de lavagem com água corrente durante 30 segundos e secagem, de seguida o adesivo T1 foi aplicado. Os grupos I e IIA foram armazenados em água destilada numa estufa de incubação a 37ºC (Memmert INE400, Memmert, Germany), durante 24 horas. Os grupos III e IVA foram armazenados na mesma estufa por um período de 1 mês. De seguida, os dentes foram imersos numa solução de fucsina básica durante 24 horas e lavados com água corrente para remover o excesso de corante, secados e incluídos numa resina acrília transparente autopolimerizável (Clarocit kit, Struers, Denmark). As amostras foram seccionadas longitudinalmente no sentido vestíbulo-lingual com uma espessura de 1 mm e para cada dente foram selecionados 3 cortes. A microinfiltração foi avaliada com ampliação de 20x num estereomicroscópio (SMZ800N, Nikon, Japan) e classificados em 3 categorias: sem microinfiltração, microinfiltração média e microinfiltração total.
Resultados: O grupo III foi o que apresentou maior grau de microinfiltração, ao contrário do grupo I que foi o que apresentou menor percentagem de microinfiltração.
Conclusão: Às 24 horas o tratamento sem condicionamento ácido apresenta melhores resultados enquanto a 1 mês não há diferença significativa entre o tratamento com e sem condicionamento ácido. Aceitam-se as hipóteses alternativas e rejeitam-se as hipóteses nulas, havendo necessidade de reformular o adesivo futuramente.
Objective: This study aims to evaluate the microleakage of an experimental adhesive, T1, used as a sealant in human teeth. Materials and methods: 20 sound human third molars without oclusal contacts, were used. All theeth were cleaned of periodontal tissues with curettes and stored in a thymol solution at 4ºC.They were randomly divided into 4 groups of five theeth each. The roots were sealed with varnish. In group I and III, no acid etching were performed and the T1 adhesive were applied along the fissures. In group IIA and IVA, an 37% orthophosphoric acid (3M ESPE) was applied for 30 seconds, followed by water rising for 30 seconds and drying, then the T1 adhesive was applied. Group I and IIA were placed in distilled water and stored in an incubator at 37ºC (Memmert INE400, Memmert, Germany) for 24 hours. For group III and IVA they were stored in the same incubator for 1 month. Then the teeth were immersed in basic fuchsin for 24 hours.The samples were washed with running water to remove the excess dye, dried and immersed in a transparente self-polymerizing acrylic resin (Clarocit kit, Struers, Denmark). The samples were sectioned longitudinally in buccolingual direction to obtain 1 mm slices and for each tooth 3 slices were selected. The microleakage was evaluated using na 20x magnification under a stereomicroscope (SMZ800N, Nikon, Japan) and classified in three categories: no microleakage, medium microleakage and full microleakage. Results: Group III presented the highest degree of microleakage as opposed to group I which had the lowest percentagem of microleakage. Conclusion: At 24 hours, the treatment without acid etching shows better results, while after 1 month there is no significant difference between the treatments with and without acid etching. The alternative hypotheses are accepted, and the null hypotheses are rejected, indicating the need to reformulate the adhesive in the future.
Objective: This study aims to evaluate the microleakage of an experimental adhesive, T1, used as a sealant in human teeth. Materials and methods: 20 sound human third molars without oclusal contacts, were used. All theeth were cleaned of periodontal tissues with curettes and stored in a thymol solution at 4ºC.They were randomly divided into 4 groups of five theeth each. The roots were sealed with varnish. In group I and III, no acid etching were performed and the T1 adhesive were applied along the fissures. In group IIA and IVA, an 37% orthophosphoric acid (3M ESPE) was applied for 30 seconds, followed by water rising for 30 seconds and drying, then the T1 adhesive was applied. Group I and IIA were placed in distilled water and stored in an incubator at 37ºC (Memmert INE400, Memmert, Germany) for 24 hours. For group III and IVA they were stored in the same incubator for 1 month. Then the teeth were immersed in basic fuchsin for 24 hours.The samples were washed with running water to remove the excess dye, dried and immersed in a transparente self-polymerizing acrylic resin (Clarocit kit, Struers, Denmark). The samples were sectioned longitudinally in buccolingual direction to obtain 1 mm slices and for each tooth 3 slices were selected. The microleakage was evaluated using na 20x magnification under a stereomicroscope (SMZ800N, Nikon, Japan) and classified in three categories: no microleakage, medium microleakage and full microleakage. Results: Group III presented the highest degree of microleakage as opposed to group I which had the lowest percentagem of microleakage. Conclusion: At 24 hours, the treatment without acid etching shows better results, while after 1 month there is no significant difference between the treatments with and without acid etching. The alternative hypotheses are accepted, and the null hypotheses are rejected, indicating the need to reformulate the adhesive in the future.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Adesivos Fissuras Microinfiltração Selamento