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Regulatory functions of sigma factors of RNA Polymerase SIGH and SIG E in Corynebacterium glutamicum
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The study presented in this document was developed in the laboratory of molecular genetics of bacteria from the Institute of Microbiology of the Czech Academy of Sciences. With the main objective of studying the structure of the promoters associated to the different sigma factors, the bacterium Corynebacterium glutamicum was used, a non-pathogenic bacterium widely used industrially in the production of amino acids. To this end were used four different promoters, two recognized by the sigma factor H and the other two by sigma factor E. To make it possible to obtain the strains are necessary for the development of the study, first it was necessary to proceed with the preparation of plasmids pEC-XT99A and the pEPRI who bore the different sigma factors and the different promoters, respectively, being then necessary to carry out the processing of cells with plasmids. The results of the activity of the promoters were obtained through fluorescence assays, through the pEPRI plasmid that contained the reporter gene that encodes for the green fluorescent protein (GFP).
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Palavras-chave
Corynebacterium glutamicum Transformation Sigma Factor Fluorescence Assay