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Alterações fenotípicas nas células B normais em leucemia linfocítica crónica-B
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Abstract(s)
A Leucemia Linfocítica Crônica (LLC-B) é caracterizada pela acumulação monoclonal de linfócitos B CD5+ no sangue periférico, medula óssea e nos órgãos linfoides secundários. A imunofenotipagem é essencial para confirmar o diagnóstico. O conhecimento relativo das células B normais residuais da medula óssea destes doentes é no entanto escasso.
O objetivo deste trabalho foi quantificar a frequência das células B normais na medula óssea nos seus diferentes compartimentos maturativos, células imaturas, hematogónias, células maduras e células plasmáticas. Bem como, caracterizar
fenotipicamente as referidas subpopulações, em doentes com LLC-B e em casos normais. E comparar estes dados com as alterações genéticas mais frequentes nesta entidade.
Foram estudadas 43 amostras de aspirados medulares de doentes com LLC-B e o grupo controlo foi constituído por 10 amostras de aspirados medulares normais/ reativos.
Verificou-se uma diminuição da percentagem das células plasmáticas e células B maduras normais nos indivíduos com LLC-B quando comparado com o grupo controlo, após exclusão das células B da LLC-B no primeiro grupo. Na avaliação de cada compartimento maturativo também se observaram diferenças na expressão das moléculas estudadas, bem como, o mesmo ocorrendo dentro de subgrupos de LLC-B de acordo com as alterações genéticas presentes.
Após a realização deste trabalho verificou-se alterações numéricas e fenotípicas importantes nas células B normais residuais em doentes com LLC-B.
The chronic lymphocytic leukemia (CLL-B) is characterized by the accumulation of monoclonal CD5+ B lymphocytes in the blood, bone marrow and lymphoid tissues. Immunophenotyping is essential to confirm the diagnosis. The knowledge concerning the residual bone marrow normal B cells from these patients is still scarce. The aim of this study was to quantify the frequency of normal B cells in the bone marrow in its different maturational compartments, immature cells, hematogones, mature cells and plasma cells. Characterize phenotypically these subpopulations in CLL-B patients and in normal cases, and compare the obtained results among different CLL-B subgroups according to most common genetic abnormalities in this entity. In this study were evaluated 43 marrow aspirates from CLL-B patients and 10 normal bone marrow aspirates. There was a decrease in the percentage of plasma cells and mature B cells in CLL-B group when compared with the control group. We also found differences in the expression of several molecules on B cells from CLL-B group, some of them with a clear relation with the presence of a specific genetic abnormality. In this work it was verified numerical and phenotypic changes in residual normal bone marrow B cells from patients with CLL-B.
The chronic lymphocytic leukemia (CLL-B) is characterized by the accumulation of monoclonal CD5+ B lymphocytes in the blood, bone marrow and lymphoid tissues. Immunophenotyping is essential to confirm the diagnosis. The knowledge concerning the residual bone marrow normal B cells from these patients is still scarce. The aim of this study was to quantify the frequency of normal B cells in the bone marrow in its different maturational compartments, immature cells, hematogones, mature cells and plasma cells. Characterize phenotypically these subpopulations in CLL-B patients and in normal cases, and compare the obtained results among different CLL-B subgroups according to most common genetic abnormalities in this entity. In this study were evaluated 43 marrow aspirates from CLL-B patients and 10 normal bone marrow aspirates. There was a decrease in the percentage of plasma cells and mature B cells in CLL-B group when compared with the control group. We also found differences in the expression of several molecules on B cells from CLL-B group, some of them with a clear relation with the presence of a specific genetic abnormality. In this work it was verified numerical and phenotypic changes in residual normal bone marrow B cells from patients with CLL-B.
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Keywords
Leucemia linfóide Linfócitos B