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Publication
IDS com um novo sistema adesivo : caracterização morfológica
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Advisor(s)
Abstract(s)
Objetivo: Caracterizar e comparar morfologicamente a interface adesiva obtida com diferentes protocolos de Immediate Dentin Sealing (IDS) através de Microscopia Eletrónica de Varrimento (MEV).
Materiais e Métodos: Para a realização desta investigação laboratorial foram selecionados vinte e quatro molares permanentes humanos hígidos, extraídos há menos de seis meses do Banco de Dentes da Clínica Universitária Egas Moniz, e distribuídos aleatoriamente em três grupos consoante o protocolo de IDS aplicado: OptiBond™ FL (grupo de controlo) - etch-and-rinse de três passos (n=8), Clearfil™ SE Protect (grupo experimental) - self-etch de dois passos (n=8) e Clearfil™ SE Protect mais resina composta flowable (grupo experimental) (n=8). Cada grupo foi posteriormente dividido em dois subgrupos, de forma a serem estudados em dois tempos diferentes – ao imediato (24h) e após envelhecimento (3 meses). Em todos eles foi realizado o mesmo protocolo inicial, que consistiu no seccionamento dos dentes de forma a expor a dentina e produção da smear layer de forma artificial, para aplicação do respetivo protocolo de IDS. Foram aderidas restaurações provisórias em resina bis-acrílica e, posteriormente restaurações indiretas definitivas em resina composta. Por fim, as amostras foram seccionadas em espécimes com dimensões iguais, armazenadas em estufa a 37ºC durante o tempo experimental correspondente e analisadas através de MEV, de forma a caracterizar a
interface adesiva.
Resultados: Com a análise qualitativa das imagens obtidas por microscopia eletrónica de varrimento verificou-se a presença de uma interface adesiva bem definida, com a criação de uma camada híbrida espessa e uniforme para o IDS com OptiBond™ FL, tanto às 24h como aos 3 meses. No caso do IDS com recurso ao Clearfil™ SE Protect, verificou-se a criação de uma camada híbrida com dimensões muito reduzidas, que se manteve aos 3 meses.
Conclusão: Foram observadas camadas híbridas competentes para todos os grupos estudados, e que mantiveram a sua integridade após 3 meses de exposição direta a saliva artificial.
Purpose: To characterize and morphologically compare the adhesive interface obtained with different Immediate Dentin Sealing (IDS) protocols through Scanning Electron Microscopy (SEM). Materials and Methods: Twenty-four healthy human permanent molars, were selected to carry out this laboratory investigation, extracted less than six months ago from the Dental Bank of the Clínica Universitária Egas Moniz, and randomly divided into three groups according to the IDS protocol applied: OptiBond™ FL (control group) - threestep etch-and-rinse (n=8), Clearfil™ SE Protect (experimental group) - two-step self-etch (n=8) and Clearfil™ SE Protect plus flowable composite resin (experimental group) (n=8). Each group was later divided into two subgroups, in order to be studied at two different times – immediately (24h) and after aging (3 months). In all of them, the same initial protocol was performed, which consisted of sectioning the teeth in order to expose the dentin and artificially producing the smear layer, for the application of the respective IDS protocol. Provisional restorations in bis-acrylic resin were bonded and, later, definitive indirect restorations in composite resin. Finally, the samples were sectioned into specimens with equal dimensions, stored in oven at 37ºC during the corresponding experimental time and analyzed by SEM, in order to characterize the adhesive interface. Results: With the qualitative analysis of the images obtained by scanning electron microscopy, was verified the presence of a well-defined adhesive interface, with the creation of a thick and uniform hybrid layer for the IDS with OptiBond™ FL, both at 24h and at 3 months. In the case of IDS using Clearfil™ SE Protect, a hybrid layer with very small dimensions was created, which was maintained at 3 months. Conclusion: Competent hybrid layers were observed for all studied groups, which maintained their integrity after 3 months of direct exposure to artificial saliva.
Purpose: To characterize and morphologically compare the adhesive interface obtained with different Immediate Dentin Sealing (IDS) protocols through Scanning Electron Microscopy (SEM). Materials and Methods: Twenty-four healthy human permanent molars, were selected to carry out this laboratory investigation, extracted less than six months ago from the Dental Bank of the Clínica Universitária Egas Moniz, and randomly divided into three groups according to the IDS protocol applied: OptiBond™ FL (control group) - threestep etch-and-rinse (n=8), Clearfil™ SE Protect (experimental group) - two-step self-etch (n=8) and Clearfil™ SE Protect plus flowable composite resin (experimental group) (n=8). Each group was later divided into two subgroups, in order to be studied at two different times – immediately (24h) and after aging (3 months). In all of them, the same initial protocol was performed, which consisted of sectioning the teeth in order to expose the dentin and artificially producing the smear layer, for the application of the respective IDS protocol. Provisional restorations in bis-acrylic resin were bonded and, later, definitive indirect restorations in composite resin. Finally, the samples were sectioned into specimens with equal dimensions, stored in oven at 37ºC during the corresponding experimental time and analyzed by SEM, in order to characterize the adhesive interface. Results: With the qualitative analysis of the images obtained by scanning electron microscopy, was verified the presence of a well-defined adhesive interface, with the creation of a thick and uniform hybrid layer for the IDS with OptiBond™ FL, both at 24h and at 3 months. In the case of IDS using Clearfil™ SE Protect, a hybrid layer with very small dimensions was created, which was maintained at 3 months. Conclusion: Competent hybrid layers were observed for all studied groups, which maintained their integrity after 3 months of direct exposure to artificial saliva.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Immediate dentin sealing Camada híbrida Interface adesiva Restaurações indiretas