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Differentiation of selegiline metabolites and illicit methamphetamine and amphetamine use in elderly postmortem cases: Insights from LC-MS/MS and immunoassay techniques

2024-09Other Embargoed access
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dc.contributor.authorCosta, Suzel
dc.contributor.authorRosendo, Luana M.
dc.contributor.authorBarroso, Mário
dc.contributor.authorFranco, João Miguel
dc.date.accessioned2025-01-30T17:25:07Z
dc.date.embargo2026-09
dc.date.issued2024-09
dc.descriptionComunicação Oral realizada nas IV Jornadas Ibéricas de Toxicologia, 26-27 setembro de 2024, Covilhã, Portugalpt_PT
dc.description.abstractAbstract: Introduction: In forensic toxicology, distinguishing between pharmaceutical metabolites and illicit substances is crucial for accurate case interpretation. This study explores two post-mortem cases involving elderly individuals where methamphetamine and amphetamine were detected, raising initial concerns about illicit drug use. Aims: To demonstrate the application of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immunoassay techniques in differentiating between selegiline metabolites and illicit methamphetamine and amphetamine. Methods: Blood samples from three deceased elderly individuals (ages 91 and 89) were analyzed using LC-MS/MS for the quantitative detection of selegiline and metabolites. Initial screening revealed methamphetamine and amphetamine levels suggestive of potential illicit use. Immunoassay testing for dextro isomers (those associated with drug abuse) was subsequently performed to differentiate between isomers. Results and Discussion: LC-MS/MS analysis confirmed selegiline in both cases, with methamphetamine and amphetamine detected. Immunoassay results were negative for dextro isomers, indicating that the detected substances were L-isomers, which is consistent with selegiline metabolism rather than illicit use. Conclusion: This study highlights the critical role of combining LC-MS/MS and immunoassay techniques in forensic toxicology. The negative immunoassay results for dextro isomers clarified that the methamphetamine and amphetamine present were metabolic byproducts of selegiline therapy. Accurate differentiation between therapeutic and illicit substances is essential to avoid misinterpretation, particularly in elderly individuals, where illicit drug use is infrequent. These findings emphasize the need for comprehensive analytical approaches in forensic investigations to ensure accurate and reliable results.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/54093
dc.language.isoporpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSelegilinept_PT
dc.subjectLC-MS/MSpt_PT
dc.subjectPost-mortem analysispt_PT
dc.subjectImmunoassaypt_PT
dc.subjectMetabolitespt_PT
dc.titleDifferentiation of selegiline metabolites and illicit methamphetamine and amphetamine use in elderly postmortem cases: Insights from LC-MS/MS and immunoassay techniquespt_PT
dc.typeother
dspace.entity.typePublication
oaire.citation.conferencePlaceCovilhã, Portugalpt_PT
oaire.citation.titleIV Jornadas Ibéricas de Toxicologiapt_PT
person.familyNameCosta
person.familyNameda Silva Rosendo
person.familyNameBarroso
person.familyNameFranco
person.givenNameSuzel
person.givenNameLuana May
person.givenNameMário
person.givenNameJoão
person.identifier.ciencia-idF611-6D58-E4C2
person.identifier.ciencia-id4E12-377B-7569
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.orcid0009-0002-1268-8052
person.identifier.orcid0000-0002-0461-8540
person.identifier.orcid0000-0001-8848-2734
person.identifier.orcid0000-0002-3268-3841
person.identifier.scopus-author-id35329243100
rcaap.rightsembargoedAccesspt_PT
rcaap.typeotherpt_PT
relation.isAuthorOfPublication8592c807-a8a5-4408-90d5-b32ceaf6b363
relation.isAuthorOfPublication52a4b3fa-fd99-413d-baab-ab4119bd46e9
relation.isAuthorOfPublication294b555e-48c4-4013-b981-b8917400c56c
relation.isAuthorOfPublication045c4125-1313-445f-b05f-b7f204886052
relation.isAuthorOfPublication.latestForDiscovery8592c807-a8a5-4408-90d5-b32ceaf6b363

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