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Propriedades superficiais da dentina tratada com plasma não térmico atmosférico : avaliação do ângulo de contacto e análise topográfica
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Objetivo: Com o presente estudo laboratorial, pretendeu-se avaliar o efeito do Plasma não térmico atmosférico (PNTA) na superfície da dentina, sendo analisada, para isso, a influência do tratamento de superfície (condicionamento com ácido ortofosfórico a 37% ou aplicação de PNTA) na molhabilidade da dentina. Foi ainda examinada a topografia da superfície dentinária antes e após os dois tratamentos.
Materiais e Métodos: Para a primeira fase do estudo foram utilizados 10 dentes humanos hígidos. Os dentes foram seccionados num micrótomo de tecidos duros, de forma a obter um disco de dentina por dente. Foram criados dois grupos experimentais (n=5) de acordo com a preparação da superfície da dentina. Num dos grupos, foi aplicado PNTA ; no outro, ácido fosfórico a 37%. A influência na molhabilidade foi avaliada
através da medição do ângulo de contacto antes e após a preparação do substrato dentinário, com recurso a um espectro-goniómetro. Numa segunda fase de estudo, foram utilizados 4 dentes humanos hígidos, distribuídos pelos tratamentos (n=2). A avaliação da topografia da superfície da dentina foi feita com recurso a um microscópio de força atómica.
Resultados: Observou-se uma diminuição significativa do ângulo de contacto após o tratamento em ambos os grupos (p=0,005). Contudo, nenhuma das duas abordagens demonstrou ser significativamente mais eficaz do que a outra na modificação da molhabilidade (p > 0,05). Adicionalmente, constatou-se que o tratamento com PNTA não provoca alterações na topografia da superfície dentinária, ao passo que o condicionamento com ácido ortofosfórico a 37% induz modificações visíveis na estrutura e aumenta a rugosidade.
Conclusões: Tanto o ácido ortofosfórico a 37% como o PNTA demonstraram ser eficazes na melhoria da molhabilidade da dentina. No entanto, enquanto o tratamento com PNTA preserva a topografia da superfície dentinária, o condicionamento com ácido ortofosfórico a 37% provoca alterações estruturais evidentes.
Purpose: In this laboratory study, the aim was to evaluate the effect of Atmospheric Non-Thermal Plasma (ANTP) on the dentin surface, by analyzing the influence of surface treatment (conditioning with 37% phosphoric acid or application of ANTP) on dentin wettability. The topography of the dentin surface was also examined before and after both treatments. Materials and Methods: In the first phase of the study, 10 sound human teeth were used. The teeth were sectioned using a hard tissue microtome to obtain one dentin disc per tooth. Two experimental groups (n=5) were created according to the dentin surface preparation. In one group, NATP was applied; in the other, 37% phosphoric acid. The influence on wettability was assessed by measuring the contact angle before and after preparation of the dentin substrate, using a spectro-goniometer. In a second phase of the study, 4 sound human teeth were used, distributed between the treatments (n=2). The dentin surface topography was evaluated using an atomic force microscope. Results: A significant decrease in the contact angle was observed after treatment in both groups (p=0.005). However, neither approach proved to be significantly more effective than the other in modifying wettability (p > 0.05). Additionally, it was found that treatment with NATP does not cause changes in the topography of the dentin surface, whereas conditioning with 37% phosphoric acid induces visible structural modifications and increases surface roughness. Conclusions: Both 37% phosphoric acid and NATP proved effective in improving dentin wettability. However, while NATP treatment preserves the topography of the dentin surface, conditioning with 37% phosphoric acid causes evident structural changes.
Purpose: In this laboratory study, the aim was to evaluate the effect of Atmospheric Non-Thermal Plasma (ANTP) on the dentin surface, by analyzing the influence of surface treatment (conditioning with 37% phosphoric acid or application of ANTP) on dentin wettability. The topography of the dentin surface was also examined before and after both treatments. Materials and Methods: In the first phase of the study, 10 sound human teeth were used. The teeth were sectioned using a hard tissue microtome to obtain one dentin disc per tooth. Two experimental groups (n=5) were created according to the dentin surface preparation. In one group, NATP was applied; in the other, 37% phosphoric acid. The influence on wettability was assessed by measuring the contact angle before and after preparation of the dentin substrate, using a spectro-goniometer. In a second phase of the study, 4 sound human teeth were used, distributed between the treatments (n=2). The dentin surface topography was evaluated using an atomic force microscope. Results: A significant decrease in the contact angle was observed after treatment in both groups (p=0.005). However, neither approach proved to be significantly more effective than the other in modifying wettability (p > 0.05). Additionally, it was found that treatment with NATP does not cause changes in the topography of the dentin surface, whereas conditioning with 37% phosphoric acid induces visible structural modifications and increases surface roughness. Conclusions: Both 37% phosphoric acid and NATP proved effective in improving dentin wettability. However, while NATP treatment preserves the topography of the dentin surface, conditioning with 37% phosphoric acid causes evident structural changes.
Descrição
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Palavras-chave
Plasma não térmico atmosférico Ácido fosfórico Molhabilidade Topografia