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QUANTIFILER®TRIO DNA method performance in a collection of ancient samples

2015-07Conference object Open access
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dc.contributor.authorVieira- Silva, C.
dc.contributor.authorLopes, J.
dc.contributor.authorAfonso Costa, H.
dc.contributor.authorRibeiro, T.
dc.contributor.authorPorto, M.J.
dc.contributor.authorDias, M
dc.contributor.authorCunha, E
dc.contributor.authorAmorim, A,
dc.date.accessioned2022-07-27T16:30:06Z
dc.date.available2022-07-27T16:30:06Z
dc.date.issued2015-07
dc.descriptionTrabalho científico apresentado sob a forma de Poster no evento 9th ISABS Conference on Forensic Anthropologic Genetics and Mayo Clinicm Bol, Island of Brac, Croacia, 2015pt_PT
dc.description.abstractDuring the past few years significant progress has been made in solving technical challenges associated with STR profiling including the ability to analyze degraded DNA and low amounts of DNA. The result of these changes is that useful STR profiles can now be obtained from previously untypeable forensic DNA samples. Analysis of DNA from ancient material represents an important role in molecular anthropology, although there are many limitations concerning low DNA quantity and/or degraded DNA, and/or PCR inhibitors. These factors can make it difficult to decide whether to continue with STR analysis, which STR panel to use and how much DNA to add to PCR reaction. With all these constraints, DNA quantification represents an important tool to decide which method will follow in order to improve workflow and have good results in less time-consuming. The Quantifiler® Trio DNA method provides a quality index (QI) to detect the presence of degraded DNA along with PCR inhibitors.This guide allows the selection of the optimal short tandem repeat (STR) analysis chemistry (autosomal, or miniSTR) and streamlines the workflow while increasing downstream analysis success rates. In order to compare DNA quality from different extraction methods, samples from 46 exhumed Middle Ages individuals were extracted with modified phenol-chloroform method and also PrepFiler Express BTA™ method. DNA was quantified with Quantifiler® Trio DNA Quantification in an Applied Biosystems® 7500 Real-Time PCR System. Results were analyzed and allow us to point Quantifiler® Trio method as an important tool in pre-STR typing methods in ancient samplespt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/41486
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectForensic geneticspt_PT
dc.subjectAncient DNApt_PT
dc.subjectDNA quanficationpt_PT
dc.subjectQuantifiler Triopt_PT
dc.titleQUANTIFILER®TRIO DNA method performance in a collection of ancient samplespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceBol, Island of Brac, Croaciapt_PT
oaire.citation.title9th ISABS Conference on Forensic Anthropologic Genetics and Mayo Clinicpt_PT
person.familyNameda Silva
person.familyNameAfonso Costa
person.familyNameRibeiro
person.familyNameDias
person.familyNameCunha
person.familyNameAmorim
person.givenNameCláudia
person.givenNameHeloísa
person.givenNameTeresa
person.givenNameMário
person.givenNameEugénia
person.givenNameAntónio
person.identifierhttps://scholar.google.pt/citations?user=HSGHLA0AAAAJ&hl=en
person.identifier.ciencia-idD218-0D74-678B
person.identifier.ciencia-idE717-5464-E13B
person.identifier.orcid0000-0003-0373-7457
person.identifier.orcid0000-0002-7823-7687
person.identifier.orcid0000-0002-2903-0006
person.identifier.orcid0000-0002-5900-5899
person.identifier.orcid0000-0003-2998-371X
person.identifier.orcid0000-0002-7506-4426
person.identifier.ridJ-5725-2015
person.identifier.ridA-4414-2013
person.identifier.scopus-author-id9839086200
person.identifier.scopus-author-id24729171700
person.identifier.scopus-author-id26221673200
person.identifier.scopus-author-id190305008042
person.identifier.scopus-author-id35253426400
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublicatione75e9d2d-2282-46d8-a2cb-37b7edbb1156
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