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Publication
Development of a customized 3D membrane with therapeutic adjusted to the different stages of periodontal disease
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Abstract(s)
As doenças periodontais constituem um desafio significativo para a saúde, caracterizadas por
inflamação crónica e deterioração progressiva do periodonto. Embora os tratamentos atuais
se concentrem na remoção da placa bacteriana e na administração convencional de fármacos,
persistem limitações, como danos a células saudáveis. A cirurgia periodontal é uma alternativa
em casos graves, contudo, as membranas existentes não atendem adequadamente às
necessidades clínicas. Com isto, surge a motivação para apresentar uma solução inovadora
que emprega a tecnologia de impressão 3D para a criação de membranas gelatinosas
personalizadas, incorporando propriedades específicas benéficas.
O objetivo fundamental deste estudo consistiu em formular um hidrogel multifuncional,
integrando nanopartículas de Hidroxiapatite e Óxido de Grafeno, visando o tratamento de
doenças periodontais. Este hidrogel foi utilizado na técnica de impressão 3D com o intuito de
desenvolver uma membrana personalizada à base de gelatina, dotada de propriedades
antimicrobianas, anti-inflamatórias e osteoindutoras.
Os hidrogéis multifuncionais desenvolvidos demonstraram uma reologia adequada para
impressão 3D com 7,5% de GO e 2% de HAp na sua constituição. A estabilidade do hidrogel
não foi afetada pela adição de componentes. A confirmação de HAp-NPs na superfície do
hidrogel foi obtida e os testes de hidratação da membrana revelaram que as capacidades de
absorção de água eram dependentes da temperatura, onde os hidrogéis com GO
demonstraram um desempenho superior atribuído às suas interações específicas com as
moléculas de água. Além disso, os resultados dos testes de tração revelaram que a
incorporação do GO proporcionou melhores propriedades mecânicas. O processo de
impressão 3D foi bem sucedido, com medições de porosidade que provam a possibilidade de
produzir estruturas porosas com tamanhos de poros controlados.
Estes resultados conduziram ao desenvolvimento bem-sucedido de hidrogéis multimateriais
à base de gelatina. Estes hidrogéis exibiram propriedades reológicas favoráveis, estabilidade
estrutural, resistência mecânica e excelente capacidade para ser impresso recorrendo à
tecnologia de impressão 3D, assegurando resultados confiáveis e reprodutíveis. Essa eficácia
sugere que esses hidrogéis podem ser aplicados no contexto do tratamento de doenças
periodontais.
Periodontal disease poses a significant health challenge, leading to chronic inflammation and progressive deterioration of the periodontium. Current treatments focus on plaque removal and conventional drug delivery methods, but limitations exist, such as damage to healthy cells. Periodontal surgery is an option in severe cases, but available membranes do not meet clinical needs. This motivates the proposal of an innovative solution using 3D printing technology to create personalized gelled membranes with specific beneficial properties. The work aims to produce a multifunctional gelled hydrogel incorporating Hydroxyapatite nanoparticles and Graphene Oxide for treatment of periodontal disease. This hydrogel will be applied in 3D printing technology to create a personalized gelled membrane with antimicrobial, anti-inflammatory, and osteoinductive effects. The multifunctional hydrogels demonstrated optimized rheology for 3D printing with 7.5% of GO and 2% of HAp. The hydrogel's stability remained unaffected by the addition of components. Confirmation of HAp-NPs on the hydrogel surface was achieved and water stability tests revealed that water absorption capacities were temperature-dependent, with GO- containing hydrogels demonstrating superior performance attributed to their specific interactions with water molecules. Furthermore, the results of the tensile tests reveal that the incorporation of GO has led to improved mechanical properties. The 3D printing process was successful, with porosity measurements that demonstrate the possibility of producing porous structures with controlled pore sizes. These results proved the successful development of gelatin-based multimaterial hydrogels. These hydrogels demonstrated favorable rheological properties, structural stability, mechanical strength, and excellent 3D printing capabilities, guaranteeing reliable and reproducible results, suggesting that they can be applied in the treatment of periodontal diseases.
Periodontal disease poses a significant health challenge, leading to chronic inflammation and progressive deterioration of the periodontium. Current treatments focus on plaque removal and conventional drug delivery methods, but limitations exist, such as damage to healthy cells. Periodontal surgery is an option in severe cases, but available membranes do not meet clinical needs. This motivates the proposal of an innovative solution using 3D printing technology to create personalized gelled membranes with specific beneficial properties. The work aims to produce a multifunctional gelled hydrogel incorporating Hydroxyapatite nanoparticles and Graphene Oxide for treatment of periodontal disease. This hydrogel will be applied in 3D printing technology to create a personalized gelled membrane with antimicrobial, anti-inflammatory, and osteoinductive effects. The multifunctional hydrogels demonstrated optimized rheology for 3D printing with 7.5% of GO and 2% of HAp. The hydrogel's stability remained unaffected by the addition of components. Confirmation of HAp-NPs on the hydrogel surface was achieved and water stability tests revealed that water absorption capacities were temperature-dependent, with GO- containing hydrogels demonstrating superior performance attributed to their specific interactions with water molecules. Furthermore, the results of the tensile tests reveal that the incorporation of GO has led to improved mechanical properties. The 3D printing process was successful, with porosity measurements that demonstrate the possibility of producing porous structures with controlled pore sizes. These results proved the successful development of gelatin-based multimaterial hydrogels. These hydrogels demonstrated favorable rheological properties, structural stability, mechanical strength, and excellent 3D printing capabilities, guaranteeing reliable and reproducible results, suggesting that they can be applied in the treatment of periodontal diseases.
Description
Keywords
Doenças periodontais Impressão 3D Membrana personalizada Nanopartículas de Hidroxiapatite Óxido de Grafeno Periodontal disease 3D printing Personalized membrane Hydroxyapatite nanoparticles Graphene Oxide