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Aplicação do plasma não térmico atmosférico na dentisteria restauradora - um desafio promissor : estudo laboratorial
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Advisor(s)
Abstract(s)
Objetivos: Avaliar o efeito do CAP na resistência adesiva à dentina, por microtração (μTBS) após 24 h, utilizando o sistema adesivo Futurabond M+ (VOCO) com uma estratégia Self-Etch (SE). Avaliar a ultramorfologia e espessura da camada híbrida, através de microscopia eletrónica de varrimento (MEV) e microespectroscopia de Raman (μRaman).
Materiais e Métodos: Foram preparados vinte molares humanos íntegros e divididos aleatoriamente em dois grupos experimentais (n=10): Grupo Controlo – apenas aplicação de adesivo Futurabond M+, de acordo com indicações do fabricante para estratégia SE; Grupo CAP - aplicação de CAP por 60 s antes do Futurabond M+, modo SE. Após obtenção de uma fatia de dentina de cada dente e simulação da smear layer, realizou-se o procedimento restaurador de acordo com o grupo experimental, terminando com aplicação de resina composta Filtek Z250. Os dentes foram seccionados em palitos de ±1 mm² num micrótomo de tecidos duros para o teste de μTBS realizado numa máquina de testes universal (1 kN; 0,5 mm/min). As superfícies de falha foram examinadas e classificadas com recurso a um estereomicroscópio. Os dados foram analisados estatisticamente com One-Way ANOVA. Foram selecionados aleatoriamente dois palitos de grupo para análise com MEV e μRaman.
Resultados: Foram observadas diferenças estatisticamente significativas (p=0,026) entre os grupos, sendo que o CAP apresentou maior resistência adesiva em relação ao Controlo, com cerca de 32% de aumento nos valores obtidos, 33,44 + 10,43 MPa e 24,22 + 6,07 Mpa, respetivamente. A análise em MEV revelou uma interface adesiva aparentemente mais íntegra no grupo CAP, assim como mais espessa, facto avaliado por μRaman.
Conclusões: A aplicação de CAP, durante 60 s, melhorou a adesão do sistema adesivo Futurabond M+ à dentina, às 24h. Promoveu ainda a formação de uma interface adesiva aparentemente mais espessa e íntegra.
Purpose: To evaluate the impact of CAP on dentine bond strength through μTBS after 24 hours, using the Futurabond M+ adhesive system (VOCO) with a SE approach. Also, to evaluate the morphology and thickness of the hybrid layer using scanning electron microscopy (SEM) and micro-Raman spectroscopy analysis (μRaman) Materials and Methods: Twenty permanent molars were prepared and randomly divided into two experimental groups (n = 10): Group Control – application of Futurabond M+ adhesive exclusively, following the manufacturer's guidelines for the SE strategy and Group CAP – CAP application for 60 s preceding Futurabond M+ in SE mode. Following the exposure of the dentine surface and the emulation of the smear layer, the restorative procedure was conducted in accordance with the requirements of the experimental group, culminating in the application of Filtek Z250 composite resin. The teeth were sectioned into 1 mm² sticks using a hard tissue microtome for the μTBS test, which was performed using a universal testing machine (1 kN; 0.5 mm/min). Fracture surfaces of each stick were observed through a stereomicroscope, and failure types were categorised. Data underwent statistical analysis using one-way ANOVA. Additionally, one stick per group was randomly chosen for both SEM and μRaman. Results: Significant statistical differences (p = 0.026) were observed between the two groups. CAP exhibited higher bond strength compared to the Control, with an approximately 32% increase in the obtained values, 33,44 ± 10,43 MPa and 24,22 ± 6,07 MPa, respectively. The SEM analysis revealed a noticeably more homogeneous adhesive interface within the CAP group, coupled with an increased thickness, as confirmed by μRaman. Conclusions: A 60 s CAP application was found to enhance the dentine adhesion of Futurabond M+ after 24 hours. It additionally facilitated the establishment of a bonding interface which appeared to be simultaneously thicker and more cohesive.
Purpose: To evaluate the impact of CAP on dentine bond strength through μTBS after 24 hours, using the Futurabond M+ adhesive system (VOCO) with a SE approach. Also, to evaluate the morphology and thickness of the hybrid layer using scanning electron microscopy (SEM) and micro-Raman spectroscopy analysis (μRaman) Materials and Methods: Twenty permanent molars were prepared and randomly divided into two experimental groups (n = 10): Group Control – application of Futurabond M+ adhesive exclusively, following the manufacturer's guidelines for the SE strategy and Group CAP – CAP application for 60 s preceding Futurabond M+ in SE mode. Following the exposure of the dentine surface and the emulation of the smear layer, the restorative procedure was conducted in accordance with the requirements of the experimental group, culminating in the application of Filtek Z250 composite resin. The teeth were sectioned into 1 mm² sticks using a hard tissue microtome for the μTBS test, which was performed using a universal testing machine (1 kN; 0.5 mm/min). Fracture surfaces of each stick were observed through a stereomicroscope, and failure types were categorised. Data underwent statistical analysis using one-way ANOVA. Additionally, one stick per group was randomly chosen for both SEM and μRaman. Results: Significant statistical differences (p = 0.026) were observed between the two groups. CAP exhibited higher bond strength compared to the Control, with an approximately 32% increase in the obtained values, 33,44 ± 10,43 MPa and 24,22 ± 6,07 MPa, respectively. The SEM analysis revealed a noticeably more homogeneous adhesive interface within the CAP group, coupled with an increased thickness, as confirmed by μRaman. Conclusions: A 60 s CAP application was found to enhance the dentine adhesion of Futurabond M+ after 24 hours. It additionally facilitated the establishment of a bonding interface which appeared to be simultaneously thicker and more cohesive.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Plasma não térmico atmosférico Adesivos universais Adesão à dentina Teste de microtração