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Avaliação da influência do ácido ftalimidoperoxicaproico (PAP) na integridade do esmalte e da dentina
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Objetivo: O objetivo deste estudo foi avaliar alterações na microdureza e na superfície do esmalte e da dentina quando sujeitos à ação de um gel à base de PAP.
Materiais e métodos: Foram utilizados 9 dentes hígidos extraídos há menos de 6 meses e recolhidos do banco de dentes da Clínica Universitária Egas Moniz. O ácido ftalimidoperoxicaproico (PAP) (Brilliant Lumina, Coltene) foi aplicado na superfície vestibular de cada dente segundo as instruções do fabricante. Em cada dente foram efetuados quatro cortes, no sentido mésio-distal, com 2 mm de espessura, para obter uma fatia de esmalte vestibular, de dentina vestibular, de esmalte lingual e de dentina lingual, e assim formar quatro grupos de estudo (n=9): a) esmalte teste (onde foi aplicado o PAP), b) dentina teste (onde foi aplicado o PAP), c) dentina controlo, d) esmalte controlo, ou seja 18 amostras de controlo e 18 amostras de teste. Estes cortes foram realizados com um micrótomo automático. As amostras foram submetidas ao teste de microdureza de Vickers e duas de cada grupo foram observadas ao MEV .Os resultados de microdureza foram tratados estatisticamente com um teste t de Student para compar os valores médios de microdureza entre os grupos experimentais e os grupos de controlo. Considerou-se um nível de significância de 5 %.
Resultados: O teste de microdureza de Vickers demostrou no Grupo a) uma média de dureza significativamente inferior em comparação com o Grupo d). Não se verificou nenhuma diferença entre os grupos b) e c). Com o microscópio eletrónico de varrimento verificou-se um aumento significativo na rugosidade superficial entre os Grupos a) e d). No entanto, não foram identificadas diferenças entre os grupos b) e c).
Conclusões: A aplicação do PAP resultou numa redução significativa da microdureza do esmalte, assim como num aumento da sua rugosidade superficial. Em contraste, não foram observadas alterações na dentina. Deste modo, o PAP não pode ser considerado um agente inofensivo para o esmalte.
Objective: The aim of this study was to evaluate changes in the microhardness and surface of enamel and dentin when subjected to the action of a PAP-based gel. Materials and methods: 9 healthy teeth extracted less than 6 months previously and collected from the Egas Moniz University tooth bank were used. The PAP (Brilliant Lumina, Coltene) was applied to the buccal surface of each tooth following the manufacturer's instructions. For each tooth, four 2 mm thick sections were cut in the mesio-distal direction to obtain a slice of buccal enamel, buccal dentin, lingual enamel and lingual dentin from each tooth, thus forming four study groups (n=9): a) test enamel (where PAP was applied), b) test dentin enamel (where PAP was applied), c) control dentin, d) control enamel. The cuts were made using an automatic microtome. The samples were subjected to the Vickers microhardness test, and two from each group were observed under SEM (Scanning Electron Microscopy). The microhardness results were statistically analyzed using a Student's t-test to compare the mean microhardness values between the experimental groups and the control groups. It was considered a significance level of 5%. Results: The Vickers microhardness test showed that Group a) had a significantly lower average hardness compared to Group d). No differences were observed between Groups b) and c). Scanning electron microscopy revealed a significant increase in surface roughness between Groups a) and d). However, no differences were identified between Groups b) and c). Conclusions: The application of PAP led to a significant reduction in enamel microhardness, as well as an increase in its surface roughness. In contrast, no changes were observed in the dentin. Therefore, PAP cannot be considered a harmless agent for dental enamel.
Objective: The aim of this study was to evaluate changes in the microhardness and surface of enamel and dentin when subjected to the action of a PAP-based gel. Materials and methods: 9 healthy teeth extracted less than 6 months previously and collected from the Egas Moniz University tooth bank were used. The PAP (Brilliant Lumina, Coltene) was applied to the buccal surface of each tooth following the manufacturer's instructions. For each tooth, four 2 mm thick sections were cut in the mesio-distal direction to obtain a slice of buccal enamel, buccal dentin, lingual enamel and lingual dentin from each tooth, thus forming four study groups (n=9): a) test enamel (where PAP was applied), b) test dentin enamel (where PAP was applied), c) control dentin, d) control enamel. The cuts were made using an automatic microtome. The samples were subjected to the Vickers microhardness test, and two from each group were observed under SEM (Scanning Electron Microscopy). The microhardness results were statistically analyzed using a Student's t-test to compare the mean microhardness values between the experimental groups and the control groups. It was considered a significance level of 5%. Results: The Vickers microhardness test showed that Group a) had a significantly lower average hardness compared to Group d). No differences were observed between Groups b) and c). Scanning electron microscopy revealed a significant increase in surface roughness between Groups a) and d). However, no differences were identified between Groups b) and c). Conclusions: The application of PAP led to a significant reduction in enamel microhardness, as well as an increase in its surface roughness. In contrast, no changes were observed in the dentin. Therefore, PAP cannot be considered a harmless agent for dental enamel.
Descrição
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Palavras-chave
Branqueamento dentário Erosão dentária Microdureza Ácido ftalimidoperoxicaproico