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Production of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) by Burkholderia sacchari using wheat straw hydrolysates and gamma-butyrolactone

dc.contributor.authorCesário, M. Teresa
dc.contributor.authorRaposo, Rodrigo S.
dc.contributor.authorAlmeida, M. Catarina M. D. de
dc.contributor.authorVan Keulen, Frederik
dc.contributor.authorFerreira, Bruno S.
dc.contributor.authorTelo, João P.
dc.contributor.authorFonseca, M. Manuela R. da
dc.date.accessioned2015-04-21T15:39:30Z
dc.date.available2015-04-21T15:39:30Z
dc.date.issued2014-11
dc.description“NOTICE: this is the author’s version of a work that was accepted for publication in International Journal of Biological Macromolecules. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Journal of Biological Macromolecules, Vol. 71, Special issue, (November 2014). DOI 10.1016/j.ijbiomac.2014.04.054 "
dc.description.abstract"Burkholderia sacchari DSM 17165 is able to grow and produce poly(3-hydroxybutyrate) both on hexoses and pentoses. In a previous study, wheat straw lignocellulosic hydrolysates (WSH) containing high C6 and C5 sugar concentrations were shown to be excellent carbon sources for P(3HB) production. Using a similar feeding strategy developed for P(3HB) production based on WSH, fedbatch cultures were developed aiming at the production of the copolymer P(3HB-co- 4HB) (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)) by B. sacchari. The ability of this strain to synthesize P(3HB-co-4HB) was first shown in shake flasks using gammabutyrolactone (GBL) as precursor of the 4HB units. Fed-batch cultures using glucose as carbon source (control) and GBL were developed to achieve high copolymer productivities and 4HB incorporations. The attained P(3HBco- 4HB) productivity and 4HB molar % were 0.7 g/(L·h) and 4.7 molar %, respectively. The 4HB incorporation was improved to 6.3 and 11.8 molar % by addition of 2 g/L propionic and acetic acid, respectively. When WSH were used as carbon source under the same feeding conditions, the values achieved were 0.5 g/(L·h) and 5.0 molar %, respectively. Burkholderia sacchari, a strain able to produce biopolymers based on xylose-rich lignocellulosic hydrolysates, is for the first time reported to produce P(3HB-co-4HB) using gamma butyrolactone as precursor."en
dc.description.sponsorshipThis work has received funding from the European Union 7th Framework Programme (FP7/2007-2013) under Grant Agreement number 246449 / Project: Bugworkers - New tailor-made PHB-based nanocomposites for high performance applications produced from environmentally friendly production routes.
dc.description.sponsorshipFinancial support: Fundação para a Ciência e Tecnologia, Portugal (fellowships SFRH/BPD/68587/2010 and SFRH/BPD/26678/2006).
dc.identifier.citationInt J Biol Macromol. 2014 Nov;71:59-67. doi: 10.1016/j.ijbiomac.2014.04.054en
dc.identifier.doi10.1016/j.ijbiomac.2014.04.054
dc.identifier.issn0141-8130
dc.identifier.urihttp://hdl.handle.net/10400.26/8457
dc.language.isoengen
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationNew tailor-made PHB-based nanocomposites for high performance applications produced from environmentally friendly production routes
dc.relationPRODUÇÃO DE POLIHIDROXIALCANOATOS UTILIZANDO SUBSTRATOS RESIDUAIS
dc.relationPRODUCTION OF POLY 3-HYDROXYBUTYRATE-CO- 4-HYDROXYBUTYRATE P 3HB-CO-4HB FROM LIGNOCELLULOSIC AGRO-INDUSTRIAL WASTES
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0141813014002815por
dc.subjectBurkholderia saccharien
dc.subjectPoly(3-hydroxybutyrate-co-4-hydroxybutyrate)en
dc.subjectWheat straw lignocellulosic hydrolysatesen
dc.titleProduction of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) by Burkholderia sacchari using wheat straw hydrolysates and gamma-butyrolactoneen
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNew tailor-made PHB-based nanocomposites for high performance applications produced from environmentally friendly production routes
oaire.awardTitlePRODUÇÃO DE POLIHIDROXIALCANOATOS UTILIZANDO SUBSTRATOS RESIDUAIS
oaire.awardTitlePRODUCTION OF POLY 3-HYDROXYBUTYRATE-CO- 4-HYDROXYBUTYRATE P 3HB-CO-4HB FROM LIGNOCELLULOSIC AGRO-INDUSTRIAL WASTES
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/246449/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F26678%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F68587%2F2010/PT
oaire.citation.endPage67por
oaire.citation.startPage59por
oaire.citation.titleInternational Journal of Biological Macromoleculesen
oaire.citation.volume71 (Special issue: Biodegradable biopolymers)en
oaire.fundingStreamFP7
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isProjectOfPublicatione88d1819-1f6d-4b1d-a864-ec2091b17e8a
relation.isProjectOfPublication54143930-0087-45be-ae76-b932ef0f1af1
relation.isProjectOfPublication8b03dfca-bdb8-448e-8333-cbd672ab33ee
relation.isProjectOfPublication.latestForDiscovery54143930-0087-45be-ae76-b932ef0f1af1

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