Publication
Microextraction by packed sorbent clean-up for the determination of selected synthetic cathinones in hair samples
| dc.contributor.author | Simão, A. | |
| dc.contributor.author | Margalho, Cláudia | |
| dc.contributor.author | Dinis, P. | |
| dc.contributor.author | Andraus, M. | |
| dc.contributor.author | Barroso, Mário | |
| dc.contributor.author | Gallardo, E. | |
| dc.date.accessioned | 2025-01-21T10:12:48Z | |
| dc.date.available | 2025-01-21T10:12:48Z | |
| dc.date.issued | 2024-09 | |
| dc.description | Poster apresentado no 61st Annual Meeting of the International Association of Forensic Toxicologists (TIAFT) St. Gallen, Suìça 2-6 Set 2024 | pt_PT |
| dc.description.abstract | Background and aims: The use of synthetic cathinones (SC), also known as "bath salts," has been increasing in recent years, leading to a growing concern about their effects on public health. Different clean-up approaches have already been studied by different authors. However, microextraction by packed sorbent (MEPS), a miniaturized form of solid-phase extraction, has not been explored extensively for the determination of SC in hair samples. Therefore, we propose the optimization of the MEPS procedure as sample clean-up method followed by gas chromatography mass spectrometry for the determination of these analytes in hair samples. Methods: Hair samples were washed consecutively with dichloromethane, deionized water and methanol to remove dirt and externally deposited material. After drying, hair was scissor-cut into small fragments. Fifty milligrams of hair were incubated overnight in the presence of 1 M NaOH at 45 °C, after which the extracts were neutralized and subjected to MEPS using a C8-SCX cartridge. The compounds were analyzed underivatized by GC-MS in the SIM mode. MEPS conditions were conditioning (250 μL of methanol followed by 250 μL of water), sample loading (24 x 700 μL of hair extract) and elution (7 x 100 μL of 2% NH4OH in acetonitrile). Results and discussion: The procedure resulted in acceptable recoveries (15-59% for all analytes), and allowed reaching lower limits of quantification (LLOQs) of 100 pg/mg for 4-MDMB, 4-MNEB and 4- MDMP and of 200 pg/mg for the remaining analytes (4-CEC, 4-MNEP, 4-MDEB, 4-MDMH, ethylone, alpha-PVP, penthylone, 4-Cl-PVP, MPHP and MDPV). Conclusions: In this work the optimization of the MEPS technique coupled to GC-MS for the determination of selected synthetic cathinones in hair samples is presented. The method was considered suitable for the analysis of these compounds in hair, providing acceptable recoveries and obtaining low limits of quantification. | pt_PT |
| dc.description.sponsorship | This work was partially supported by CICS-UBI, which is financed by National Funds from Fundação para a Ciência e a Tecnologia (FCT) and by Fundo Europeu de Desenvolvimento Regional (FEDER) under the scope of PORTUGAL 2020 and Programa Operacional do Centro (CENTRO 2020), with the project reference UIDB/00709/2020 and UIDP/ 00709/2020. Ana Y. Simão acknowledges the PhD fellowship from FCT (Reference: 2020.09070.BD | pt_PT |
| dc.description.version | N/A | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.26/53926 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.relation | Health Sciences Research Centre | |
| dc.relation | Health Sciences Research Centre | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | MEPS | pt_PT |
| dc.subject | Hair | pt_PT |
| dc.subject | Synthetic Cathinones | pt_PT |
| dc.subject | GC-MS | pt_PT |
| dc.title | Microextraction by packed sorbent clean-up for the determination of selected synthetic cathinones in hair samples | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Health Sciences Research Centre | |
| oaire.awardTitle | Health Sciences Research Centre | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT | |
| oaire.citation.conferencePlace | St. Gallen, SUÍÇA | pt_PT |
| oaire.citation.title | 61st Annual Meeting of the International Association of Forensic Toxicologists (TIAFT) | pt_PT |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| person.familyName | Margalho | |
| person.familyName | Barroso | |
| person.givenName | Cláudia | |
| person.givenName | Mário | |
| person.identifier.ciencia-id | A211-521D-90CA | |
| person.identifier.ciencia-id | 5110-F476-ECCC | |
| person.identifier.orcid | 0000-0003-1661-0367 | |
| person.identifier.orcid | 0000-0001-8848-2734 | |
| person.identifier.rid | KII-2916-2024 | |
| person.identifier.scopus-author-id | 8655072100 | |
| person.identifier.scopus-author-id | 35329243100 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
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